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Inhibitory effects of active substances isolated from Cassia garrettiana heartwood on tumor growth and lung metastasis in Lewis lung carcinoma-bearing mice (Part 2).
Anticancer Res 2000 Sep-Oct; 20(5A):2923-30AR

Abstract

Previously, we reported that a methanol extract (500 mg/kg x 2/day) of the heartwood of Cassia garrettiana inhibited the tumor growth and metastasis to the lung in Lewis lung carcinoma (LLC)-bearing mice. Furthermore, we isolated the two active substances from the methanol extract of C. garrettiana and identified compound 1 as cassigarol A. In the present study, compound 2 was identified as 3, 3', 4, 5'-tetrahydroxy stilbene (piceatannol) based on the 1H-NMR spectral data and products formed by oxidation with potassium permanganate. We examined the active substance (compound 2, piceatannol) and its acetylated derivative on the tumor growth and lung metastasis in LLC-bearing and carcinectomized mice. Piceatannol (50 mg and 100 mg/kg x 2/day) did not affect the tumor growth, while piceatannol acetate (50 mg and 100 mg/kg x 2/day) significantly inhibited the tumor growth. Piceatannol and its derivative piceatannol acetate inhibited the metastasis to the lung dose-dependently in carcinectomized mice. Moreover, piceatannol and piceatannol acetate prolonged the survival time and increased the survival rate in carcinectomized mice. In addition, piceatannol inhibited the formation of capillary-like networks of human umbilical vein endothelial cells (HUVECs) at the concentrations of 10 to 100 microM, but its acetylated derivative did not. Therefore, it is suggested that the antimetastatic activities of piceatannol might be due to the inhibition of tube formation (angiogenesis) of HUVECs.

Authors+Show Affiliations

Second Department of Medical Biochemistry, School of Medicine, Ehime University, Japan. yokim@m.ehime-u.ac.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11062702

Citation

Kimura, Y, et al. "Inhibitory Effects of Active Substances Isolated From Cassia Garrettiana Heartwood On Tumor Growth and Lung Metastasis in Lewis Lung Carcinoma-bearing Mice (Part 2)." Anticancer Research, vol. 20, no. 5A, 2000, pp. 2923-30.
Kimura Y, Baba K, Okuda H. Inhibitory effects of active substances isolated from Cassia garrettiana heartwood on tumor growth and lung metastasis in Lewis lung carcinoma-bearing mice (Part 2). Anticancer Res. 2000;20(5A):2923-30.
Kimura, Y., Baba, K., & Okuda, H. (2000). Inhibitory effects of active substances isolated from Cassia garrettiana heartwood on tumor growth and lung metastasis in Lewis lung carcinoma-bearing mice (Part 2). Anticancer Research, 20(5A), pp. 2923-30.
Kimura Y, Baba K, Okuda H. Inhibitory Effects of Active Substances Isolated From Cassia Garrettiana Heartwood On Tumor Growth and Lung Metastasis in Lewis Lung Carcinoma-bearing Mice (Part 2). Anticancer Res. 2000;20(5A):2923-30. PubMed PMID: 11062702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of active substances isolated from Cassia garrettiana heartwood on tumor growth and lung metastasis in Lewis lung carcinoma-bearing mice (Part 2). AU - Kimura,Y, AU - Baba,K, AU - Okuda,H, PY - 2000/11/4/pubmed PY - 2001/2/28/medline PY - 2000/11/4/entrez SP - 2923 EP - 30 JF - Anticancer research JO - Anticancer Res. VL - 20 IS - 5A N2 - Previously, we reported that a methanol extract (500 mg/kg x 2/day) of the heartwood of Cassia garrettiana inhibited the tumor growth and metastasis to the lung in Lewis lung carcinoma (LLC)-bearing mice. Furthermore, we isolated the two active substances from the methanol extract of C. garrettiana and identified compound 1 as cassigarol A. In the present study, compound 2 was identified as 3, 3', 4, 5'-tetrahydroxy stilbene (piceatannol) based on the 1H-NMR spectral data and products formed by oxidation with potassium permanganate. We examined the active substance (compound 2, piceatannol) and its acetylated derivative on the tumor growth and lung metastasis in LLC-bearing and carcinectomized mice. Piceatannol (50 mg and 100 mg/kg x 2/day) did not affect the tumor growth, while piceatannol acetate (50 mg and 100 mg/kg x 2/day) significantly inhibited the tumor growth. Piceatannol and its derivative piceatannol acetate inhibited the metastasis to the lung dose-dependently in carcinectomized mice. Moreover, piceatannol and piceatannol acetate prolonged the survival time and increased the survival rate in carcinectomized mice. In addition, piceatannol inhibited the formation of capillary-like networks of human umbilical vein endothelial cells (HUVECs) at the concentrations of 10 to 100 microM, but its acetylated derivative did not. Therefore, it is suggested that the antimetastatic activities of piceatannol might be due to the inhibition of tube formation (angiogenesis) of HUVECs. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/11062702/Inhibitory_effects_of_active_substances_isolated_from_Cassia_garrettiana_heartwood_on_tumor_growth_and_lung_metastasis_in_Lewis_lung_carcinoma_bearing_mice__Part_2__ L2 - https://medlineplus.gov/lungcancer.html DB - PRIME DP - Unbound Medicine ER -