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The isolation and characterization of a strain of subacute sclerosing panencephalitis measles-related virus.
Ann Microbiol (Paris). 1975 May-Jun; 126A(4):509-21.AM

Abstract

The isolation of a measles-like virus in CV-1 cell cultures from a biopsy specimen obtained from a case of subacute sclerosing panencephalitis (SSPE) is reported. Evidence of the presence of cell-free virus was obtained from the fifth cell culture passage of the isolated virus. The virus was identified as measles-related by standard immune sera. IF study did not show relevant differences between the isolated virus and the Edmonston strain of measles virus, even though the proportion of cells showing nuclear antigen was higher following the infection with the isolated virus than with the Edmonston strain. Electron microscopy study failed to demonstrate viral structures in the cells from the brain biopsy, while numerous large aggregates of nucleocapsids were found in the cytoplasm of infected cells observed on the third and fourth passage in tissue culture. The neuropathogenicity assay carried out on newborn hamsters, rats and mice using a suspension of disrupted infected cells did not show any clinical pathological effect due to the SSPE strain. These results are partially in contrast with previous findings which indicate relevant differences in the morphology and pathogenicity of SSPE derived strains as compared to reference measles virus.

Authors

de Felici A
No affiliation info available
de Lellis M
No affiliation info available
Rocchi G
No affiliation info available
Archetti I
No affiliation info available

MeSH

Antibodies, ViralBrainCells, CulturedChildCytopathogenic Effect, ViralFluorescent Antibody TechniqueHemadsorption Inhibition TestsHumansMaleMeasles virusNeutralization TestsOrthomyxoviridaeSubacute Sclerosing Panencephalitis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1106294

Citation

de Felici, A, et al. "The Isolation and Characterization of a Strain of Subacute Sclerosing Panencephalitis Measles-related Virus." Annales De Microbiologie, vol. 126A, no. 4, 1975, pp. 509-21.
de Felici A, de Lellis M, Rocchi G, et al. The isolation and characterization of a strain of subacute sclerosing panencephalitis measles-related virus. Ann Microbiol (Paris). 1975;126A(4):509-21.
de Felici, A., de Lellis, M., Rocchi, G., & Archetti, I. (1975). The isolation and characterization of a strain of subacute sclerosing panencephalitis measles-related virus. Annales De Microbiologie, 126A(4), 509-21.
de Felici A, et al. The Isolation and Characterization of a Strain of Subacute Sclerosing Panencephalitis Measles-related Virus. Ann Microbiol (Paris). 1975 May-Jun;126A(4):509-21. PubMed PMID: 1106294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The isolation and characterization of a strain of subacute sclerosing panencephalitis measles-related virus. AU - de Felici,A, AU - de Lellis,M, AU - Rocchi,G, AU - Archetti,I, PY - 1975/5/1/pubmed PY - 1975/5/1/medline PY - 1975/5/1/entrez SP - 509 EP - 21 JF - Annales de microbiologie JO - Ann Microbiol (Paris) VL - 126A IS - 4 N2 - The isolation of a measles-like virus in CV-1 cell cultures from a biopsy specimen obtained from a case of subacute sclerosing panencephalitis (SSPE) is reported. Evidence of the presence of cell-free virus was obtained from the fifth cell culture passage of the isolated virus. The virus was identified as measles-related by standard immune sera. IF study did not show relevant differences between the isolated virus and the Edmonston strain of measles virus, even though the proportion of cells showing nuclear antigen was higher following the infection with the isolated virus than with the Edmonston strain. Electron microscopy study failed to demonstrate viral structures in the cells from the brain biopsy, while numerous large aggregates of nucleocapsids were found in the cytoplasm of infected cells observed on the third and fourth passage in tissue culture. The neuropathogenicity assay carried out on newborn hamsters, rats and mice using a suspension of disrupted infected cells did not show any clinical pathological effect due to the SSPE strain. These results are partially in contrast with previous findings which indicate relevant differences in the morphology and pathogenicity of SSPE derived strains as compared to reference measles virus. SN - 0300-5410 UR - https://www.unboundmedicine.com/medline/citation/1106294/The_isolation_and_characterization_of_a_strain_of_subacute_sclerosing_panencephalitis_measles_related_virus_ DB - PRIME DP - Unbound Medicine ER -