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The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects.
Microbiology 2000; 146 (Pt 11):2825-2832M

Abstract

Bacillus thuringiensis has been widely used for 40 years as a safe biopesticide for controlling agricultural pests and mosquitoes because it produces insecticidal crystal proteins. However, spores have also been shown to contribute to overall entomopathogenicity. Here, the opportunistic properties of acrystalliferous B. thuringiensis Cry(-) and Bacillus cereus strains were investigated in an insect species, Galleria mellonella, and in a mammal, BALB/c mice. In both animal models, the pathogenicity of the two bacterial species was similar. Mutant strains were constructed in which the plcR gene, encoding a pleiotropic regulator of extracellular factors, was disrupted. In larvae, co-ingestion of 10(6) spores of the parental strain with a sublethal concentration of Cry1C toxin caused 70% mortality whereas only 7% mortality was recorded if spores of the DeltaplcR mutant strain were used. In mice, nasal instillation of 10(8) spores of the parental strain caused 100% mortality whereas instillation with the same number of DeltaplcR strain spores caused much lower or no mortality. Similar effects were obtained if vegetative cells were used instead of spores. The cause of death is unknown and is unlikely to be due to actual growth of the bacteria in mice. The lesions caused by B. thuringiensis supernatant in infected mice suggested that haemolytic toxins were involved. The cytolytic properties of strains of B. thuringiensis and B. cereus, using sheep, horse and human erythrocytes and G. mellonella haemocytes, were therefore investigated. The level of cytolytic activity is highly reduced in DeltaplcR strains. Together, the results indicate that the pathogenicity of B. thuringiensis strain 407 and B. cereus strain ATCC 14579 is controlled by PlcR.

Authors+Show Affiliations

Unité de Lutte Biologique, INRA, La Minière, 78285 Guyancourt cedex, France2. Unité de Biochimie Microbienne, CNRS (URA2172), Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex, France1.Centre d'Etudes du Bouchet, Laboratoire de Microbiologie, 91710 Vert-Le-Petit, France3.Laboratoire de Pathologie Comparée, INRA-CNRS (URA 2209), Université Montpellier II, 34095 Montpellier, France4.Unité de Lutte Biologique, INRA, La Minière, 78285 Guyancourt cedex, France2.Unité de Lutte Biologique, INRA, La Minière, 78285 Guyancourt cedex, France2.Unité de Biochimie Microbienne, CNRS (URA2172), Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex, France1.Laboratoire de Biologie, HIA Bégin, 94160 Saint Mandé, France5.Unité de Lutte Biologique, INRA, La Minière, 78285 Guyancourt cedex, France2. Unité de Biochimie Microbienne, CNRS (URA2172), Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex, France1.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11065361

Citation

Salamitou, Sylvie, et al. "The plcR Regulon Is Involved in the Opportunistic Properties of Bacillus Thuringiensis and Bacillus Cereus in Mice and Insects." Microbiology (Reading, England), vol. 146 (Pt 11), 2000, pp. 2825-2832.
Salamitou S, Ramisse F, Brehélin M, et al. The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects. Microbiology (Reading, Engl). 2000;146 (Pt 11):2825-2832.
Salamitou, S., Ramisse, F., Brehélin, M., Bourguet, D., Gilois, N., Gominet, M., ... Lereclus, D. (2000). The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects. Microbiology (Reading, England), 146 (Pt 11), pp. 2825-2832. doi:10.1099/00221287-146-11-2825.
Salamitou S, et al. The plcR Regulon Is Involved in the Opportunistic Properties of Bacillus Thuringiensis and Bacillus Cereus in Mice and Insects. Microbiology (Reading, Engl). 2000;146 (Pt 11):2825-2832. PubMed PMID: 11065361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects. AU - Salamitou,Sylvie, AU - Ramisse,Françoise, AU - Brehélin,Michel, AU - Bourguet,Denis, AU - Gilois,Nathalie, AU - Gominet,Myriam, AU - Hernandez,Eric, AU - Lereclus,Didier, PY - 2000/11/7/pubmed PY - 2001/2/28/medline PY - 2000/11/7/entrez SP - 2825 EP - 2832 JF - Microbiology (Reading, England) JO - Microbiology (Reading, Engl.) VL - 146 (Pt 11) N2 - Bacillus thuringiensis has been widely used for 40 years as a safe biopesticide for controlling agricultural pests and mosquitoes because it produces insecticidal crystal proteins. However, spores have also been shown to contribute to overall entomopathogenicity. Here, the opportunistic properties of acrystalliferous B. thuringiensis Cry(-) and Bacillus cereus strains were investigated in an insect species, Galleria mellonella, and in a mammal, BALB/c mice. In both animal models, the pathogenicity of the two bacterial species was similar. Mutant strains were constructed in which the plcR gene, encoding a pleiotropic regulator of extracellular factors, was disrupted. In larvae, co-ingestion of 10(6) spores of the parental strain with a sublethal concentration of Cry1C toxin caused 70% mortality whereas only 7% mortality was recorded if spores of the DeltaplcR mutant strain were used. In mice, nasal instillation of 10(8) spores of the parental strain caused 100% mortality whereas instillation with the same number of DeltaplcR strain spores caused much lower or no mortality. Similar effects were obtained if vegetative cells were used instead of spores. The cause of death is unknown and is unlikely to be due to actual growth of the bacteria in mice. The lesions caused by B. thuringiensis supernatant in infected mice suggested that haemolytic toxins were involved. The cytolytic properties of strains of B. thuringiensis and B. cereus, using sheep, horse and human erythrocytes and G. mellonella haemocytes, were therefore investigated. The level of cytolytic activity is highly reduced in DeltaplcR strains. Together, the results indicate that the pathogenicity of B. thuringiensis strain 407 and B. cereus strain ATCC 14579 is controlled by PlcR. SN - 1350-0872 UR - https://www.unboundmedicine.com/medline/citation/11065361/The_plcR_regulon_is_involved_in_the_opportunistic_properties_of_Bacillus_thuringiensis_and_Bacillus_cereus_in_mice_and_insects_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/00221287-146-11-2825 DB - PRIME DP - Unbound Medicine ER -