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Indole-3-glycerol phosphate, a branchpoint of indole-3-acetic acid biosynthesis from the tryptophan biosynthetic pathway in Arabidopsis thaliana.
Plant J. 2000 Nov; 24(3):327-33.PJ

Abstract

The phytohormone indole-3-acetic acid (IAA) plays a vital role in plant growth and development as a regulator of numerous biological processes. Its biosynthetic pathways have been studied for decades. Recent genetic and in vitro labeling evidence indicates that IAA in Arabidopsis thaliana and other plants is primarily synthesized from a precursor that is an intermediate in the tryptophan (Trp) biosynthetic pathway. To determine which intermediate(s) acts as the possible branchpoint for the Trp-independent IAA biosynthesis in plants, we took an in vivo approach by generating antisense indole-3-glycerol phosphate synthase (IGS) RNA transgenic plants and using available Arabidopsis Trp biosynthetic pathway mutants trp2-1 and trp3-1. Antisense transgenic plants display some auxin deficient-like phenotypes including small rosettes and reduced fertility. Protein gel blot analysis indicated that IGS expression was greatly reduced in the antisense lines. Quantitative analyses of IAA and Trp content in antisense IGS transgenic plants and Trp biosynthetic mutants revealed striking differences. Compared with wild-type plants, the Trp content in all the transgenic and mutant plants decreased significantly. However, total IAA levels were significantly decreased in antisense IGS transgenic plants, but remarkably increased in trp3-1 and trp2-1 plants. These results suggest that indole-3-glycerol phosphate (IGP) in the Arabidopsis Trp biosynthetic pathway serves as a branchpoint compound in the Trp-independent IAA de novo biosynthetic pathway.

Authors+Show Affiliations

Ouyang J
Institute of Genetics, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101, China.
Shao X
No affiliation info available
Li J
No affiliation info available

MeSH

ArabidopsisGenes, PlantGlycerophosphatesIndoleacetic AcidsMutationPlants, Genetically ModifiedRNA, AntisenseTryptophan

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11069706

Citation

Ouyang, J, et al. "Indole-3-glycerol Phosphate, a Branchpoint of Indole-3-acetic Acid Biosynthesis From the Tryptophan Biosynthetic Pathway in Arabidopsis Thaliana." The Plant Journal : for Cell and Molecular Biology, vol. 24, no. 3, 2000, pp. 327-33.
Ouyang J, Shao X, Li J. Indole-3-glycerol phosphate, a branchpoint of indole-3-acetic acid biosynthesis from the tryptophan biosynthetic pathway in Arabidopsis thaliana. Plant J. 2000;24(3):327-33.
Ouyang, J., Shao, X., & Li, J. (2000). Indole-3-glycerol phosphate, a branchpoint of indole-3-acetic acid biosynthesis from the tryptophan biosynthetic pathway in Arabidopsis thaliana. The Plant Journal : for Cell and Molecular Biology, 24(3), 327-33.
Ouyang J, Shao X, Li J. Indole-3-glycerol Phosphate, a Branchpoint of Indole-3-acetic Acid Biosynthesis From the Tryptophan Biosynthetic Pathway in Arabidopsis Thaliana. Plant J. 2000;24(3):327-33. PubMed PMID: 11069706.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Indole-3-glycerol phosphate, a branchpoint of indole-3-acetic acid biosynthesis from the tryptophan biosynthetic pathway in Arabidopsis thaliana. AU - Ouyang,J, AU - Shao,X, AU - Li,J, PY - 2000/11/9/pubmed PY - 2001/3/3/medline PY - 2000/11/9/entrez SP - 327 EP - 33 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 24 IS - 3 N2 - The phytohormone indole-3-acetic acid (IAA) plays a vital role in plant growth and development as a regulator of numerous biological processes. Its biosynthetic pathways have been studied for decades. Recent genetic and in vitro labeling evidence indicates that IAA in Arabidopsis thaliana and other plants is primarily synthesized from a precursor that is an intermediate in the tryptophan (Trp) biosynthetic pathway. To determine which intermediate(s) acts as the possible branchpoint for the Trp-independent IAA biosynthesis in plants, we took an in vivo approach by generating antisense indole-3-glycerol phosphate synthase (IGS) RNA transgenic plants and using available Arabidopsis Trp biosynthetic pathway mutants trp2-1 and trp3-1. Antisense transgenic plants display some auxin deficient-like phenotypes including small rosettes and reduced fertility. Protein gel blot analysis indicated that IGS expression was greatly reduced in the antisense lines. Quantitative analyses of IAA and Trp content in antisense IGS transgenic plants and Trp biosynthetic mutants revealed striking differences. Compared with wild-type plants, the Trp content in all the transgenic and mutant plants decreased significantly. However, total IAA levels were significantly decreased in antisense IGS transgenic plants, but remarkably increased in trp3-1 and trp2-1 plants. These results suggest that indole-3-glycerol phosphate (IGP) in the Arabidopsis Trp biosynthetic pathway serves as a branchpoint compound in the Trp-independent IAA de novo biosynthetic pathway. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/11069706/Indole_3_glycerol_phosphate_a_branchpoint_of_indole_3_acetic_acid_biosynthesis_from_the_tryptophan_biosynthetic_pathway_in_Arabidopsis_thaliana_ DB - PRIME DP - Unbound Medicine ER -