Tags

Type your tag names separated by a space and hit enter

Inducible nitric oxide synthase-knockout mice exhibit resistance to pleurisy and lung injury caused by carrageenan.
Am J Respir Crit Care Med. 2000 Nov; 162(5):1859-66.AJ

Abstract

In the present study, we investigated the role of inducible (or type 2) nitric oxide synthase (iNOS) in the development of acute inflammation by comparing the responses in wild-type mice (WT) and mice lacking (knockout [KO]). When compared with carrageenan-treated iNOS-WT mice, iNOS-KO mice that had received carrageenan exhibited a reduced degree of pleural exudation and polymorphonuclear cell migration. Lung myeloperoxidase (MPO) activity and lipid peroxidation were significantly reduced in iNOS-KO mice in comparison with iNOSWT mice. Immunohistochemical analysis for nitrotyrosine revealed positive staining in lungs from carrageenan-treated iNOS-WT mice. Lung tissue sections from carrageenan-treated iNOS-WT mice showed positive staining for poly adenosine diphosphate (ADP)-ribose synthetase that was mainly localized in alveolar macrophages and in airway epithelial cells. The intensity and degree of staining for nitrotyrosine and poly-ADP-ribose synthetase were markedly reduced in tissue sections from carrageenan-treated iNOS-KO mice. The inflamed lungs of iNOS-KO mice also showed an improved histologic status. Furthermore, a significant reduction in the suppression of energy status, in DNA strand breakage, and in decreased cellular levels of nicotinamide adenine dinucleotide (NAD(+)) was observed ex vivo in macrophages harvested from the pleural cavity of iNOS-KO mice subjected to carrageenan-induced pleurisy. Taken together, our results clearly show that iNOS plays an important role in the acute inflammatory response.

Authors+Show Affiliations

Institute of Pharmacology and Department of Biomorphology School of Medicine, University of Messina, Messina, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11069827

Citation

Cuzzocrea, S, et al. "Inducible Nitric Oxide Synthase-knockout Mice Exhibit Resistance to Pleurisy and Lung Injury Caused By Carrageenan." American Journal of Respiratory and Critical Care Medicine, vol. 162, no. 5, 2000, pp. 1859-66.
Cuzzocrea S, Mazzon E, Calabro G, et al. Inducible nitric oxide synthase-knockout mice exhibit resistance to pleurisy and lung injury caused by carrageenan. Am J Respir Crit Care Med. 2000;162(5):1859-66.
Cuzzocrea, S., Mazzon, E., Calabro, G., Dugo, L., De Sarro, A., van De LOO, F. A., & Caputi, A. P. (2000). Inducible nitric oxide synthase-knockout mice exhibit resistance to pleurisy and lung injury caused by carrageenan. American Journal of Respiratory and Critical Care Medicine, 162(5), 1859-66.
Cuzzocrea S, et al. Inducible Nitric Oxide Synthase-knockout Mice Exhibit Resistance to Pleurisy and Lung Injury Caused By Carrageenan. Am J Respir Crit Care Med. 2000;162(5):1859-66. PubMed PMID: 11069827.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inducible nitric oxide synthase-knockout mice exhibit resistance to pleurisy and lung injury caused by carrageenan. AU - Cuzzocrea,S, AU - Mazzon,E, AU - Calabro,G, AU - Dugo,L, AU - De Sarro,A, AU - van De LOO,F A, AU - Caputi,A P, PY - 2000/11/9/pubmed PY - 2001/2/28/medline PY - 2000/11/9/entrez SP - 1859 EP - 66 JF - American journal of respiratory and critical care medicine JO - Am J Respir Crit Care Med VL - 162 IS - 5 N2 - In the present study, we investigated the role of inducible (or type 2) nitric oxide synthase (iNOS) in the development of acute inflammation by comparing the responses in wild-type mice (WT) and mice lacking (knockout [KO]). When compared with carrageenan-treated iNOS-WT mice, iNOS-KO mice that had received carrageenan exhibited a reduced degree of pleural exudation and polymorphonuclear cell migration. Lung myeloperoxidase (MPO) activity and lipid peroxidation were significantly reduced in iNOS-KO mice in comparison with iNOSWT mice. Immunohistochemical analysis for nitrotyrosine revealed positive staining in lungs from carrageenan-treated iNOS-WT mice. Lung tissue sections from carrageenan-treated iNOS-WT mice showed positive staining for poly adenosine diphosphate (ADP)-ribose synthetase that was mainly localized in alveolar macrophages and in airway epithelial cells. The intensity and degree of staining for nitrotyrosine and poly-ADP-ribose synthetase were markedly reduced in tissue sections from carrageenan-treated iNOS-KO mice. The inflamed lungs of iNOS-KO mice also showed an improved histologic status. Furthermore, a significant reduction in the suppression of energy status, in DNA strand breakage, and in decreased cellular levels of nicotinamide adenine dinucleotide (NAD(+)) was observed ex vivo in macrophages harvested from the pleural cavity of iNOS-KO mice subjected to carrageenan-induced pleurisy. Taken together, our results clearly show that iNOS plays an important role in the acute inflammatory response. SN - 1073-449X UR - https://www.unboundmedicine.com/medline/citation/11069827/Inducible_nitric_oxide_synthase_knockout_mice_exhibit_resistance_to_pleurisy_and_lung_injury_caused_by_carrageenan_ L2 - https://www.atsjournals.org/doi/10.1164/ajrccm.162.5.9912125?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -