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Characteristic elevation of matrix metalloproteinase activity in idiopathic interstitial pneumonias.
Am J Respir Crit Care Med. 2000 Nov; 162(5):1949-56.AJ

Abstract

Destruction of subepithelial basement membrane is a key event in the pathogenesis of idiopathic pulmonary fibrosis (IPF). To evaluate the role of matrix metalloproteinases (MMPs) in parenchymal remodeling in idiopathic interstitial pneumonia (IIP), we studied MMP-2 and -9 activity, in bronchoalveolar lavage fluid (BALF) by zymography and the expression of MMP-2 and -9 and TIMP-2 in lung tissue by immunohistochemistry. BALF and lung tissues were collected from 26 patients with usual interstitial pneumonia (IPF-UIP), 11 with nonspecific interstitial pneumonia (NSIP), and 6 with bronchiolitis obliterans organizing pneumonia (BOOP). IPF-UIP cases showed predominant expression of MMP-9, whereas NSIP and BOOP cases showed predominant MMP-2 expression in BALF and in tissues. In BALF samples from rapidly progressive IPF-UIP cases, neutrophil-derived MMP-9 activity, as well as MMP-9 active form were characteristically detected. Furthermore, the MMP-9 activity correlated significantly with an increase of neutrophils in BALF, whereas the MMP-2 activity associated with NSIP and BOOP correlated with an increase of lymphocytes. These results indicate that MMP-9 in IPF-UIP and MMP-2 in NSIP and BOOP may contribute to pulmonary structural remodeling through type IV collagenolytic activity. The characteristic contributions of matrix-degrading proteins may relate to the distinct prognostic features of these diseases.

Authors+Show Affiliations

First Department of Internal Medicine and Department of Microbiology, Kumamoto University School of Medicine, Kumamoto, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11069839

Citation

Suga, M, et al. "Characteristic Elevation of Matrix Metalloproteinase Activity in Idiopathic Interstitial Pneumonias." American Journal of Respiratory and Critical Care Medicine, vol. 162, no. 5, 2000, pp. 1949-56.
Suga M, Iyonaga K, Okamoto T, et al. Characteristic elevation of matrix metalloproteinase activity in idiopathic interstitial pneumonias. Am J Respir Crit Care Med. 2000;162(5):1949-56.
Suga, M., Iyonaga, K., Okamoto, T., Gushima, Y., Miyakawa, H., Akaike, T., & Ando, M. (2000). Characteristic elevation of matrix metalloproteinase activity in idiopathic interstitial pneumonias. American Journal of Respiratory and Critical Care Medicine, 162(5), 1949-56.
Suga M, et al. Characteristic Elevation of Matrix Metalloproteinase Activity in Idiopathic Interstitial Pneumonias. Am J Respir Crit Care Med. 2000;162(5):1949-56. PubMed PMID: 11069839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristic elevation of matrix metalloproteinase activity in idiopathic interstitial pneumonias. AU - Suga,M, AU - Iyonaga,K, AU - Okamoto,T, AU - Gushima,Y, AU - Miyakawa,H, AU - Akaike,T, AU - Ando,M, PY - 2000/11/9/pubmed PY - 2001/2/28/medline PY - 2000/11/9/entrez SP - 1949 EP - 56 JF - American journal of respiratory and critical care medicine JO - Am J Respir Crit Care Med VL - 162 IS - 5 N2 - Destruction of subepithelial basement membrane is a key event in the pathogenesis of idiopathic pulmonary fibrosis (IPF). To evaluate the role of matrix metalloproteinases (MMPs) in parenchymal remodeling in idiopathic interstitial pneumonia (IIP), we studied MMP-2 and -9 activity, in bronchoalveolar lavage fluid (BALF) by zymography and the expression of MMP-2 and -9 and TIMP-2 in lung tissue by immunohistochemistry. BALF and lung tissues were collected from 26 patients with usual interstitial pneumonia (IPF-UIP), 11 with nonspecific interstitial pneumonia (NSIP), and 6 with bronchiolitis obliterans organizing pneumonia (BOOP). IPF-UIP cases showed predominant expression of MMP-9, whereas NSIP and BOOP cases showed predominant MMP-2 expression in BALF and in tissues. In BALF samples from rapidly progressive IPF-UIP cases, neutrophil-derived MMP-9 activity, as well as MMP-9 active form were characteristically detected. Furthermore, the MMP-9 activity correlated significantly with an increase of neutrophils in BALF, whereas the MMP-2 activity associated with NSIP and BOOP correlated with an increase of lymphocytes. These results indicate that MMP-9 in IPF-UIP and MMP-2 in NSIP and BOOP may contribute to pulmonary structural remodeling through type IV collagenolytic activity. The characteristic contributions of matrix-degrading proteins may relate to the distinct prognostic features of these diseases. SN - 1073-449X UR - https://www.unboundmedicine.com/medline/citation/11069839/Characteristic_elevation_of_matrix_metalloproteinase_activity_in_idiopathic_interstitial_pneumonias_ L2 - https://www.atsjournals.org/doi/10.1164/ajrccm.162.5.9906096?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -