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Increased density of lymphocytes bearing gamma/delta T-cell receptors in recurrent aphthous ulceration (RAU).
Int J Oral Maxillofac Surg. 2000 Oct; 29(5):375-80.IJ

Abstract

Lymphocytes bearing the T-cell receptor (TCR) gamma/delta are increased in the peripheral blood of patients with recurrent aphthous ulcers (RAU) and Behcet's disease. In this study, we examined whether the density of TCR-gamma/delta bearing lymphocytes was also increased locally in RAU lesions. Ten RAU lesions from ten patients were compared with ulcer-free mucosa from sites contralateral to the lesions, and with 10 samples of clinically healthy oral mucosa taken from 10 healthy volunteers. Samples were labeled with a panel of monoclonal antibodies specific to CD3, alpha/beta TCR and gamma/delta TCR in avidin-biotin-peroxidase complex (ABC) staining. Lymphocytes expressing gamma/delta TCRs were very low in non-lesional mucosa and clinically healthy mucosa. By contrast, gamma/delta T-cells were numerous and observed in all RAU lesions especially within the epithelium, inflammatory infiltrates and at perivascular locations. The count of gamma/delta T-cells was high in connective tissue of RAU (200 +/- 126 cells/mm2) compared with connective tissue of controls (4+/-4 cells/mm2; P<0.0001) or non-lesional mucosa (5+/-7 cells/mm2). Interestingly, the density of gamma/delta T-cells was also high in the epithelium of RAU (70+/-34 cells/mm2) compared with the epithelium of non-lesional mucosa (2.8+/-06 cells/mm2; P<0.0001) or epithelium of healthy controls (1.2+/-1.5 cells/mm2; P<0.0001). Moreover, the mean percentage of gamma/delta+ T-cells among total CD3+ lymphocytes was increased in the connective tissue area from 4% and 5% in controls and non-lesional mucosa, respectively, to 19% in RAU. In epithelial areas, the average percentage was increased from 2% and 6% in controls and non-lesional mucosa, respectively, to 36% in RAU. These data showed that gamma/delta T-cells are more numerous in RAU lesions and such an increase was purely restricted to RAU inflammatory areas.

Authors+Show Affiliations

Department of Medicine, Helsinki University Central Hospital, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11071244

Citation

Natah, S S., et al. "Increased Density of Lymphocytes Bearing Gamma/delta T-cell Receptors in Recurrent Aphthous Ulceration (RAU)." International Journal of Oral and Maxillofacial Surgery, vol. 29, no. 5, 2000, pp. 375-80.
Natah SS, Häyrinen-Immonen R, Hietanen J, et al. Increased density of lymphocytes bearing gamma/delta T-cell receptors in recurrent aphthous ulceration (RAU). Int J Oral Maxillofac Surg. 2000;29(5):375-80.
Natah, S. S., Häyrinen-Immonen, R., Hietanen, J., Patinen, P., Malmström, M., Savilahti, E., & Konttinen, Y. T. (2000). Increased density of lymphocytes bearing gamma/delta T-cell receptors in recurrent aphthous ulceration (RAU). International Journal of Oral and Maxillofacial Surgery, 29(5), 375-80.
Natah SS, et al. Increased Density of Lymphocytes Bearing Gamma/delta T-cell Receptors in Recurrent Aphthous Ulceration (RAU). Int J Oral Maxillofac Surg. 2000;29(5):375-80. PubMed PMID: 11071244.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased density of lymphocytes bearing gamma/delta T-cell receptors in recurrent aphthous ulceration (RAU). AU - Natah,S S, AU - Häyrinen-Immonen,R, AU - Hietanen,J, AU - Patinen,P, AU - Malmström,M, AU - Savilahti,E, AU - Konttinen,Y T, PY - 2000/11/9/pubmed PY - 2001/3/3/medline PY - 2000/11/9/entrez SP - 375 EP - 80 JF - International journal of oral and maxillofacial surgery JO - Int J Oral Maxillofac Surg VL - 29 IS - 5 N2 - Lymphocytes bearing the T-cell receptor (TCR) gamma/delta are increased in the peripheral blood of patients with recurrent aphthous ulcers (RAU) and Behcet's disease. In this study, we examined whether the density of TCR-gamma/delta bearing lymphocytes was also increased locally in RAU lesions. Ten RAU lesions from ten patients were compared with ulcer-free mucosa from sites contralateral to the lesions, and with 10 samples of clinically healthy oral mucosa taken from 10 healthy volunteers. Samples were labeled with a panel of monoclonal antibodies specific to CD3, alpha/beta TCR and gamma/delta TCR in avidin-biotin-peroxidase complex (ABC) staining. Lymphocytes expressing gamma/delta TCRs were very low in non-lesional mucosa and clinically healthy mucosa. By contrast, gamma/delta T-cells were numerous and observed in all RAU lesions especially within the epithelium, inflammatory infiltrates and at perivascular locations. The count of gamma/delta T-cells was high in connective tissue of RAU (200 +/- 126 cells/mm2) compared with connective tissue of controls (4+/-4 cells/mm2; P<0.0001) or non-lesional mucosa (5+/-7 cells/mm2). Interestingly, the density of gamma/delta T-cells was also high in the epithelium of RAU (70+/-34 cells/mm2) compared with the epithelium of non-lesional mucosa (2.8+/-06 cells/mm2; P<0.0001) or epithelium of healthy controls (1.2+/-1.5 cells/mm2; P<0.0001). Moreover, the mean percentage of gamma/delta+ T-cells among total CD3+ lymphocytes was increased in the connective tissue area from 4% and 5% in controls and non-lesional mucosa, respectively, to 19% in RAU. In epithelial areas, the average percentage was increased from 2% and 6% in controls and non-lesional mucosa, respectively, to 36% in RAU. These data showed that gamma/delta T-cells are more numerous in RAU lesions and such an increase was purely restricted to RAU inflammatory areas. SN - 0901-5027 UR - https://www.unboundmedicine.com/medline/citation/11071244/Increased_density_of_lymphocytes_bearing_gamma/delta_T_cell_receptors_in_recurrent_aphthous_ulceration__RAU__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0901-5027(00)80057-0 DB - PRIME DP - Unbound Medicine ER -