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Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies.
Br J Cancer. 2000 Dec; 83(11):1488-94.BJ

Abstract

Human epithelial ovarian carcinoma is well-known as a sex steroid-dependent neoplasm, but the possible biological significance of progesterone receptor (PR) in this cancer remains controversial. Recently, two isoforms of human PR, PRA and PRB, have been characterized and different functional characteristics have been reported for these two isoforms. We therefore examined immunohistochemistry (107 cases) and reverse transcription-polymerase chain reaction (RT-PCR) (16 cases) for PRA, PRB, and oestrogen receptor-a (ER-a). Labeling indices (LI) for PRA and PRB were 2.4 and 43.6, respectively, and the difference was statistically significant. PRB LI, but not PRA LI, as well as performance status, stage, and residual tumour turned out to be independent prognostic factors following multivariate analysis. There was also a significant correlation between ER-a LI and PRB LI (r = 0.595, P < 0.0001), suggestive of a possible interaction between these two receptors. RT-PCR also detected the expression of PR isoform transcripts in the same pattern as was observed with immunohistochemistry. Results of these studies indicate that PRA and PRB both mediate distinct pathways of progesterone action in ovarian carcinoma. Moreover, it is important to examine PRB LI as a prognostic factor in the cases of human epithelial ovarian carcinoma.

Authors+Show Affiliations

Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11076658

Citation

Akahira, J, et al. "Progesterone Receptor Isoforms a and B in Human Epithelial Ovarian Carcinoma: Immunohistochemical and RT-PCR Studies." British Journal of Cancer, vol. 83, no. 11, 2000, pp. 1488-94.
Akahira J, Inoue T, Suzuki T, et al. Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies. Br J Cancer. 2000;83(11):1488-94.
Akahira, J., Inoue, T., Suzuki, T., Ito, K., Konno, R., Sato, S., Moriya, T., Okamura, K., Yajima, A., & Sasano, H. (2000). Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies. British Journal of Cancer, 83(11), 1488-94.
Akahira J, et al. Progesterone Receptor Isoforms a and B in Human Epithelial Ovarian Carcinoma: Immunohistochemical and RT-PCR Studies. Br J Cancer. 2000;83(11):1488-94. PubMed PMID: 11076658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies. AU - Akahira,J, AU - Inoue,T, AU - Suzuki,T, AU - Ito,K, AU - Konno,R, AU - Sato,S, AU - Moriya,T, AU - Okamura,K, AU - Yajima,A, AU - Sasano,H, PY - 2000/11/15/pubmed PY - 2001/2/28/medline PY - 2000/11/15/entrez SP - 1488 EP - 94 JF - British journal of cancer JO - Br J Cancer VL - 83 IS - 11 N2 - Human epithelial ovarian carcinoma is well-known as a sex steroid-dependent neoplasm, but the possible biological significance of progesterone receptor (PR) in this cancer remains controversial. Recently, two isoforms of human PR, PRA and PRB, have been characterized and different functional characteristics have been reported for these two isoforms. We therefore examined immunohistochemistry (107 cases) and reverse transcription-polymerase chain reaction (RT-PCR) (16 cases) for PRA, PRB, and oestrogen receptor-a (ER-a). Labeling indices (LI) for PRA and PRB were 2.4 and 43.6, respectively, and the difference was statistically significant. PRB LI, but not PRA LI, as well as performance status, stage, and residual tumour turned out to be independent prognostic factors following multivariate analysis. There was also a significant correlation between ER-a LI and PRB LI (r = 0.595, P < 0.0001), suggestive of a possible interaction between these two receptors. RT-PCR also detected the expression of PR isoform transcripts in the same pattern as was observed with immunohistochemistry. Results of these studies indicate that PRA and PRB both mediate distinct pathways of progesterone action in ovarian carcinoma. Moreover, it is important to examine PRB LI as a prognostic factor in the cases of human epithelial ovarian carcinoma. SN - 0007-0920 UR - https://www.unboundmedicine.com/medline/citation/11076658/Progesterone_receptor_isoforms_A_and_B_in_human_epithelial_ovarian_carcinoma:_immunohistochemical_and_RT_PCR_studies_ L2 - https://doi.org/10.1054/bjoc.2000.1463 DB - PRIME DP - Unbound Medicine ER -