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Increased expression of major basic protein (MBP) and interleukin-5(IL-5) in middle ear biopsy specimens from atopic patients with persistent otitis media with effusion.
Otolaryngol Head Neck Surg. 2000 Nov; 123(5):533-8.OH

Abstract

BACKGROUND

Molecular biologic evidence to support an etiologic role for allergy in the pathogenesis of persistent otitis media with effusion (OME) is lacking.

OBJECTIVE

The goal of this article was to document expression of allergy-associated Th-2-type cytokines and inflammatory cells in the middle ear mucosa of children with persistent OME.

METHODS

With immunocytochemistry (CD3, major basic protein) and in situ hybridization (interleukin-5 mRNA), middle ear biopsy specimens from 7 children with persistent OME were stained. Nonatopic stapedectomy patients with no history of otitis media served as controls (n = 7).

RESULTS

There was a statistically significant (P< 0.05) difference in expression of CD3, major basic protein, and interleukin-5 between experimental and control subjects. All 8 OME patients proved to be atopic by ELISA testing.

CONCLUSIONS

Type I allergy involving a Th-2-type cytokine and cellular profile may be a contributing factor in the persistence of OME in atopic children.

SIGNIFICANCE

The middle ear may serve as a target organ for allergic inflammation, suggesting that appropriate allergy management may be a useful adjunct to the management of OME.

Authors+Show Affiliations

Meakins-Christie Laboratories, and the Department of Otolaryngology, McGill University, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11077335

Citation

Wright, E D., et al. "Increased Expression of Major Basic Protein (MBP) and interleukin-5(IL-5) in Middle Ear Biopsy Specimens From Atopic Patients With Persistent Otitis Media With Effusion." Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery, vol. 123, no. 5, 2000, pp. 533-8.
Wright ED, Hurst D, Miotto D, et al. Increased expression of major basic protein (MBP) and interleukin-5(IL-5) in middle ear biopsy specimens from atopic patients with persistent otitis media with effusion. Otolaryngol Head Neck Surg. 2000;123(5):533-8.
Wright, E. D., Hurst, D., Miotto, D., Giguere, C., & Hamid, Q. (2000). Increased expression of major basic protein (MBP) and interleukin-5(IL-5) in middle ear biopsy specimens from atopic patients with persistent otitis media with effusion. Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery, 123(5), 533-8.
Wright ED, et al. Increased Expression of Major Basic Protein (MBP) and interleukin-5(IL-5) in Middle Ear Biopsy Specimens From Atopic Patients With Persistent Otitis Media With Effusion. Otolaryngol Head Neck Surg. 2000;123(5):533-8. PubMed PMID: 11077335.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased expression of major basic protein (MBP) and interleukin-5(IL-5) in middle ear biopsy specimens from atopic patients with persistent otitis media with effusion. AU - Wright,E D, AU - Hurst,D, AU - Miotto,D, AU - Giguere,C, AU - Hamid,Q, PY - 2000/11/15/pubmed PY - 2001/2/28/medline PY - 2000/11/15/entrez SP - 533 EP - 8 JF - Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery JO - Otolaryngol Head Neck Surg VL - 123 IS - 5 N2 - BACKGROUND: Molecular biologic evidence to support an etiologic role for allergy in the pathogenesis of persistent otitis media with effusion (OME) is lacking. OBJECTIVE: The goal of this article was to document expression of allergy-associated Th-2-type cytokines and inflammatory cells in the middle ear mucosa of children with persistent OME. METHODS: With immunocytochemistry (CD3, major basic protein) and in situ hybridization (interleukin-5 mRNA), middle ear biopsy specimens from 7 children with persistent OME were stained. Nonatopic stapedectomy patients with no history of otitis media served as controls (n = 7). RESULTS: There was a statistically significant (P< 0.05) difference in expression of CD3, major basic protein, and interleukin-5 between experimental and control subjects. All 8 OME patients proved to be atopic by ELISA testing. CONCLUSIONS: Type I allergy involving a Th-2-type cytokine and cellular profile may be a contributing factor in the persistence of OME in atopic children. SIGNIFICANCE: The middle ear may serve as a target organ for allergic inflammation, suggesting that appropriate allergy management may be a useful adjunct to the management of OME. SN - 0194-5998 UR - https://www.unboundmedicine.com/medline/citation/11077335/Increased_expression_of_major_basic_protein__MBP__and_interleukin_5_IL_5__in_middle_ear_biopsy_specimens_from_atopic_patients_with_persistent_otitis_media_with_effusion_ L2 - https://journals.sagepub.com/doi/10.1067/mhn.2000.109472?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -