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Virulent Treponema pallidum 47 kDa antigen regulates the expression of cell adhesion molecules and binding of T-lymphocytes to cultured human dermal microvascular endothelial cells.
Yonsei Med J 2000; 41(5):623-33YM

Abstract

Perivasculitis and endothelial cell abnormalities are prominent histopathologic features of syphilis. Various cutaneous lesions are the main clinical features of syphilis. We examined whether Treponema pallidum 47 kDa antigen regulates the expression of cell adhesion molecules on human dermal microvascular endothelial cells (HDMEC) and the regulation of T-lymphocytes binding to HDMEC. Using immunofluorescence flow cytometry and enzyme-linked immunosorbent assay (ELISA), we demonstrated that T. pallidum upregulated the expression of adhesion molecules, including intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and E-selectin. The 47 kDa antigen of T. pallidum also activated endothelium as measured by the upregulation of the expression of adhesion molecules on HDMEC, and it also promoted an increased adherence of T-lymphocytes to HDMEC. The expressions of ICAM-1 and VCAM-1 on HDMEC and the adherence of T-lymphocytes to HDMEC were inhibited by treatment with anti-TNF-alpha antibody or anti-IL-1alpha antibody. These results show that T. pallidum or T. pallidum-specific 47 kDa antigen are capable of stimulating HDMEC to increase the expression of ICAM-1, VCAM-1 and E-selectin and thereby, promote the adherence of T-lymphocytes. The whole process may play an important role in the immunopathogenesis of syphilis and it is likely that TNF-alpha and IL-1alpha are involved.

Authors+Show Affiliations

Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea. kwanglee@yumc.yonsei.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11079623

Citation

Lee, K H., et al. "Virulent Treponema Pallidum 47 kDa Antigen Regulates the Expression of Cell Adhesion Molecules and Binding of T-lymphocytes to Cultured Human Dermal Microvascular Endothelial Cells." Yonsei Medical Journal, vol. 41, no. 5, 2000, pp. 623-33.
Lee KH, Choi HJ, Lee MG, et al. Virulent Treponema pallidum 47 kDa antigen regulates the expression of cell adhesion molecules and binding of T-lymphocytes to cultured human dermal microvascular endothelial cells. Yonsei Med J. 2000;41(5):623-33.
Lee, K. H., Choi, H. J., Lee, M. G., & Lee, J. B. (2000). Virulent Treponema pallidum 47 kDa antigen regulates the expression of cell adhesion molecules and binding of T-lymphocytes to cultured human dermal microvascular endothelial cells. Yonsei Medical Journal, 41(5), pp. 623-33.
Lee KH, et al. Virulent Treponema Pallidum 47 kDa Antigen Regulates the Expression of Cell Adhesion Molecules and Binding of T-lymphocytes to Cultured Human Dermal Microvascular Endothelial Cells. Yonsei Med J. 2000;41(5):623-33. PubMed PMID: 11079623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virulent Treponema pallidum 47 kDa antigen regulates the expression of cell adhesion molecules and binding of T-lymphocytes to cultured human dermal microvascular endothelial cells. AU - Lee,K H, AU - Choi,H J, AU - Lee,M G, AU - Lee,J B, PY - 2000/11/18/pubmed PY - 2001/2/28/medline PY - 2000/11/18/entrez SP - 623 EP - 33 JF - Yonsei medical journal JO - Yonsei Med. J. VL - 41 IS - 5 N2 - Perivasculitis and endothelial cell abnormalities are prominent histopathologic features of syphilis. Various cutaneous lesions are the main clinical features of syphilis. We examined whether Treponema pallidum 47 kDa antigen regulates the expression of cell adhesion molecules on human dermal microvascular endothelial cells (HDMEC) and the regulation of T-lymphocytes binding to HDMEC. Using immunofluorescence flow cytometry and enzyme-linked immunosorbent assay (ELISA), we demonstrated that T. pallidum upregulated the expression of adhesion molecules, including intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and E-selectin. The 47 kDa antigen of T. pallidum also activated endothelium as measured by the upregulation of the expression of adhesion molecules on HDMEC, and it also promoted an increased adherence of T-lymphocytes to HDMEC. The expressions of ICAM-1 and VCAM-1 on HDMEC and the adherence of T-lymphocytes to HDMEC were inhibited by treatment with anti-TNF-alpha antibody or anti-IL-1alpha antibody. These results show that T. pallidum or T. pallidum-specific 47 kDa antigen are capable of stimulating HDMEC to increase the expression of ICAM-1, VCAM-1 and E-selectin and thereby, promote the adherence of T-lymphocytes. The whole process may play an important role in the immunopathogenesis of syphilis and it is likely that TNF-alpha and IL-1alpha are involved. SN - 0513-5796 UR - https://www.unboundmedicine.com/medline/citation/11079623/Virulent_Treponema_pallidum_47_kDa_antigen_regulates_the_expression_of_cell_adhesion_molecules_and_binding_of_T_lymphocytes_to_cultured_human_dermal_microvascular_endothelial_cells_ L2 - https://www.eymj.org/DOIx.php?id=10.3349/ymj.2000.41.5.623 DB - PRIME DP - Unbound Medicine ER -