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Polypyrimidine tract-binding protein inhibits translation of bip mRNA.
J Mol Biol. 2000 Nov 24; 304(2):119-33.JM

Abstract

Translation initiation of human Bip mRNA is directed by an internal ribosomal entry site (IRES) located in the 5' non-translated region. No trans-acting factor possibly involved in this process has as of yet been identified. For the encephalomyocarditis virus and other picornaviruses, polypyrimidine tract-binding protein (PTB) has been found to enhance the translation through IRES elements, probably by interaction with the IRES structure. Here, we report that PTB specifically binds to the central region (nt 50-117) of the Bip 5' non-translated region. Addition of purified PTB to rabbit reticulocyte lysate and overexpression of PTB in Cos-7 cells selectively inhibited Bip IRES-dependent translation. On the other hand, depletion of endogenous PTB or addition of an RNA interacting with PTB enhanced the translational initiation directed by Bip IRES. These suggest that PTB can either enhance or inhibit IRES-dependent translation depending on mRNAs.

Authors+Show Affiliations

NRL, Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San31, Pohang, Hyoja-Dong, 790-784, Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11080450

Citation

Kim, Y K., et al. "Polypyrimidine Tract-binding Protein Inhibits Translation of Bip MRNA." Journal of Molecular Biology, vol. 304, no. 2, 2000, pp. 119-33.
Kim YK, Hahm B, Jang SK. Polypyrimidine tract-binding protein inhibits translation of bip mRNA. J Mol Biol. 2000;304(2):119-33.
Kim, Y. K., Hahm, B., & Jang, S. K. (2000). Polypyrimidine tract-binding protein inhibits translation of bip mRNA. Journal of Molecular Biology, 304(2), 119-33.
Kim YK, Hahm B, Jang SK. Polypyrimidine Tract-binding Protein Inhibits Translation of Bip MRNA. J Mol Biol. 2000 Nov 24;304(2):119-33. PubMed PMID: 11080450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polypyrimidine tract-binding protein inhibits translation of bip mRNA. AU - Kim,Y K, AU - Hahm,B, AU - Jang,S K, PY - 2000/11/18/pubmed PY - 2001/2/28/medline PY - 2000/11/18/entrez SP - 119 EP - 33 JF - Journal of molecular biology JO - J Mol Biol VL - 304 IS - 2 N2 - Translation initiation of human Bip mRNA is directed by an internal ribosomal entry site (IRES) located in the 5' non-translated region. No trans-acting factor possibly involved in this process has as of yet been identified. For the encephalomyocarditis virus and other picornaviruses, polypyrimidine tract-binding protein (PTB) has been found to enhance the translation through IRES elements, probably by interaction with the IRES structure. Here, we report that PTB specifically binds to the central region (nt 50-117) of the Bip 5' non-translated region. Addition of purified PTB to rabbit reticulocyte lysate and overexpression of PTB in Cos-7 cells selectively inhibited Bip IRES-dependent translation. On the other hand, depletion of endogenous PTB or addition of an RNA interacting with PTB enhanced the translational initiation directed by Bip IRES. These suggest that PTB can either enhance or inhibit IRES-dependent translation depending on mRNAs. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/11080450/Polypyrimidine_tract_binding_protein_inhibits_translation_of_bip_mRNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(00)94179-2 DB - PRIME DP - Unbound Medicine ER -