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Determination of free concentrations of ropivacaine and bupivacaine in plasma from neonates using small-scale equilibrium-dialysis followed by liquid chromatography-mass spectrometry.
J Chromatogr B Biomed Sci Appl. 2000 Oct 10; 748(2):321-30.JC

Abstract

Attempts to determine a safe plasma concentration of ropivacaine and bupivacaine in neonates have not been consistent. This might be due to an underestimation of free drug in small plasma samples by currently used techniques, e.g., ultrafiltration. We describe a simple microscale equilibrium-dialysis technique for the separation of free and bound ropivacaine and bupivacaine. The free drug in the dialysate was determined using solid-phase extraction and liquid chromatography with mass spectrometry. Pentycaine was used as an internal standard and added to the dialysates prior to extraction. The method is very selective and sensitive, as no compounds other than the analyte and internal standard were observed in the resulting chromatograms at low ng/ml levels. The limit of quantitation was 2.5 ng/ml. The calibration curve was linear in the range of 2 to 1000 ng/ml. The precision of the whole procedure was 8.1% (n=10) and 6.5% (n=7) for ropivacaine and bupivacaine, respectively. The method was tested in the analysis of plasma samples taken from neonates who had received epidural injections.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, Strathclyde Institute of Biomedical Sciences, Glasgow, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11087074

Citation

Stumpe, M, et al. "Determination of Free Concentrations of Ropivacaine and Bupivacaine in Plasma From Neonates Using Small-scale Equilibrium-dialysis Followed By Liquid Chromatography-mass Spectrometry." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 748, no. 2, 2000, pp. 321-30.
Stumpe M, Morton NS, Watson DG. Determination of free concentrations of ropivacaine and bupivacaine in plasma from neonates using small-scale equilibrium-dialysis followed by liquid chromatography-mass spectrometry. J Chromatogr B Biomed Sci Appl. 2000;748(2):321-30.
Stumpe, M., Morton, N. S., & Watson, D. G. (2000). Determination of free concentrations of ropivacaine and bupivacaine in plasma from neonates using small-scale equilibrium-dialysis followed by liquid chromatography-mass spectrometry. Journal of Chromatography. B, Biomedical Sciences and Applications, 748(2), 321-30.
Stumpe M, Morton NS, Watson DG. Determination of Free Concentrations of Ropivacaine and Bupivacaine in Plasma From Neonates Using Small-scale Equilibrium-dialysis Followed By Liquid Chromatography-mass Spectrometry. J Chromatogr B Biomed Sci Appl. 2000 Oct 10;748(2):321-30. PubMed PMID: 11087074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of free concentrations of ropivacaine and bupivacaine in plasma from neonates using small-scale equilibrium-dialysis followed by liquid chromatography-mass spectrometry. AU - Stumpe,M, AU - Morton,N S, AU - Watson,D G, PY - 2000/11/22/pubmed PY - 2001/3/7/medline PY - 2000/11/22/entrez SP - 321 EP - 30 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J Chromatogr B Biomed Sci Appl VL - 748 IS - 2 N2 - Attempts to determine a safe plasma concentration of ropivacaine and bupivacaine in neonates have not been consistent. This might be due to an underestimation of free drug in small plasma samples by currently used techniques, e.g., ultrafiltration. We describe a simple microscale equilibrium-dialysis technique for the separation of free and bound ropivacaine and bupivacaine. The free drug in the dialysate was determined using solid-phase extraction and liquid chromatography with mass spectrometry. Pentycaine was used as an internal standard and added to the dialysates prior to extraction. The method is very selective and sensitive, as no compounds other than the analyte and internal standard were observed in the resulting chromatograms at low ng/ml levels. The limit of quantitation was 2.5 ng/ml. The calibration curve was linear in the range of 2 to 1000 ng/ml. The precision of the whole procedure was 8.1% (n=10) and 6.5% (n=7) for ropivacaine and bupivacaine, respectively. The method was tested in the analysis of plasma samples taken from neonates who had received epidural injections. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/11087074/Determination_of_free_concentrations_of_ropivacaine_and_bupivacaine_in_plasma_from_neonates_using_small_scale_equilibrium_dialysis_followed_by_liquid_chromatography_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -