Tags

Type your tag names separated by a space and hit enter

Partial characterization of peroxidase and polyphenol oxidase activities in blackberry fruits.
J Agric Food Chem 2000; 48(11):5459-64JA

Abstract

A partial characterization of peroxidase (POD) and polyphenol oxidase (PPO) activities in blackberry fruits is described. Two cultivars of blackberry (Wild and Thornless) were analyzed for POD and PPO activities. Stable and highly active POD and PPO extracts were obtained using insoluble poly(vinylpyrrolidone) and Triton X-100 in 0.05 M sodium phosphate, pH 7.5, buffer. Blackberry POD and PPO activities have a pH optimum of 6.5, in a reaction mixture of 0.2 M sodium phosphate. Optimal POD activity was found with 3% o-dianisidine. Maximum PPO activity was found with catechol (catecholase activity) followed by 4-methylcatechol. Polyacrylamide gel electrophoresis of blackberry extracts under non-denaturing conditions resolved in various bands. In the POD extracts of Wild fruits, there was only one band with a mobility of 0.12. In the Thornless POD extracts there were three well-resolved bands, with R(f) values of 0.63, 0.36, and 0.09. Both the Wild and Thornless blackberry cultivars produced a single band of PPO, with R(f) values of 0.1 for Wild and 0.06 for Thornless.

Authors+Show Affiliations

Plant Foods Science and Technology Department, Instituto del Frío (CSIC), Ciudad Universitaria, 28040 Madrid, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11087502

Citation

González, E M., et al. "Partial Characterization of Peroxidase and Polyphenol Oxidase Activities in Blackberry Fruits." Journal of Agricultural and Food Chemistry, vol. 48, no. 11, 2000, pp. 5459-64.
González EM, de Ancos B, Cano MP. Partial characterization of peroxidase and polyphenol oxidase activities in blackberry fruits. J Agric Food Chem. 2000;48(11):5459-64.
González, E. M., de Ancos, B., & Cano, M. P. (2000). Partial characterization of peroxidase and polyphenol oxidase activities in blackberry fruits. Journal of Agricultural and Food Chemistry, 48(11), pp. 5459-64.
González EM, de Ancos B, Cano MP. Partial Characterization of Peroxidase and Polyphenol Oxidase Activities in Blackberry Fruits. J Agric Food Chem. 2000;48(11):5459-64. PubMed PMID: 11087502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Partial characterization of peroxidase and polyphenol oxidase activities in blackberry fruits. AU - González,E M, AU - de Ancos,B, AU - Cano,M P, PY - 2000/11/23/pubmed PY - 2001/2/28/medline PY - 2000/11/23/entrez SP - 5459 EP - 64 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 48 IS - 11 N2 - A partial characterization of peroxidase (POD) and polyphenol oxidase (PPO) activities in blackberry fruits is described. Two cultivars of blackberry (Wild and Thornless) were analyzed for POD and PPO activities. Stable and highly active POD and PPO extracts were obtained using insoluble poly(vinylpyrrolidone) and Triton X-100 in 0.05 M sodium phosphate, pH 7.5, buffer. Blackberry POD and PPO activities have a pH optimum of 6.5, in a reaction mixture of 0.2 M sodium phosphate. Optimal POD activity was found with 3% o-dianisidine. Maximum PPO activity was found with catechol (catecholase activity) followed by 4-methylcatechol. Polyacrylamide gel electrophoresis of blackberry extracts under non-denaturing conditions resolved in various bands. In the POD extracts of Wild fruits, there was only one band with a mobility of 0.12. In the Thornless POD extracts there were three well-resolved bands, with R(f) values of 0.63, 0.36, and 0.09. Both the Wild and Thornless blackberry cultivars produced a single band of PPO, with R(f) values of 0.1 for Wild and 0.06 for Thornless. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/11087502/Partial_characterization_of_peroxidase_and_polyphenol_oxidase_activities_in_blackberry_fruits_ L2 - https://dx.doi.org/10.1021/jf000169w DB - PRIME DP - Unbound Medicine ER -