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Embryonic stem cells and transgenic mice ubiquitously expressing a tau-tagged green fluorescent protein.
Dev Biol. 2000 Dec 01; 228(1):19-28.DB

Abstract

We have generated embryonic stem (ES) cells and transgenic mice carrying a tau-tagged green fluorescent protein (GFP) transgene under the control of a powerful promoter active in all cell types including those of the central nervous system. GFP requires no substrate and can be detected in fixed or living cells so is an attractive genetic marker. Tau-tagged GFP labels subcellular structures, including axons and the mitotic machinery, by binding the GFP to microtubules. This allows cell morphology to be visualized in exquisite detail. We test the application of cells derived from these mice in several types of cell-mixing experiments and demonstrate that the morphology of tau-GFP-expressing cells can be readily visualized after they have integrated into unlabeled host cells or tissues. We anticipate that these ES cells and transgenic mice will prove a novel and powerful tool for a wide variety of applications including the development of neural transplantation technologies in animal models and fundamental research into axon pathfinding mechanisms. A major advantage of the tau-GFP label is that it can be detected in living cells and labeled cells and their processes can be identified and subjected to a variety of manipulations such as electrophysiological cell recording.

Authors+Show Affiliations

Department of Biomedical Sciences, Centre for Developmental Biology, The University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, EH8 9XD, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11087623

Citation

Pratt, T, et al. "Embryonic Stem Cells and Transgenic Mice Ubiquitously Expressing a Tau-tagged Green Fluorescent Protein." Developmental Biology, vol. 228, no. 1, 2000, pp. 19-28.
Pratt T, Sharp L, Nichols J, et al. Embryonic stem cells and transgenic mice ubiquitously expressing a tau-tagged green fluorescent protein. Dev Biol. 2000;228(1):19-28.
Pratt, T., Sharp, L., Nichols, J., Price, D. J., & Mason, J. O. (2000). Embryonic stem cells and transgenic mice ubiquitously expressing a tau-tagged green fluorescent protein. Developmental Biology, 228(1), 19-28.
Pratt T, et al. Embryonic Stem Cells and Transgenic Mice Ubiquitously Expressing a Tau-tagged Green Fluorescent Protein. Dev Biol. 2000 Dec 1;228(1):19-28. PubMed PMID: 11087623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Embryonic stem cells and transgenic mice ubiquitously expressing a tau-tagged green fluorescent protein. AU - Pratt,T, AU - Sharp,L, AU - Nichols,J, AU - Price,D J, AU - Mason,J O, PY - 2000/11/23/pubmed PY - 2001/2/28/medline PY - 2000/11/23/entrez SP - 19 EP - 28 JF - Developmental biology JO - Dev. Biol. VL - 228 IS - 1 N2 - We have generated embryonic stem (ES) cells and transgenic mice carrying a tau-tagged green fluorescent protein (GFP) transgene under the control of a powerful promoter active in all cell types including those of the central nervous system. GFP requires no substrate and can be detected in fixed or living cells so is an attractive genetic marker. Tau-tagged GFP labels subcellular structures, including axons and the mitotic machinery, by binding the GFP to microtubules. This allows cell morphology to be visualized in exquisite detail. We test the application of cells derived from these mice in several types of cell-mixing experiments and demonstrate that the morphology of tau-GFP-expressing cells can be readily visualized after they have integrated into unlabeled host cells or tissues. We anticipate that these ES cells and transgenic mice will prove a novel and powerful tool for a wide variety of applications including the development of neural transplantation technologies in animal models and fundamental research into axon pathfinding mechanisms. A major advantage of the tau-GFP label is that it can be detected in living cells and labeled cells and their processes can be identified and subjected to a variety of manipulations such as electrophysiological cell recording. SN - 0012-1606 UR - https://www.unboundmedicine.com/medline/citation/11087623/Embryonic_stem_cells_and_transgenic_mice_ubiquitously_expressing_a_tau_tagged_green_fluorescent_protein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(00)99935-4 DB - PRIME DP - Unbound Medicine ER -