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Methylenetetrahydrofolate reductase mutation (677C-->T) negatively influences plasma homocysteine response to marginal folate intake in elderly women.
Metabolism 2000; 49(11):1440-3M

Abstract

Individuals who are homozygous for the methylenetetrahydrofolate reductase (MTHFR) 677C --> T mutation have depressed serum folate (SF) and elevated plasma total homocysteine (tHcy) concentrations, which may affect folate requirements and increase the risk for coronary artery disease. A controlled metabolic study (14 weeks) using a depletion/repletion protocol was performed in women (aged 60 to 85 years, N = 33) to provide age-specific data on the effects of the MTHFR mutation on SF and tHcy status. Subjects consumed a moderately folate-deplete diet (118 microg/d) for 7 weeks, followed by 7 weeks of folate repletion with 200 or 415 microg/d provided as two different treatments. Following folate depletion, the mean SF concentration was lower for homozygous (P = .017) versus heterozygous subjects. Homozygotes for the 677C --> T mutation showed a higher (P = .015) percent increase in plasma tHcy (44%) than heterozygous (20%) or normal (15%) subjects. At week 7, the mean plasma tHcy concentration was higher in homozygous subjects (12.5 +/- 5.3 micromol/L, mean +/- SD) versus the heterozygous (10.8 +/- 3.8 micromol/L, P = .008) or normal (11.3 +/- 2.7 micromol/L, P = .001) genotype groups. Following folate repletion, plasma tHcy concentrations were not different between genotype groups, despite a higher (P < .016) SF concentration in subjects with the homozygous genotype. These data suggest that older women who are homozygous for the MTHFR 677C --> T mutation may be at risk for greater elevations in plasma tHcy in response to moderately low folate intake as compared with individuals with the normal or heterozygous genotypes.

Authors+Show Affiliations

Food Science and Human Nutrition Department, College of Agricultural and Life Sciences, University of Florida, Gainesville 32611-0370, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11092508

Citation

Kauwell, G P., et al. "Methylenetetrahydrofolate Reductase Mutation (677C-->T) Negatively Influences Plasma Homocysteine Response to Marginal Folate Intake in Elderly Women." Metabolism: Clinical and Experimental, vol. 49, no. 11, 2000, pp. 1440-3.
Kauwell GP, Wilsky CE, Cerda JJ, et al. Methylenetetrahydrofolate reductase mutation (677C-->T) negatively influences plasma homocysteine response to marginal folate intake in elderly women. Metab Clin Exp. 2000;49(11):1440-3.
Kauwell, G. P., Wilsky, C. E., Cerda, J. J., Herrlinger-Garcia, K., Hutson, A. D., Theriaque, D. W., ... Bailey, L. B. (2000). Methylenetetrahydrofolate reductase mutation (677C-->T) negatively influences plasma homocysteine response to marginal folate intake in elderly women. Metabolism: Clinical and Experimental, 49(11), pp. 1440-3.
Kauwell GP, et al. Methylenetetrahydrofolate Reductase Mutation (677C-->T) Negatively Influences Plasma Homocysteine Response to Marginal Folate Intake in Elderly Women. Metab Clin Exp. 2000;49(11):1440-3. PubMed PMID: 11092508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methylenetetrahydrofolate reductase mutation (677C-->T) negatively influences plasma homocysteine response to marginal folate intake in elderly women. AU - Kauwell,G P, AU - Wilsky,C E, AU - Cerda,J J, AU - Herrlinger-Garcia,K, AU - Hutson,A D, AU - Theriaque,D W, AU - Boddie,A, AU - Rampersaud,G C, AU - Bailey,L B, PY - 2000/11/25/pubmed PY - 2001/2/28/medline PY - 2000/11/25/entrez SP - 1440 EP - 3 JF - Metabolism: clinical and experimental JO - Metab. Clin. Exp. VL - 49 IS - 11 N2 - Individuals who are homozygous for the methylenetetrahydrofolate reductase (MTHFR) 677C --> T mutation have depressed serum folate (SF) and elevated plasma total homocysteine (tHcy) concentrations, which may affect folate requirements and increase the risk for coronary artery disease. A controlled metabolic study (14 weeks) using a depletion/repletion protocol was performed in women (aged 60 to 85 years, N = 33) to provide age-specific data on the effects of the MTHFR mutation on SF and tHcy status. Subjects consumed a moderately folate-deplete diet (118 microg/d) for 7 weeks, followed by 7 weeks of folate repletion with 200 or 415 microg/d provided as two different treatments. Following folate depletion, the mean SF concentration was lower for homozygous (P = .017) versus heterozygous subjects. Homozygotes for the 677C --> T mutation showed a higher (P = .015) percent increase in plasma tHcy (44%) than heterozygous (20%) or normal (15%) subjects. At week 7, the mean plasma tHcy concentration was higher in homozygous subjects (12.5 +/- 5.3 micromol/L, mean +/- SD) versus the heterozygous (10.8 +/- 3.8 micromol/L, P = .008) or normal (11.3 +/- 2.7 micromol/L, P = .001) genotype groups. Following folate repletion, plasma tHcy concentrations were not different between genotype groups, despite a higher (P < .016) SF concentration in subjects with the homozygous genotype. These data suggest that older women who are homozygous for the MTHFR 677C --> T mutation may be at risk for greater elevations in plasma tHcy in response to moderately low folate intake as compared with individuals with the normal or heterozygous genotypes. SN - 0026-0495 UR - https://www.unboundmedicine.com/medline/citation/11092508/Methylenetetrahydrofolate_reductase_mutation__677C__>T__negatively_influences_plasma_homocysteine_response_to_marginal_folate_intake_in_elderly_women_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0026-0495(00)38227-0 DB - PRIME DP - Unbound Medicine ER -