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Nontoxigenic sorbitol-fermenting Escherichia coli O157:H- associated with a family outbreak of diarrhoea.
Wien Klin Wochenschr. 2000 Oct 13; 112(19):846-50.WK

Abstract

A recent study from Germany reported the isolation of E. coli O157:H7/H- from patients with non-bloody diarrhoea and hemolytic uremic syndrome, questioning the role of Shiga toxin as the main trait of virulence for human disease. We isolated 6 sorbitol-fermenting E. coli O157:H- strains that do not contain Shiga toxin genes. The isolates originated from an outbreak (3 patients, 3 asymptomatic contacts) of non-bloody diarrhoea affecting two families sharing one household. Two children (age 10 months and 2 years) suffered severe diarrhoea over 30 and 10 days, respectively. Their uncle had moderate diarrhoea for 2 weeks. In contrast to the other isolates, the uncle's strain (EH109) did not harbour a chromosomal eae gene encoding gamma-intimin nor the plasmid gene E-hly; it also showed a PFGE pattern that was different from the unique pattern of the other isolates. Employing PFGE, phage typing, and P-gene typing, five of the six stx negative isolates were indistinguishable from the stx 2 positive "Bavarian outbreak strain". The only human serum tested, obtained from one asymptomatic contact, contained antibodies to the O157 lipopolysaccharide antigen. Our finding of five stx negative sorbitol-fermenting E. coli O157:H- isolates (harbouring eae and E-hly) associated with an outbreak of non-bloody diarrhoea supports the hypothesis that Stx production is not obligatory for the pathogenicity of E. coli O157 for humans.

Authors+Show Affiliations

Bundesstaatliche bakteriologisch-serologische Untersuchungsanstalt und Institut für Hygiene und Sozialmedizin, Innsbruck, Austria. franz.allerberger@uibk.ac.atNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

11098536

Citation

Allerberger, F, et al. "Nontoxigenic Sorbitol-fermenting Escherichia Coli O157:H- Associated With a Family Outbreak of Diarrhoea." Wiener Klinische Wochenschrift, vol. 112, no. 19, 2000, pp. 846-50.
Allerberger F, Dierich MP, Gruber-Moesenbacher U, et al. Nontoxigenic sorbitol-fermenting Escherichia coli O157:H- associated with a family outbreak of diarrhoea. Wien Klin Wochenschr. 2000;112(19):846-50.
Allerberger, F., Dierich, M. P., Gruber-Moesenbacher, U., Liesegang, A., Prager, R., Hartmann, G., Rabsch, W., Tschäpe, H., & Karch, H. (2000). Nontoxigenic sorbitol-fermenting Escherichia coli O157:H- associated with a family outbreak of diarrhoea. Wiener Klinische Wochenschrift, 112(19), 846-50.
Allerberger F, et al. Nontoxigenic Sorbitol-fermenting Escherichia Coli O157:H- Associated With a Family Outbreak of Diarrhoea. Wien Klin Wochenschr. 2000 Oct 13;112(19):846-50. PubMed PMID: 11098536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nontoxigenic sorbitol-fermenting Escherichia coli O157:H- associated with a family outbreak of diarrhoea. AU - Allerberger,F, AU - Dierich,M P, AU - Gruber-Moesenbacher,U, AU - Liesegang,A, AU - Prager,R, AU - Hartmann,G, AU - Rabsch,W, AU - Tschäpe,H, AU - Karch,H, PY - 2000/12/1/pubmed PY - 2001/2/28/medline PY - 2000/12/1/entrez SP - 846 EP - 50 JF - Wiener klinische Wochenschrift JO - Wien Klin Wochenschr VL - 112 IS - 19 N2 - A recent study from Germany reported the isolation of E. coli O157:H7/H- from patients with non-bloody diarrhoea and hemolytic uremic syndrome, questioning the role of Shiga toxin as the main trait of virulence for human disease. We isolated 6 sorbitol-fermenting E. coli O157:H- strains that do not contain Shiga toxin genes. The isolates originated from an outbreak (3 patients, 3 asymptomatic contacts) of non-bloody diarrhoea affecting two families sharing one household. Two children (age 10 months and 2 years) suffered severe diarrhoea over 30 and 10 days, respectively. Their uncle had moderate diarrhoea for 2 weeks. In contrast to the other isolates, the uncle's strain (EH109) did not harbour a chromosomal eae gene encoding gamma-intimin nor the plasmid gene E-hly; it also showed a PFGE pattern that was different from the unique pattern of the other isolates. Employing PFGE, phage typing, and P-gene typing, five of the six stx negative isolates were indistinguishable from the stx 2 positive "Bavarian outbreak strain". The only human serum tested, obtained from one asymptomatic contact, contained antibodies to the O157 lipopolysaccharide antigen. Our finding of five stx negative sorbitol-fermenting E. coli O157:H- isolates (harbouring eae and E-hly) associated with an outbreak of non-bloody diarrhoea supports the hypothesis that Stx production is not obligatory for the pathogenicity of E. coli O157 for humans. SN - 0043-5325 UR - https://www.unboundmedicine.com/medline/citation/11098536/Nontoxigenic_sorbitol_fermenting_Escherichia_coli_O157:H__associated_with_a_family_outbreak_of_diarrhoea_ L2 - https://medlineplus.gov/ecoliinfections.html DB - PRIME DP - Unbound Medicine ER -