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Genetic heterogeneity and molecular epidemiology of GB virus C/hepatitis G virus in China.
J Hum Virol. 2000 Nov-Dec; 3(6):299-305.JH

Abstract

OBJECTIVE

The inter- and intrapatient genetic variation of GB virus C (GBV-C)/hepatitis G virus (HGV) was investigated to characterize the molecular epidemiologic profile of GBV-C/ HGV infection in China, an area endemic for viral hepatitis. The intrapatient variation of hepatitis C virus (HCV) from the same patients was compared to that of GBV-C/HGV.

STUDY DESIGN/METHODS

GB virus C/HGV RNA was amplified by polymerase chain reaction in 88 patients with hepatitis C, hepatitis B or presumed non-A-E hepatitis from three cities in China. Five clones of the GBV-C/HGV NS3 region were sequenced from each GBV-C/HGV RNA-positive patient. The corresponding region of HCV was also sequenced from patients co-infected with HCV. Representative sequences of the GBV-C/HGV NS3 region from each patient and those of isolates from other continents were subjected to phylogenetic analyses.

RESULTS

GB virus C/HGV was detected in 22 (25.25%) of 88 patients: 9 (21.4%) of 42 patients with presumed non-A-E hepatitis, 10 (27.7%) of 36 patients with hepatitis C, 3 (30.0%) in 10 patients with hepatitis B and C, and in none of 60 volunteer blood donors. The extent of nucleotide variation was less between Chinese isolates (2.4-17%; median, 10.4%) than between Chinese isolates and seven isolates from outside China (10.5-19.5%; median, 15.3%). Intrapatient sequence variation ranged from 0 to 1.75%, with a mean of 0.57 +/- 0.51%. Phylogenetic analysis grouped most Chinese isolates into four geographically specific clusters with a divergence of 10% to 16% from each other. The ratio of nonsynonymous to synonymous substitutions of GBV-C/HGV (Ka/Ks 0.019) was much lower than for HCV (0.071) in the same patients.

CONCLUSION

Chinese isolates of GBV-C/HGV are genetically distinct. There are local strains as well as shared strains between different locales. The extent of amino acid sequence conservation suggests strong selection against nonsynonymous substitutions in the GBV-C/HGV genome.

Authors+Show Affiliations

ntramural Research Support Program, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11100910

Citation

An, P, et al. "Genetic Heterogeneity and Molecular Epidemiology of GB Virus C/hepatitis G Virus in China." Journal of Human Virology, vol. 3, no. 6, 2000, pp. 299-305.
An P, Luo H, Lu T, et al. Genetic heterogeneity and molecular epidemiology of GB virus C/hepatitis G virus in China. J Hum Virol. 2000;3(6):299-305.
An, P., Luo, H., Lu, T., O'Brien, S. J., & Winkler, C. (2000). Genetic heterogeneity and molecular epidemiology of GB virus C/hepatitis G virus in China. Journal of Human Virology, 3(6), 299-305.
An P, et al. Genetic Heterogeneity and Molecular Epidemiology of GB Virus C/hepatitis G Virus in China. J Hum Virol. 2000 Nov-Dec;3(6):299-305. PubMed PMID: 11100910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic heterogeneity and molecular epidemiology of GB virus C/hepatitis G virus in China. AU - An,P, AU - Luo,H, AU - Lu,T, AU - O'Brien,S J, AU - Winkler,C, PY - 2000/1/11/pubmed PY - 2001/6/23/medline PY - 2000/1/11/entrez SP - 299 EP - 305 JF - Journal of human virology JO - J Hum Virol VL - 3 IS - 6 N2 - OBJECTIVE: The inter- and intrapatient genetic variation of GB virus C (GBV-C)/hepatitis G virus (HGV) was investigated to characterize the molecular epidemiologic profile of GBV-C/ HGV infection in China, an area endemic for viral hepatitis. The intrapatient variation of hepatitis C virus (HCV) from the same patients was compared to that of GBV-C/HGV. STUDY DESIGN/METHODS: GB virus C/HGV RNA was amplified by polymerase chain reaction in 88 patients with hepatitis C, hepatitis B or presumed non-A-E hepatitis from three cities in China. Five clones of the GBV-C/HGV NS3 region were sequenced from each GBV-C/HGV RNA-positive patient. The corresponding region of HCV was also sequenced from patients co-infected with HCV. Representative sequences of the GBV-C/HGV NS3 region from each patient and those of isolates from other continents were subjected to phylogenetic analyses. RESULTS: GB virus C/HGV was detected in 22 (25.25%) of 88 patients: 9 (21.4%) of 42 patients with presumed non-A-E hepatitis, 10 (27.7%) of 36 patients with hepatitis C, 3 (30.0%) in 10 patients with hepatitis B and C, and in none of 60 volunteer blood donors. The extent of nucleotide variation was less between Chinese isolates (2.4-17%; median, 10.4%) than between Chinese isolates and seven isolates from outside China (10.5-19.5%; median, 15.3%). Intrapatient sequence variation ranged from 0 to 1.75%, with a mean of 0.57 +/- 0.51%. Phylogenetic analysis grouped most Chinese isolates into four geographically specific clusters with a divergence of 10% to 16% from each other. The ratio of nonsynonymous to synonymous substitutions of GBV-C/HGV (Ka/Ks 0.019) was much lower than for HCV (0.071) in the same patients. CONCLUSION: Chinese isolates of GBV-C/HGV are genetically distinct. There are local strains as well as shared strains between different locales. The extent of amino acid sequence conservation suggests strong selection against nonsynonymous substitutions in the GBV-C/HGV genome. SN - 1090-9508 UR - https://www.unboundmedicine.com/medline/citation/11100910/Genetic_heterogeneity_and_molecular_epidemiology_of_GB_virus_C/hepatitis_G_virus_in_China_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11100910.ui DB - PRIME DP - Unbound Medicine ER -