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Quantitative analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay and the COBAS AMPLICOR CMV MONITOR PCR test after blood and marrow allogeneic transplantation.
J Clin Microbiol. 2000 Dec; 38(12):4356-60.JC

Abstract

The performance of a commercially available qualitative PCR test for plasma (AMPLICOR CMV Test; Roche Diagnostics) and a quantitative PCR test for plasma and leukocytes (COBAS AMPLICOR CMV MONITOR Test; Roche Diagnostics) was evaluated with samples from 50 blood or marrow allogeneic transplant recipients who received short courses of sequential ganciclovir therapy (2 weeks intravenously followed by 2 weeks orally) based on a positive cytomegalovirus (CMV) pp65 antigenemia (AG) assay. The number of persons with a positive CMV test was significantly higher for leukocyte-based assays (AG, 67.5%; PCR, 62.5%) compared to both quantitative and qualitative PCR tests of plasma (42.5 and 35%, respectively). One person developed CMV disease during the study despite a negative AG assay; in this particular case, all PCR assays were found to be positive 10 days before his death. There was a trend for earlier positivity after transplantation and more rapid negativity after initiation of ganciclovir for the tests performed on leukocytes. The mean number of CMV copies as assessed by PCR was significantly higher in leukocytes than in plasma (P = 0.02). Overall, excellent agreement (kappa coefficient, >0.75) was found only between the two PCR assays (qualitative and quantitative) based on plasma. These results suggest that either the pp65 AG assay or the COBAS AMPLICOR CMV MONITOR Test using leukocytes could be used to safely monitor CMV viremia in related allogeneic blood or marrow transplant recipients. Such a strategy will result in preemptive treatment for about two-thirds of the persons with a relatively low rate (<33%) of secondary viremic episodes following short courses of ganciclovir therapy.

Authors+Show Affiliations

Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Department of Medical Biology, St-Sacrement Hospital and Department of Medicine, Université Laval, Québec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11101564

Citation

Boivin, G, et al. "Quantitative Analysis of Cytomegalovirus (CMV) Viremia Using the Pp65 Antigenemia Assay and the COBAS AMPLICOR CMV MONITOR PCR Test After Blood and Marrow Allogeneic Transplantation." Journal of Clinical Microbiology, vol. 38, no. 12, 2000, pp. 4356-60.
Boivin G, Bélanger R, Delage R, et al. Quantitative analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay and the COBAS AMPLICOR CMV MONITOR PCR test after blood and marrow allogeneic transplantation. J Clin Microbiol. 2000;38(12):4356-60.
Boivin, G., Bélanger, R., Delage, R., Béliveau, C., Demers, C., Goyette, N., & Roy, J. (2000). Quantitative analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay and the COBAS AMPLICOR CMV MONITOR PCR test after blood and marrow allogeneic transplantation. Journal of Clinical Microbiology, 38(12), 4356-60.
Boivin G, et al. Quantitative Analysis of Cytomegalovirus (CMV) Viremia Using the Pp65 Antigenemia Assay and the COBAS AMPLICOR CMV MONITOR PCR Test After Blood and Marrow Allogeneic Transplantation. J Clin Microbiol. 2000;38(12):4356-60. PubMed PMID: 11101564.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay and the COBAS AMPLICOR CMV MONITOR PCR test after blood and marrow allogeneic transplantation. AU - Boivin,G, AU - Bélanger,R, AU - Delage,R, AU - Béliveau,C, AU - Demers,C, AU - Goyette,N, AU - Roy,J, PY - 2000/12/2/pubmed PY - 2001/2/28/medline PY - 2000/12/2/entrez SP - 4356 EP - 60 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 38 IS - 12 N2 - The performance of a commercially available qualitative PCR test for plasma (AMPLICOR CMV Test; Roche Diagnostics) and a quantitative PCR test for plasma and leukocytes (COBAS AMPLICOR CMV MONITOR Test; Roche Diagnostics) was evaluated with samples from 50 blood or marrow allogeneic transplant recipients who received short courses of sequential ganciclovir therapy (2 weeks intravenously followed by 2 weeks orally) based on a positive cytomegalovirus (CMV) pp65 antigenemia (AG) assay. The number of persons with a positive CMV test was significantly higher for leukocyte-based assays (AG, 67.5%; PCR, 62.5%) compared to both quantitative and qualitative PCR tests of plasma (42.5 and 35%, respectively). One person developed CMV disease during the study despite a negative AG assay; in this particular case, all PCR assays were found to be positive 10 days before his death. There was a trend for earlier positivity after transplantation and more rapid negativity after initiation of ganciclovir for the tests performed on leukocytes. The mean number of CMV copies as assessed by PCR was significantly higher in leukocytes than in plasma (P = 0.02). Overall, excellent agreement (kappa coefficient, >0.75) was found only between the two PCR assays (qualitative and quantitative) based on plasma. These results suggest that either the pp65 AG assay or the COBAS AMPLICOR CMV MONITOR Test using leukocytes could be used to safely monitor CMV viremia in related allogeneic blood or marrow transplant recipients. Such a strategy will result in preemptive treatment for about two-thirds of the persons with a relatively low rate (<33%) of secondary viremic episodes following short courses of ganciclovir therapy. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/11101564/Quantitative_analysis_of_cytomegalovirus__CMV__viremia_using_the_pp65_antigenemia_assay_and_the_COBAS_AMPLICOR_CMV_MONITOR_PCR_test_after_blood_and_marrow_allogeneic_transplantation_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&amp;pmid=11101564 DB - PRIME DP - Unbound Medicine ER -