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Stage and region-specific localization of lipocalin-type prostaglandin D synthase in the adult murine testis and epididymis.
J Androl. 2000 Nov-Dec; 21(6):848-54.JA

Abstract

Lipocalin-type prostaglandin D synthase in semen has been associated with male fertility, although this relationship is not well defined. To gain insight into potential mechanisms, the objective of the present study was to immunocytochemically localize lipocalin-type prostaglandin D synthase within the testis, efferent ducts, and 4 segments of mouse epididymis. In the testis, immunoperoxidase staining was localized within the Sertoli cells only at stages VI-VIII of the spermatogenic cycle, which is just prior to spermiation. Intense staining was also evident throughout the interstitial tissue, including Leydig cells. The entire epithelium of the efferent ducts, including ciliated and nonciliated cells, was immunoreactive. A distinct pattern of immunostaining for lipocalin-type prostaglandin D synthase was observed in different regions of epididymis, suggesting a possible role in sperm maturation. Staining for lipocalin-type prostaglandin D synthase was strikingly absent in the initial segment. In caput epididymidis, staining was evident throughout the cell cytoplasm of principal cells with some cells more intensely stained than adjacent ones. In the corpus region, overall staining intensity decreased and appeared to be concentrated in the apical region of principal cells, but some cells were completely unreactive. Reaction product in the cauda region was heavily concentrated on microvilli and within the epididymal lumen. In all epididymal regions, expression of lipocalin-type prostaglandin D synthase was specific to epithelial principal cells; no immunoreactivity was apparent in other cell types. The specific localization of lipocalin-type prostaglandin D synthase within the testicular interstitial tissue, Sertoli cells, and principal cells of caput epididymidis strongly suggests that this protein plays an integral role in both the development and maturation of sperm.

Authors+Show Affiliations

Department of Dairy and Animal Science, J.O. Almquist Research Center, Pennsylvania State University, University Park 16802, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11105911

Citation

Gerena, R L., et al. "Stage and Region-specific Localization of Lipocalin-type Prostaglandin D Synthase in the Adult Murine Testis and Epididymis." Journal of Andrology, vol. 21, no. 6, 2000, pp. 848-54.
Gerena RL, Eguchi N, Urade Y, et al. Stage and region-specific localization of lipocalin-type prostaglandin D synthase in the adult murine testis and epididymis. J Androl. 2000;21(6):848-54.
Gerena, R. L., Eguchi, N., Urade, Y., & Killian, G. J. (2000). Stage and region-specific localization of lipocalin-type prostaglandin D synthase in the adult murine testis and epididymis. Journal of Andrology, 21(6), 848-54.
Gerena RL, et al. Stage and Region-specific Localization of Lipocalin-type Prostaglandin D Synthase in the Adult Murine Testis and Epididymis. J Androl. 2000 Nov-Dec;21(6):848-54. PubMed PMID: 11105911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stage and region-specific localization of lipocalin-type prostaglandin D synthase in the adult murine testis and epididymis. AU - Gerena,R L, AU - Eguchi,N, AU - Urade,Y, AU - Killian,G J, PY - 2000/12/6/pubmed PY - 2001/6/2/medline PY - 2000/12/6/entrez SP - 848 EP - 54 JF - Journal of andrology JO - J Androl VL - 21 IS - 6 N2 - Lipocalin-type prostaglandin D synthase in semen has been associated with male fertility, although this relationship is not well defined. To gain insight into potential mechanisms, the objective of the present study was to immunocytochemically localize lipocalin-type prostaglandin D synthase within the testis, efferent ducts, and 4 segments of mouse epididymis. In the testis, immunoperoxidase staining was localized within the Sertoli cells only at stages VI-VIII of the spermatogenic cycle, which is just prior to spermiation. Intense staining was also evident throughout the interstitial tissue, including Leydig cells. The entire epithelium of the efferent ducts, including ciliated and nonciliated cells, was immunoreactive. A distinct pattern of immunostaining for lipocalin-type prostaglandin D synthase was observed in different regions of epididymis, suggesting a possible role in sperm maturation. Staining for lipocalin-type prostaglandin D synthase was strikingly absent in the initial segment. In caput epididymidis, staining was evident throughout the cell cytoplasm of principal cells with some cells more intensely stained than adjacent ones. In the corpus region, overall staining intensity decreased and appeared to be concentrated in the apical region of principal cells, but some cells were completely unreactive. Reaction product in the cauda region was heavily concentrated on microvilli and within the epididymal lumen. In all epididymal regions, expression of lipocalin-type prostaglandin D synthase was specific to epithelial principal cells; no immunoreactivity was apparent in other cell types. The specific localization of lipocalin-type prostaglandin D synthase within the testicular interstitial tissue, Sertoli cells, and principal cells of caput epididymidis strongly suggests that this protein plays an integral role in both the development and maturation of sperm. SN - 0196-3635 UR - https://www.unboundmedicine.com/medline/citation/11105911/Stage_and_region_specific_localization_of_lipocalin_type_prostaglandin_D_synthase_in_the_adult_murine_testis_and_epididymis_ DB - PRIME DP - Unbound Medicine ER -