Tags

Type your tag names separated by a space and hit enter

Determination of the designer drugs 3, 4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine with HPLC and fluorescence detection in whole blood, serum, vitreous humor, and urine.
Clin Chem. 2000 Dec; 46(12):1968-77.CC

Abstract

BACKGROUND

The popular designer drugs 3, 4-methylenedioxymethamphetamine (MDMA) and 3, 4-methylenedioxyethylamphetamine (MDEA) can be determined in serum, whole blood, and urine, but also in vitreous humor. The latter matrix is interesting when dealing with decomposed bodies in a toxicological setting.

METHODS

After extraction, chromatographic separation was achieved on a narrow-bore C(18) column by gradient elution with fluorometric detection; results were confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

RESULTS

The method was linear over the range of 2-1000 microg/L for whole blood, serum, and vitreous humor, and 0.1-5 mg/L for urine. Extraction recoveries were >70%, imprecision (CV) was 2.5-19%, and analytical recoveries were 95.5-104.4%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.8 and 2 microg/L, respectively, for whole blood, serum, and vitreous humor, and 2.5 microg/L and 0.1 mg/L, respectively, for urine. Excellent correlations between the quantitative LC-fluorescence and LC-MS/MS results were obtained. We found the following concentrations in a thanatochemical distribution study in rabbits: in serum, 5.3-685 microg/L for MDMA and from the LOQ to 14.5 microg/L for 3, 4-methylenedioxyamphetamine (MDA); in whole blood, 19.7-710 microg/L for MDMA and from the LOQ to 17.8 microg/L for MDA; in vitreous humor, 12.1-97.8 microg/L for MDMA and from the LOQ to 3.86 microg/L for MDA. In routine toxicological urine samples, concentrations ranged from LOQ to 14.62 mg/L for MDA, from LOQ to 157 mg/L for MDMA, and from LOQ to 32.54 mg/L for MDEA.

CONCLUSIONS

The HPLC method described is sensitive, specific, and suitable for the determination of MDMA, MDEA, and MDA in whole blood, serum, vitreous humor, and urine.

Links

Aggregator Full Text

Authors+Show Affiliations

Clauwaert KM
Laboratory of Toxicology and Laboratory of Medicinal Chemistry, Ghent University, Harelbekestraat 72, B-9000 Gent, Belgium.
Van Bocxlaer JF
No affiliation info available
De Letter EA
No affiliation info available
Van Calenbergh S
No affiliation info available
Lambert WE
No affiliation info available
De Leenheer AP
No affiliation info available

MeSH

3,4-MethylenedioxyamphetamineAnimalsChromatography, High Pressure LiquidDesigner DrugsHumansMass SpectrometryN-Methyl-3,4-methylenedioxyamphetamineRabbitsReproducibility of ResultsSensitivity and SpecificitySpectrometry, FluorescenceSubstance Abuse DetectionVitreous Body

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11106329

Citation

Clauwaert, K M., et al. "Determination of the Designer Drugs 3, 4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine With HPLC and Fluorescence Detection in Whole Blood, Serum, Vitreous Humor, and Urine." Clinical Chemistry, vol. 46, no. 12, 2000, pp. 1968-77.
Clauwaert KM, Van Bocxlaer JF, De Letter EA, et al. Determination of the designer drugs 3, 4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine with HPLC and fluorescence detection in whole blood, serum, vitreous humor, and urine. Clin Chem. 2000;46(12):1968-77.
Clauwaert, K. M., Van Bocxlaer, J. F., De Letter, E. A., Van Calenbergh, S., Lambert, W. E., & De Leenheer, A. P. (2000). Determination of the designer drugs 3, 4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine with HPLC and fluorescence detection in whole blood, serum, vitreous humor, and urine. Clinical Chemistry, 46(12), 1968-77.
Clauwaert KM, et al. Determination of the Designer Drugs 3, 4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine With HPLC and Fluorescence Detection in Whole Blood, Serum, Vitreous Humor, and Urine. Clin Chem. 2000;46(12):1968-77. PubMed PMID: 11106329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of the designer drugs 3, 4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine with HPLC and fluorescence detection in whole blood, serum, vitreous humor, and urine. AU - Clauwaert,K M, AU - Van Bocxlaer,J F, AU - De Letter,E A, AU - Van Calenbergh,S, AU - Lambert,W E, AU - De Leenheer,A P, PY - 2000/12/6/pubmed PY - 2001/2/28/medline PY - 2000/12/6/entrez SP - 1968 EP - 77 JF - Clinical chemistry JO - Clin Chem VL - 46 IS - 12 N2 - BACKGROUND: The popular designer drugs 3, 4-methylenedioxymethamphetamine (MDMA) and 3, 4-methylenedioxyethylamphetamine (MDEA) can be determined in serum, whole blood, and urine, but also in vitreous humor. The latter matrix is interesting when dealing with decomposed bodies in a toxicological setting. METHODS: After extraction, chromatographic separation was achieved on a narrow-bore C(18) column by gradient elution with fluorometric detection; results were confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS: The method was linear over the range of 2-1000 microg/L for whole blood, serum, and vitreous humor, and 0.1-5 mg/L for urine. Extraction recoveries were >70%, imprecision (CV) was 2.5-19%, and analytical recoveries were 95.5-104.4%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.8 and 2 microg/L, respectively, for whole blood, serum, and vitreous humor, and 2.5 microg/L and 0.1 mg/L, respectively, for urine. Excellent correlations between the quantitative LC-fluorescence and LC-MS/MS results were obtained. We found the following concentrations in a thanatochemical distribution study in rabbits: in serum, 5.3-685 microg/L for MDMA and from the LOQ to 14.5 microg/L for 3, 4-methylenedioxyamphetamine (MDA); in whole blood, 19.7-710 microg/L for MDMA and from the LOQ to 17.8 microg/L for MDA; in vitreous humor, 12.1-97.8 microg/L for MDMA and from the LOQ to 3.86 microg/L for MDA. In routine toxicological urine samples, concentrations ranged from LOQ to 14.62 mg/L for MDA, from LOQ to 157 mg/L for MDMA, and from LOQ to 32.54 mg/L for MDEA. CONCLUSIONS: The HPLC method described is sensitive, specific, and suitable for the determination of MDMA, MDEA, and MDA in whole blood, serum, vitreous humor, and urine. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/11106329/Determination_of_the_designer_drugs_3_4_methylenedioxymethamphetamine_34_methylenedioxyethylamphetamine_and_34_methylenedioxyamphetamine_with_HPLC_and_fluorescence_detection_in_whole_blood_serum_vitreous_humor_and_urine_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11106329.ui DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓4]

Related Citations

More