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Fibroblast contractility: usual interstitial pneumonia and nonspecific interstitial pneumonia.
Am J Respir Crit Care Med. 2000 Dec; 162(6):2259-64.AJ

Abstract

The aim of this study was to compare the function of lung fibroblasts obtained from surgically biopsied specimens of patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (UIP; n = 5), nonspecific interstitial pneumonia (NSIP; n = 5), and normal parts of surgically resected lungs (control; n = 5). The results showed that (1) fibroblasts obtained from UIP showed increased contractility compared with those obtained from NSIP or controls (UIP, 72.7 +/- 6.21%; NSIP, 32.8 +/- 5.46; controls, 28.5 +/- 3.51, p < 0.01 in UIP versus NSIP or control); (2) this increase in contractility was consistent with enhanced F-actin content in fibroblasts; (3) conditioned media from UIP fibroblast cultures enhanced control fibroblast contractility, whereas those obtained from NSIP or controls did not; (4) the 180 and 25 kD products representing the contractility in conditioned media were identified as fibronectin (ED-A domain) and TGF-beta1 by immunoblots, respectively; (5) the UIP-conditioned media contained higher amounts of fibronectin or TGF-beta 1 (fibronectin: UIP 289 +/- 47.1 ng/ml, NSIP 121 +/- 23.0, control 118 +/- 16.0; TGF-beta1: UIP 798 +/- 119 pg/ml, NSIP 246 +/- 69.1, control 247 +/- 53.6, p < 0.01 in UIP versus NSIP or control); () the contractility positively correlated with the amount of either fibronectin (r = 0.867, p < 0.001, n = 15) or TGF-beta 1 (r = 0.939, p < 0.001, n = 15), respectively. Thus, UIP fibroblasts showed greater contractility than did NSIP fibroblasts and up-regulated control fibroblasts.

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Authors+Show Affiliations

Miki H
Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Japan.
Mio T
No affiliation info available
Nagai S
No affiliation info available
Hoshino Y
No affiliation info available
Nagao T
No affiliation info available
Kitaichi M
No affiliation info available
Izumi T
No affiliation info available

MeSH

ActinsAdenocarcinomaAnalysis of VarianceBiopsyCells, CulturedCulture Media, Serum-FreeFemaleFibroblastsGelsHamartomaHumansLungLung DiseasesLung Diseases, InterstitialLung NeoplasmsMaleMiddle AgedTransforming Growth Factor betaTransforming Growth Factor beta1

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11112149

Citation

Miki, H, et al. "Fibroblast Contractility: Usual Interstitial Pneumonia and Nonspecific Interstitial Pneumonia." American Journal of Respiratory and Critical Care Medicine, vol. 162, no. 6, 2000, pp. 2259-64.
Miki H, Mio T, Nagai S, et al. Fibroblast contractility: usual interstitial pneumonia and nonspecific interstitial pneumonia. Am J Respir Crit Care Med. 2000;162(6):2259-64.
Miki, H., Mio, T., Nagai, S., Hoshino, Y., Nagao, T., Kitaichi, M., & Izumi, T. (2000). Fibroblast contractility: usual interstitial pneumonia and nonspecific interstitial pneumonia. American Journal of Respiratory and Critical Care Medicine, 162(6), 2259-64.
Miki H, et al. Fibroblast Contractility: Usual Interstitial Pneumonia and Nonspecific Interstitial Pneumonia. Am J Respir Crit Care Med. 2000;162(6):2259-64. PubMed PMID: 11112149.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fibroblast contractility: usual interstitial pneumonia and nonspecific interstitial pneumonia. AU - Miki,H, AU - Mio,T, AU - Nagai,S, AU - Hoshino,Y, AU - Nagao,T, AU - Kitaichi,M, AU - Izumi,T, PY - 2000/12/9/pubmed PY - 2001/2/28/medline PY - 2000/12/9/entrez SP - 2259 EP - 64 JF - American journal of respiratory and critical care medicine JO - Am J Respir Crit Care Med VL - 162 IS - 6 N2 - The aim of this study was to compare the function of lung fibroblasts obtained from surgically biopsied specimens of patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (UIP; n = 5), nonspecific interstitial pneumonia (NSIP; n = 5), and normal parts of surgically resected lungs (control; n = 5). The results showed that (1) fibroblasts obtained from UIP showed increased contractility compared with those obtained from NSIP or controls (UIP, 72.7 +/- 6.21%; NSIP, 32.8 +/- 5.46; controls, 28.5 +/- 3.51, p < 0.01 in UIP versus NSIP or control); (2) this increase in contractility was consistent with enhanced F-actin content in fibroblasts; (3) conditioned media from UIP fibroblast cultures enhanced control fibroblast contractility, whereas those obtained from NSIP or controls did not; (4) the 180 and 25 kD products representing the contractility in conditioned media were identified as fibronectin (ED-A domain) and TGF-beta1 by immunoblots, respectively; (5) the UIP-conditioned media contained higher amounts of fibronectin or TGF-beta 1 (fibronectin: UIP 289 +/- 47.1 ng/ml, NSIP 121 +/- 23.0, control 118 +/- 16.0; TGF-beta1: UIP 798 +/- 119 pg/ml, NSIP 246 +/- 69.1, control 247 +/- 53.6, p < 0.01 in UIP versus NSIP or control); () the contractility positively correlated with the amount of either fibronectin (r = 0.867, p < 0.001, n = 15) or TGF-beta 1 (r = 0.939, p < 0.001, n = 15), respectively. Thus, UIP fibroblasts showed greater contractility than did NSIP fibroblasts and up-regulated control fibroblasts. SN - 1073-449X UR - https://www.unboundmedicine.com/medline/citation/11112149/Fibroblast_contractility:_usual_interstitial_pneumonia_and_nonspecific_interstitial_pneumonia_ L2 - https://www.atsjournals.org/doi/10.1164/ajrccm.162.6.9812029?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -