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Fibroblast contractility: usual interstitial pneumonia and nonspecific interstitial pneumonia.
Am J Respir Crit Care Med. 2000 Dec; 162(6):2259-64.AJ

Abstract

The aim of this study was to compare the function of lung fibroblasts obtained from surgically biopsied specimens of patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (UIP; n = 5), nonspecific interstitial pneumonia (NSIP; n = 5), and normal parts of surgically resected lungs (control; n = 5). The results showed that (1) fibroblasts obtained from UIP showed increased contractility compared with those obtained from NSIP or controls (UIP, 72.7 +/- 6.21%; NSIP, 32.8 +/- 5.46; controls, 28.5 +/- 3.51, p < 0.01 in UIP versus NSIP or control); (2) this increase in contractility was consistent with enhanced F-actin content in fibroblasts; (3) conditioned media from UIP fibroblast cultures enhanced control fibroblast contractility, whereas those obtained from NSIP or controls did not; (4) the 180 and 25 kD products representing the contractility in conditioned media were identified as fibronectin (ED-A domain) and TGF-beta1 by immunoblots, respectively; (5) the UIP-conditioned media contained higher amounts of fibronectin or TGF-beta 1 (fibronectin: UIP 289 +/- 47.1 ng/ml, NSIP 121 +/- 23.0, control 118 +/- 16.0; TGF-beta1: UIP 798 +/- 119 pg/ml, NSIP 246 +/- 69.1, control 247 +/- 53.6, p < 0.01 in UIP versus NSIP or control); () the contractility positively correlated with the amount of either fibronectin (r = 0.867, p < 0.001, n = 15) or TGF-beta 1 (r = 0.939, p < 0.001, n = 15), respectively. Thus, UIP fibroblasts showed greater contractility than did NSIP fibroblasts and up-regulated control fibroblasts.

Authors+Show Affiliations

Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11112149

Citation

Miki, H, et al. "Fibroblast Contractility: Usual Interstitial Pneumonia and Nonspecific Interstitial Pneumonia." American Journal of Respiratory and Critical Care Medicine, vol. 162, no. 6, 2000, pp. 2259-64.
Miki H, Mio T, Nagai S, et al. Fibroblast contractility: usual interstitial pneumonia and nonspecific interstitial pneumonia. Am J Respir Crit Care Med. 2000;162(6):2259-64.
Miki, H., Mio, T., Nagai, S., Hoshino, Y., Nagao, T., Kitaichi, M., & Izumi, T. (2000). Fibroblast contractility: usual interstitial pneumonia and nonspecific interstitial pneumonia. American Journal of Respiratory and Critical Care Medicine, 162(6), 2259-64.
Miki H, et al. Fibroblast Contractility: Usual Interstitial Pneumonia and Nonspecific Interstitial Pneumonia. Am J Respir Crit Care Med. 2000;162(6):2259-64. PubMed PMID: 11112149.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fibroblast contractility: usual interstitial pneumonia and nonspecific interstitial pneumonia. AU - Miki,H, AU - Mio,T, AU - Nagai,S, AU - Hoshino,Y, AU - Nagao,T, AU - Kitaichi,M, AU - Izumi,T, PY - 2000/12/9/pubmed PY - 2001/2/28/medline PY - 2000/12/9/entrez SP - 2259 EP - 64 JF - American journal of respiratory and critical care medicine JO - Am J Respir Crit Care Med VL - 162 IS - 6 N2 - The aim of this study was to compare the function of lung fibroblasts obtained from surgically biopsied specimens of patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (UIP; n = 5), nonspecific interstitial pneumonia (NSIP; n = 5), and normal parts of surgically resected lungs (control; n = 5). The results showed that (1) fibroblasts obtained from UIP showed increased contractility compared with those obtained from NSIP or controls (UIP, 72.7 +/- 6.21%; NSIP, 32.8 +/- 5.46; controls, 28.5 +/- 3.51, p < 0.01 in UIP versus NSIP or control); (2) this increase in contractility was consistent with enhanced F-actin content in fibroblasts; (3) conditioned media from UIP fibroblast cultures enhanced control fibroblast contractility, whereas those obtained from NSIP or controls did not; (4) the 180 and 25 kD products representing the contractility in conditioned media were identified as fibronectin (ED-A domain) and TGF-beta1 by immunoblots, respectively; (5) the UIP-conditioned media contained higher amounts of fibronectin or TGF-beta 1 (fibronectin: UIP 289 +/- 47.1 ng/ml, NSIP 121 +/- 23.0, control 118 +/- 16.0; TGF-beta1: UIP 798 +/- 119 pg/ml, NSIP 246 +/- 69.1, control 247 +/- 53.6, p < 0.01 in UIP versus NSIP or control); () the contractility positively correlated with the amount of either fibronectin (r = 0.867, p < 0.001, n = 15) or TGF-beta 1 (r = 0.939, p < 0.001, n = 15), respectively. Thus, UIP fibroblasts showed greater contractility than did NSIP fibroblasts and up-regulated control fibroblasts. SN - 1073-449X UR - https://www.unboundmedicine.com/medline/citation/11112149/Fibroblast_contractility:_usual_interstitial_pneumonia_and_nonspecific_interstitial_pneumonia_ L2 - https://www.atsjournals.org/doi/10.1164/ajrccm.162.6.9812029?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -