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Antibody-dependent induction of type I interferons by poliovirus in human mononuclear blood cells requires the type II fcgamma receptor (CD32).
Virology 2000; 278(1):86-94V

Abstract

The induction of type I interferons (IFNs) in peripheral blood mononuclear cells (PBMCs) can be triggered by viral infection or exposure to viral glycoproteins. Here we show that the IFN-alpha-inducing capacity of attenuated poliovirus vaccine strains is dramatically enhanced in the presence of human polyvalent immunoglobulin G (IgG). The transcription of both IFN-alpha and IFN-beta genes was detected by RT-PCR in stimulated cells. This antibody-dependent activation of type I IFNs genes was also observed with Formalin-inactivated or UV-inactivated poliovirus, but not with empty poliovirus capsids. The ability of poliovirus-antibody complexes to induce IFN-alpha was specifically inhibited when PBMCs were preincubated with an excess of the Fc fragment of IgG. Monoclonal antibodies directed to FcgammaRII (CD32) were also inhibitory, whereas antibodies to the two other classes of Fcgamma receptors, CD16 and CD64, were not. Also, aggregation of FcgammaRII by anti-CD32 antibodies alone failed to induce IFN-alpha production. Our results suggest that induction of type I interferons by poliovirus-antibody complexes depends on CD32-mediated phagocytosis of RNA-containing viral particles. As suggested by the results of an ELISPOT analysis, only a fraction of the IFN-alpha-producing cells are able to synthesize IFN-alpha in response to poliovirus-IgG complexes.

Authors+Show Affiliations

Laboratoire de Virologie, Hôpital Saint-Vincent-de-Paul, Université Paris V, 82 avenue Denfert-Rochereau, Paris Cedex 14, 75674, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11112484

Citation

Palmer, P, et al. "Antibody-dependent Induction of Type I Interferons By Poliovirus in Human Mononuclear Blood Cells Requires the Type II Fcgamma Receptor (CD32)." Virology, vol. 278, no. 1, 2000, pp. 86-94.
Palmer P, Charley B, Rombaut B, et al. Antibody-dependent induction of type I interferons by poliovirus in human mononuclear blood cells requires the type II fcgamma receptor (CD32). Virology. 2000;278(1):86-94.
Palmer, P., Charley, B., Rombaut, B., Daëron, M., & Lebon, P. (2000). Antibody-dependent induction of type I interferons by poliovirus in human mononuclear blood cells requires the type II fcgamma receptor (CD32). Virology, 278(1), pp. 86-94.
Palmer P, et al. Antibody-dependent Induction of Type I Interferons By Poliovirus in Human Mononuclear Blood Cells Requires the Type II Fcgamma Receptor (CD32). Virology. 2000 Dec 5;278(1):86-94. PubMed PMID: 11112484.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibody-dependent induction of type I interferons by poliovirus in human mononuclear blood cells requires the type II fcgamma receptor (CD32). AU - Palmer,P, AU - Charley,B, AU - Rombaut,B, AU - Daëron,M, AU - Lebon,P, PY - 2000/12/9/pubmed PY - 2001/2/28/medline PY - 2000/12/9/entrez SP - 86 EP - 94 JF - Virology JO - Virology VL - 278 IS - 1 N2 - The induction of type I interferons (IFNs) in peripheral blood mononuclear cells (PBMCs) can be triggered by viral infection or exposure to viral glycoproteins. Here we show that the IFN-alpha-inducing capacity of attenuated poliovirus vaccine strains is dramatically enhanced in the presence of human polyvalent immunoglobulin G (IgG). The transcription of both IFN-alpha and IFN-beta genes was detected by RT-PCR in stimulated cells. This antibody-dependent activation of type I IFNs genes was also observed with Formalin-inactivated or UV-inactivated poliovirus, but not with empty poliovirus capsids. The ability of poliovirus-antibody complexes to induce IFN-alpha was specifically inhibited when PBMCs were preincubated with an excess of the Fc fragment of IgG. Monoclonal antibodies directed to FcgammaRII (CD32) were also inhibitory, whereas antibodies to the two other classes of Fcgamma receptors, CD16 and CD64, were not. Also, aggregation of FcgammaRII by anti-CD32 antibodies alone failed to induce IFN-alpha production. Our results suggest that induction of type I interferons by poliovirus-antibody complexes depends on CD32-mediated phagocytosis of RNA-containing viral particles. As suggested by the results of an ELISPOT analysis, only a fraction of the IFN-alpha-producing cells are able to synthesize IFN-alpha in response to poliovirus-IgG complexes. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/11112484/Antibody_dependent_induction_of_type_I_interferons_by_poliovirus_in_human_mononuclear_blood_cells_requires_the_type_II_fcgamma_receptor__CD32__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(00)90627-5 DB - PRIME DP - Unbound Medicine ER -