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A simplified cyclic adenosine monophosphate-mediated sweat rate test for quantitative measure of cystic fibrosis transmembrane regulator (CFTR) function.
J Pediatr. 2000 Dec; 137(6):849-55.JPed

Abstract

OBJECTIVE

Sweat production is stimulated by both cholinergic and beta-adrenergic pathways in the sweat gland secretory coil. beta-Adrenergic pathway-mediated sweating is absent in cystic fibrosis (CF) because cyclic adenosine monophosphate (cAMP)-mediated chloride transport through the cystic fibrosis transmembrane regulator (CFTR) is disrupted. We report the development of a rapid, reproducible, macroscopic, and quantitative methodology to test the hypothesis that beta-adrenergic sweat rate discriminates among 3 different CFTR phenotypes-CF, heterozygote CF carriers, and non-CF.

STUDY DESIGN

Intradermal injection of a mixture of 50 micromol/L isoproterenol, 5 mmol/L aminophylline (to potentiate the beta-adrenergic stimulation), and 140 micromol/L atropine (to block potential cholinergic stimulation) in lactated Ringer's solution was performed in duplicate on one forearm. A single injection of 0.5 mmol/L methacholine to stimulate sweat production by the cholinergic pathway was performed on the other forearm. Sweat rate was determined as the amount of sweat collected on filter paper over 20 minutes.

RESULTS AND CONCLUSIONS

Median cAMP-mediated sweat rates were 1.45 mg/20 min (CF, n = 29), 2.55 mg/20 min (CF heterozygote carriers, n = 30), and 3.65 mg/20 min (non-CF, n = 30) and were significantly different in all 3 groups (P =.0001, Kruskal-Wallis test). Methacholine-stimulated sweat rates were similar for all 3 groups. The cAMP-mediated sweat rate test may be a useful endpoint for studies of new agents to increase the function of CFTR.

Authors+Show Affiliations

Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11113843

Citation

Callen, A, et al. "A Simplified Cyclic Adenosine Monophosphate-mediated Sweat Rate Test for Quantitative Measure of Cystic Fibrosis Transmembrane Regulator (CFTR) Function." The Journal of Pediatrics, vol. 137, no. 6, 2000, pp. 849-55.
Callen A, Diener-West M, Zeitlin PL, et al. A simplified cyclic adenosine monophosphate-mediated sweat rate test for quantitative measure of cystic fibrosis transmembrane regulator (CFTR) function. J Pediatr. 2000;137(6):849-55.
Callen, A., Diener-West, M., Zeitlin, P. L., & Rubenstein, R. C. (2000). A simplified cyclic adenosine monophosphate-mediated sweat rate test for quantitative measure of cystic fibrosis transmembrane regulator (CFTR) function. The Journal of Pediatrics, 137(6), 849-55.
Callen A, et al. A Simplified Cyclic Adenosine Monophosphate-mediated Sweat Rate Test for Quantitative Measure of Cystic Fibrosis Transmembrane Regulator (CFTR) Function. J Pediatr. 2000;137(6):849-55. PubMed PMID: 11113843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simplified cyclic adenosine monophosphate-mediated sweat rate test for quantitative measure of cystic fibrosis transmembrane regulator (CFTR) function. AU - Callen,A, AU - Diener-West,M, AU - Zeitlin,P L, AU - Rubenstein,R C, PY - 2000/12/13/pubmed PY - 2001/2/28/medline PY - 2000/12/13/entrez SP - 849 EP - 55 JF - The Journal of pediatrics JO - J. Pediatr. VL - 137 IS - 6 N2 - OBJECTIVE: Sweat production is stimulated by both cholinergic and beta-adrenergic pathways in the sweat gland secretory coil. beta-Adrenergic pathway-mediated sweating is absent in cystic fibrosis (CF) because cyclic adenosine monophosphate (cAMP)-mediated chloride transport through the cystic fibrosis transmembrane regulator (CFTR) is disrupted. We report the development of a rapid, reproducible, macroscopic, and quantitative methodology to test the hypothesis that beta-adrenergic sweat rate discriminates among 3 different CFTR phenotypes-CF, heterozygote CF carriers, and non-CF. STUDY DESIGN: Intradermal injection of a mixture of 50 micromol/L isoproterenol, 5 mmol/L aminophylline (to potentiate the beta-adrenergic stimulation), and 140 micromol/L atropine (to block potential cholinergic stimulation) in lactated Ringer's solution was performed in duplicate on one forearm. A single injection of 0.5 mmol/L methacholine to stimulate sweat production by the cholinergic pathway was performed on the other forearm. Sweat rate was determined as the amount of sweat collected on filter paper over 20 minutes. RESULTS AND CONCLUSIONS: Median cAMP-mediated sweat rates were 1.45 mg/20 min (CF, n = 29), 2.55 mg/20 min (CF heterozygote carriers, n = 30), and 3.65 mg/20 min (non-CF, n = 30) and were significantly different in all 3 groups (P =.0001, Kruskal-Wallis test). Methacholine-stimulated sweat rates were similar for all 3 groups. The cAMP-mediated sweat rate test may be a useful endpoint for studies of new agents to increase the function of CFTR. SN - 0022-3476 UR - https://www.unboundmedicine.com/medline/citation/11113843/A_simplified_cyclic_adenosine_monophosphate_mediated_sweat_rate_test_for_quantitative_measure_of_cystic_fibrosis_transmembrane_regulator__CFTR__function_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-3476(00)97327-X DB - PRIME DP - Unbound Medicine ER -