Tags

Type your tag names separated by a space and hit enter

Characterization of Aspergillus spores by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 2000; 14(24):2393-400.RC

Abstract

The intact fungal spores of several strains of four Aspergillus species, Aspergillus flavus, A. oryzae, A. parasiticus, and A. sojae, were directly analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. Very simple MALDI mass spectra are obtained by directly mixing spores with a matrix such as alpha-cyano-4-hydroxycinnamic acid or sinapinic acid. The mass spectra are obtained from the ablation of cell walls of spores owing to the acidity of the matrix solution. The MALDI results show that aflatoxigenic strains and non-aflatoxigenic strains have different mass peak profiles. Furthermore, the MALDI results of non-aflatoxigenic A. flavus and A. parasiticus spores resemble those of the closely related A. oryzae and A. sojae spores, respectively.

Authors+Show Affiliations

Li TY
Institute of Toxicology, Tzu Chi University, Hualien 970, Taiwan.
Liu BH
No affiliation info available
Chen YC
No affiliation info available

MeSH

AspergillusCell WallCoumaric AcidsCrops, AgriculturalSpecies SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpores, Fungal

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11114056

Citation

Li, T Y., et al. "Characterization of Aspergillus Spores By Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 14, no. 24, 2000, pp. 2393-400.
Li TY, Liu BH, Chen YC. Characterization of Aspergillus spores by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2000;14(24):2393-400.
Li, T. Y., Liu, B. H., & Chen, Y. C. (2000). Characterization of Aspergillus spores by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 14(24), 2393-400.
Li TY, Liu BH, Chen YC. Characterization of Aspergillus Spores By Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 2000;14(24):2393-400. PubMed PMID: 11114056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Aspergillus spores by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. AU - Li,T Y, AU - Liu,B H, AU - Chen,Y C, PY - 2000/12/13/pubmed PY - 2001/3/3/medline PY - 2000/12/13/entrez SP - 2393 EP - 400 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 14 IS - 24 N2 - The intact fungal spores of several strains of four Aspergillus species, Aspergillus flavus, A. oryzae, A. parasiticus, and A. sojae, were directly analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. Very simple MALDI mass spectra are obtained by directly mixing spores with a matrix such as alpha-cyano-4-hydroxycinnamic acid or sinapinic acid. The mass spectra are obtained from the ablation of cell walls of spores owing to the acidity of the matrix solution. The MALDI results show that aflatoxigenic strains and non-aflatoxigenic strains have different mass peak profiles. Furthermore, the MALDI results of non-aflatoxigenic A. flavus and A. parasiticus spores resemble those of the closely related A. oryzae and A. sojae spores, respectively. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/11114056/Characterization_of_Aspergillus_spores_by_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -