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Determination of gamma-hydroxybutyric acid (GHB) in plasma and urine by headspace solid-phase microextraction and gas chromatography/positive ion chemical ionization mass spectrometry.
Rapid Commun Mass Spectrom. 2000; 14(24):2401-7.RC

Abstract

A new method for the qualitative and quantitative analysis of gamma-hydroxybutyric acid (GHB) in plasma and urine samples is described. It involves the conversion of GHB to gamma-butyrolactone (GBL), its subsequent headspace solid-phase microextraction (SPME), and detection by gas chromatography/positive ion chemical ionization mass spectrometry (GC/PICI-MS), using D(6)-GBL as internal standard. The assay is linear over a plasma GHB range of 1-100 microg/mL (n = 5, r = 0.999) and a urine GHB range of 5-150 microg/mL (n = 5, r = 0. 998). Relative intra- and inter-assay standard deviations, determined for plasma and urine samples at 5 and 50 microg/mL, are all below 5%. The method is simple, specific and reasonably fast. It may be applied for clinical and forensic toxicology as well as for purposes of therapeutic drug monitoring.

Authors+Show Affiliations

Forensic Toxicology and Antidoping, University Hospital of Padova, Via Falloppio 50, I-35121 Padova, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11114057

Citation

Frison, G, et al. "Determination of Gamma-hydroxybutyric Acid (GHB) in Plasma and Urine By Headspace Solid-phase Microextraction and Gas Chromatography/positive Ion Chemical Ionization Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 14, no. 24, 2000, pp. 2401-7.
Frison G, Tedeschi L, Maietti S, et al. Determination of gamma-hydroxybutyric acid (GHB) in plasma and urine by headspace solid-phase microextraction and gas chromatography/positive ion chemical ionization mass spectrometry. Rapid Commun Mass Spectrom. 2000;14(24):2401-7.
Frison, G., Tedeschi, L., Maietti, S., & Ferrara, S. D. (2000). Determination of gamma-hydroxybutyric acid (GHB) in plasma and urine by headspace solid-phase microextraction and gas chromatography/positive ion chemical ionization mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 14(24), 2401-7.
Frison G, et al. Determination of Gamma-hydroxybutyric Acid (GHB) in Plasma and Urine By Headspace Solid-phase Microextraction and Gas Chromatography/positive Ion Chemical Ionization Mass Spectrometry. Rapid Commun Mass Spectrom. 2000;14(24):2401-7. PubMed PMID: 11114057.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of gamma-hydroxybutyric acid (GHB) in plasma and urine by headspace solid-phase microextraction and gas chromatography/positive ion chemical ionization mass spectrometry. AU - Frison,G, AU - Tedeschi,L, AU - Maietti,S, AU - Ferrara,S D, PY - 2000/12/13/pubmed PY - 2001/3/3/medline PY - 2000/12/13/entrez SP - 2401 EP - 7 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 14 IS - 24 N2 - A new method for the qualitative and quantitative analysis of gamma-hydroxybutyric acid (GHB) in plasma and urine samples is described. It involves the conversion of GHB to gamma-butyrolactone (GBL), its subsequent headspace solid-phase microextraction (SPME), and detection by gas chromatography/positive ion chemical ionization mass spectrometry (GC/PICI-MS), using D(6)-GBL as internal standard. The assay is linear over a plasma GHB range of 1-100 microg/mL (n = 5, r = 0.999) and a urine GHB range of 5-150 microg/mL (n = 5, r = 0. 998). Relative intra- and inter-assay standard deviations, determined for plasma and urine samples at 5 and 50 microg/mL, are all below 5%. The method is simple, specific and reasonably fast. It may be applied for clinical and forensic toxicology as well as for purposes of therapeutic drug monitoring. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/11114057/Determination_of_gamma_hydroxybutyric_acid__GHB__in_plasma_and_urine_by_headspace_solid_phase_microextraction_and_gas_chromatography/positive_ion_chemical_ionization_mass_spectrometry_ L2 - https://doi.org/10.1002/1097-0231(20001230)14:24<2401::AID-RCM179>3.0.CO;2-I DB - PRIME DP - Unbound Medicine ER -