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Epstein-Barr virus-encoded poly(A)(-) RNA supports Burkitt's lymphoma growth through interleukin-10 induction.
EMBO J 2000; 19(24):6742-50EJ

Abstract

Akata and Mutu cell lines are derived from Burkitt's lymphoma (BL) and retain the in vivo phenotype of Epstein-Barr virus (EBV) expression that is characterized by expression of EBV-determined nuclear antigen 1 (EBNA1), EBV-encoded RNAs (EBERs) and transcripts from the BAM:HI A region (BARF0). We found that EBV-positive Akata and Mutu cell clones expressed higher levels of interleukin (IL)-10 than their EBV-negative subclones at the transcriptional level. Transfection of an individual EBV latent gene into EBV-negative Akata cells revealed that EBERs were responsible for IL-10 induction. Recombinant IL-10 enabled EBV-negative Akata cells to grow in low (0.1%) serum conditions. On the other hand, growth of EBV-positive Akata cells was blocked by treatment either with an anti-IL-10 antibody or antisense oligonucleotide against IL-10. EBV-positive BL biopsies consistently expressed IL-10, but EBV-negative BL biopsies did not. These results suggest that IL-10 induced by EBERs acts as an autocrine growth factor for BL. EBERs, EBER1 and EBER2, are non-polyadenylated RNAs and are 166 and 172 nucleotides long, respectively. The present findings indicate that RNA molecules could regulate cell growth.

Authors+Show Affiliations

Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University and First Department of Surgery, Hokkaido University School of Medicine, N15 W7, Kita-ku, Sapporo 060-8638, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11118209

Citation

Kitagawa, N, et al. "Epstein-Barr Virus-encoded poly(A)(-) RNA Supports Burkitt's Lymphoma Growth Through Interleukin-10 Induction." The EMBO Journal, vol. 19, no. 24, 2000, pp. 6742-50.
Kitagawa N, Goto M, Kurozumi K, et al. Epstein-Barr virus-encoded poly(A)(-) RNA supports Burkitt's lymphoma growth through interleukin-10 induction. EMBO J. 2000;19(24):6742-50.
Kitagawa, N., Goto, M., Kurozumi, K., Maruo, S., Fukayama, M., Naoe, T., ... Takada, K. (2000). Epstein-Barr virus-encoded poly(A)(-) RNA supports Burkitt's lymphoma growth through interleukin-10 induction. The EMBO Journal, 19(24), pp. 6742-50.
Kitagawa N, et al. Epstein-Barr Virus-encoded poly(A)(-) RNA Supports Burkitt's Lymphoma Growth Through Interleukin-10 Induction. EMBO J. 2000 Dec 15;19(24):6742-50. PubMed PMID: 11118209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus-encoded poly(A)(-) RNA supports Burkitt's lymphoma growth through interleukin-10 induction. AU - Kitagawa,N, AU - Goto,M, AU - Kurozumi,K, AU - Maruo,S, AU - Fukayama,M, AU - Naoe,T, AU - Yasukawa,M, AU - Hino,K, AU - Suzuki,T, AU - Todo,S, AU - Takada,K, PY - 2000/12/16/pubmed PY - 2001/3/3/medline PY - 2000/12/16/entrez SP - 6742 EP - 50 JF - The EMBO journal JO - EMBO J. VL - 19 IS - 24 N2 - Akata and Mutu cell lines are derived from Burkitt's lymphoma (BL) and retain the in vivo phenotype of Epstein-Barr virus (EBV) expression that is characterized by expression of EBV-determined nuclear antigen 1 (EBNA1), EBV-encoded RNAs (EBERs) and transcripts from the BAM:HI A region (BARF0). We found that EBV-positive Akata and Mutu cell clones expressed higher levels of interleukin (IL)-10 than their EBV-negative subclones at the transcriptional level. Transfection of an individual EBV latent gene into EBV-negative Akata cells revealed that EBERs were responsible for IL-10 induction. Recombinant IL-10 enabled EBV-negative Akata cells to grow in low (0.1%) serum conditions. On the other hand, growth of EBV-positive Akata cells was blocked by treatment either with an anti-IL-10 antibody or antisense oligonucleotide against IL-10. EBV-positive BL biopsies consistently expressed IL-10, but EBV-negative BL biopsies did not. These results suggest that IL-10 induced by EBERs acts as an autocrine growth factor for BL. EBERs, EBER1 and EBER2, are non-polyadenylated RNAs and are 166 and 172 nucleotides long, respectively. The present findings indicate that RNA molecules could regulate cell growth. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/11118209/Epstein_Barr_virus_encoded_poly_A_____RNA_supports_Burkitt's_lymphoma_growth_through_interleukin_10_induction_ L2 - https://doi.org/10.1093/emboj/19.24.6742 DB - PRIME DP - Unbound Medicine ER -