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A reevaluation of the peroxynitrite scavenging activity of some dietary phenolics.
Biochem Biophys Res Commun. 2000 Dec 20; 279(2):692-9.BB

Abstract

Peroxynitrite is implicated in many diseases. Hence, there is considerable interest in potential therapeutic peroxynitrite scavengers. Diet-derived phenolics have been claimed to be powerful peroxynitrite scavengers. However, the reactivity of peroxynitrite can be significantly modified by bicarbonate and this has not been considered in evaluations of the scavenging activity of phenols. Bicarbonate (25 mM) significantly decreased the ability of several phenolic compounds (caffeic acid, o- and p-coumaric acid, gallic acid, ferulic acid) but not others (catechin and epicatechin) to inhibit peroxynitrite-mediated tyrosine nitration. Bicarbonate (25 mM) also decreased the ability of catechin, epicatechin, quercetin and ferulic acid but not chlorogenic acid, gallic acid, caffeic acid and o-coumaric acid to inhibit peroxynitrite-mediated alpha(1)-antiproteinase inactivation. These results show that physiological concentrations of bicarbonate substantially modify the ability of dietary phenolics to prevent peroxynitrite-mediated reactions. When assessing compounds for peroxynitrite scavenging, experiments should be conducted in the presence of bicarbonate to avoid misleading results.

Authors+Show Affiliations

International Antioxidant Research Centre, GKT School of Biomedical Sciences, Third Floor Hodgkin Building, St. Thomas Street, London, SE1 9RT, United Kingdom.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11118347

Citation

Ketsawatsakul, U, et al. "A Reevaluation of the Peroxynitrite Scavenging Activity of some Dietary Phenolics." Biochemical and Biophysical Research Communications, vol. 279, no. 2, 2000, pp. 692-9.
Ketsawatsakul U, Whiteman M, Halliwell B. A reevaluation of the peroxynitrite scavenging activity of some dietary phenolics. Biochem Biophys Res Commun. 2000;279(2):692-9.
Ketsawatsakul, U., Whiteman, M., & Halliwell, B. (2000). A reevaluation of the peroxynitrite scavenging activity of some dietary phenolics. Biochemical and Biophysical Research Communications, 279(2), 692-9.
Ketsawatsakul U, Whiteman M, Halliwell B. A Reevaluation of the Peroxynitrite Scavenging Activity of some Dietary Phenolics. Biochem Biophys Res Commun. 2000 Dec 20;279(2):692-9. PubMed PMID: 11118347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A reevaluation of the peroxynitrite scavenging activity of some dietary phenolics. AU - Ketsawatsakul,U, AU - Whiteman,M, AU - Halliwell,B, PY - 2000/12/19/pubmed PY - 2001/3/3/medline PY - 2000/12/19/entrez SP - 692 EP - 9 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 279 IS - 2 N2 - Peroxynitrite is implicated in many diseases. Hence, there is considerable interest in potential therapeutic peroxynitrite scavengers. Diet-derived phenolics have been claimed to be powerful peroxynitrite scavengers. However, the reactivity of peroxynitrite can be significantly modified by bicarbonate and this has not been considered in evaluations of the scavenging activity of phenols. Bicarbonate (25 mM) significantly decreased the ability of several phenolic compounds (caffeic acid, o- and p-coumaric acid, gallic acid, ferulic acid) but not others (catechin and epicatechin) to inhibit peroxynitrite-mediated tyrosine nitration. Bicarbonate (25 mM) also decreased the ability of catechin, epicatechin, quercetin and ferulic acid but not chlorogenic acid, gallic acid, caffeic acid and o-coumaric acid to inhibit peroxynitrite-mediated alpha(1)-antiproteinase inactivation. These results show that physiological concentrations of bicarbonate substantially modify the ability of dietary phenolics to prevent peroxynitrite-mediated reactions. When assessing compounds for peroxynitrite scavenging, experiments should be conducted in the presence of bicarbonate to avoid misleading results. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/11118347/A_reevaluation_of_the_peroxynitrite_scavenging_activity_of_some_dietary_phenolics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(00)94014-2 DB - PRIME DP - Unbound Medicine ER -