TY - JOUR T1 - Cloning and expression of cDNAs encoding two enzymes of the MEP pathway in Catharanthus roseus. AU - Veau,B, AU - Courtois,M, AU - Oudin,A, AU - Chénieux,J C, AU - Rideau,M, AU - Clastre,M, PY - 2000/12/19/pubmed PY - 2001/3/3/medline PY - 2000/12/19/entrez SP - 159 EP - 63 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1517 IS - 1 N2 - Two periwinkle cDNAs (crdxr and crmecs) encoding enzymes of the non-mevalonate terpenoid pathway were characterized using reverse transcription-PCR strategy based on the design of degenerated oligonucleotides. The deduced amino acid sequence of crdxr is homologue to 1-deoxy-D-xylulose 5-phosphate reductoisomerases. Crmecs represents the first plant cDNA encoding a protein similar to the 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Escherichia coli. Expression of crdxr and crmecs genes was up-regulated in periwinkle cells producing monoterpenoid indole alkaloids. Involvement of the 2C-methyl-D-erythritol 4-phosphate pathway in alkaloid biosynthesis is discussed. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/11118631/Cloning_and_expression_of_cDNAs_encoding_two_enzymes_of_the_MEP_pathway_in_Catharanthus_roseus_ DB - PRIME DP - Unbound Medicine ER -