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Highly sensitive analysis of methamphetamine and amphetamine in human whole blood using headspace solid-phase microextraction and gas chromatography-mass spectrometry.
Forensic Sci Int. 2001 Feb 01; 116(1):15-22.FS

Abstract

A simple and highly sensitive method for analysis of derivatized methamphetamine (MA) and amphetamine (AM) in whole blood was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry electron impact ionization selected ion monitoring (GC-MS-EI-SIM). A whole blood sample, deuterated-MA (d(5)-MA), as an internal standard (IS), tri-n-propylamine and pentafluorobenzyl bromide were placed in a vial. The vial was heated and stirred at 90 degrees C for 30min. Then the extraction fiber of the SPME was exposed at 90 degrees C for 30min in the headspace of the vial while being stirred. The derivatives adsorbed on the fiber were desorbed by exposing the fiber in the injection port of a GC-MS. The calibration curves showed linearity in the range of 0.5-1000ng/g for both MA and AM. The time for analysis was about 80min per sample. In addition, this proposed method was applied to two autopsy cases where MA ingestion was suspected. In one case, MA and AM concentrations in the mixed left and right heart blood were 165 and 36.9ng/g, respectively. In the other case, MA and AM concentrations were 1.79 and 0.119 microg/g in the left heart blood, and 1.27 and 0.074 microg/g in the right heart blood, respectively.

Authors+Show Affiliations

Department of Legal Medicine, Faculty of Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11118748

Citation

Okajima, K, et al. "Highly Sensitive Analysis of Methamphetamine and Amphetamine in Human Whole Blood Using Headspace Solid-phase Microextraction and Gas Chromatography-mass Spectrometry." Forensic Science International, vol. 116, no. 1, 2001, pp. 15-22.
Okajima K, Namera A, Yashiki M, et al. Highly sensitive analysis of methamphetamine and amphetamine in human whole blood using headspace solid-phase microextraction and gas chromatography-mass spectrometry. Forensic Sci Int. 2001;116(1):15-22.
Okajima, K., Namera, A., Yashiki, M., Tsukue, I., & Kojima, T. (2001). Highly sensitive analysis of methamphetamine and amphetamine in human whole blood using headspace solid-phase microextraction and gas chromatography-mass spectrometry. Forensic Science International, 116(1), 15-22.
Okajima K, et al. Highly Sensitive Analysis of Methamphetamine and Amphetamine in Human Whole Blood Using Headspace Solid-phase Microextraction and Gas Chromatography-mass Spectrometry. Forensic Sci Int. 2001 Feb 1;116(1):15-22. PubMed PMID: 11118748.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive analysis of methamphetamine and amphetamine in human whole blood using headspace solid-phase microextraction and gas chromatography-mass spectrometry. AU - Okajima,K, AU - Namera,A, AU - Yashiki,M, AU - Tsukue,I, AU - Kojima,T, PY - 2000/12/19/pubmed PY - 2001/3/3/medline PY - 2000/12/19/entrez SP - 15 EP - 22 JF - Forensic science international JO - Forensic Sci. Int. VL - 116 IS - 1 N2 - A simple and highly sensitive method for analysis of derivatized methamphetamine (MA) and amphetamine (AM) in whole blood was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry electron impact ionization selected ion monitoring (GC-MS-EI-SIM). A whole blood sample, deuterated-MA (d(5)-MA), as an internal standard (IS), tri-n-propylamine and pentafluorobenzyl bromide were placed in a vial. The vial was heated and stirred at 90 degrees C for 30min. Then the extraction fiber of the SPME was exposed at 90 degrees C for 30min in the headspace of the vial while being stirred. The derivatives adsorbed on the fiber were desorbed by exposing the fiber in the injection port of a GC-MS. The calibration curves showed linearity in the range of 0.5-1000ng/g for both MA and AM. The time for analysis was about 80min per sample. In addition, this proposed method was applied to two autopsy cases where MA ingestion was suspected. In one case, MA and AM concentrations in the mixed left and right heart blood were 165 and 36.9ng/g, respectively. In the other case, MA and AM concentrations were 1.79 and 0.119 microg/g in the left heart blood, and 1.27 and 0.074 microg/g in the right heart blood, respectively. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/11118748/Highly_sensitive_analysis_of_methamphetamine_and_amphetamine_in_human_whole_blood_using_headspace_solid_phase_microextraction_and_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(00)00341-8 DB - PRIME DP - Unbound Medicine ER -