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Epitopes targeted by bullous pemphigoid T lymphocytes and autoantibodies map to the same sites on the bullous pemphigoid 180 ectodomain.
J Invest Dermatol. 2000 Dec; 115(6):955-61.JI

Abstract

Bullous pemphigoid is a blistering skin disease characterized by autoantibodies directed against the NC16A domain of bullous pemphigoid 180 (collagen XVII), a transmembrane protein of epidermal basal cells. Passive transfer studies in mice have shown that antibodies that bind to this immunodominant region of bullous pemphigoid 180 are capable of inducing a skin disease that closely mimics bullous pemphigoid, supporting the hypothesis that epitopes within NC16A are involved in the pathogenesis of bullous pemphigoid. In this study, we examined the autoimmune T cell response in bullous pemphigoid patients. T cells from eight of 12 bullous pemphigoid patients, all of whom had circulating anti-bullous pemphigoid 180 autoantibodies, showed a specific proliferative response to recombinant forms of NC16A. T cell lines and clones developed from four of these patients recognize the same NC16A peptides as those targeted by autoantibodies from the corresponding individuals. These NC16A-responding T lymphocytes express alpha/beta T cell receptors and CD4 memory T cell surface markers and exhibited a Th1/Th2 mixed cytokine profile that may support the production of antibodies. This new information will aid in defining the key steps involved in the development of the autoimmune response in bullous pemphigoid.

Authors+Show Affiliations

Department of Dermatology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27759, USA. mslin@med.unc.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11121125

Citation

Lin, M S., et al. "Epitopes Targeted By Bullous Pemphigoid T Lymphocytes and Autoantibodies Map to the Same Sites On the Bullous Pemphigoid 180 Ectodomain." The Journal of Investigative Dermatology, vol. 115, no. 6, 2000, pp. 955-61.
Lin MS, Fu CL, Giudice GJ, et al. Epitopes targeted by bullous pemphigoid T lymphocytes and autoantibodies map to the same sites on the bullous pemphigoid 180 ectodomain. J Invest Dermatol. 2000;115(6):955-61.
Lin, M. S., Fu, C. L., Giudice, G. J., Olague-Marchan, M., Lazaro, A. M., Stastny, P., & Diaz, L. A. (2000). Epitopes targeted by bullous pemphigoid T lymphocytes and autoantibodies map to the same sites on the bullous pemphigoid 180 ectodomain. The Journal of Investigative Dermatology, 115(6), 955-61.
Lin MS, et al. Epitopes Targeted By Bullous Pemphigoid T Lymphocytes and Autoantibodies Map to the Same Sites On the Bullous Pemphigoid 180 Ectodomain. J Invest Dermatol. 2000;115(6):955-61. PubMed PMID: 11121125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epitopes targeted by bullous pemphigoid T lymphocytes and autoantibodies map to the same sites on the bullous pemphigoid 180 ectodomain. AU - Lin,M S, AU - Fu,C L, AU - Giudice,G J, AU - Olague-Marchan,M, AU - Lazaro,A M, AU - Stastny,P, AU - Diaz,L A, PY - 2000/12/20/pubmed PY - 2001/3/3/medline PY - 2000/12/20/entrez SP - 955 EP - 61 JF - The Journal of investigative dermatology JO - J Invest Dermatol VL - 115 IS - 6 N2 - Bullous pemphigoid is a blistering skin disease characterized by autoantibodies directed against the NC16A domain of bullous pemphigoid 180 (collagen XVII), a transmembrane protein of epidermal basal cells. Passive transfer studies in mice have shown that antibodies that bind to this immunodominant region of bullous pemphigoid 180 are capable of inducing a skin disease that closely mimics bullous pemphigoid, supporting the hypothesis that epitopes within NC16A are involved in the pathogenesis of bullous pemphigoid. In this study, we examined the autoimmune T cell response in bullous pemphigoid patients. T cells from eight of 12 bullous pemphigoid patients, all of whom had circulating anti-bullous pemphigoid 180 autoantibodies, showed a specific proliferative response to recombinant forms of NC16A. T cell lines and clones developed from four of these patients recognize the same NC16A peptides as those targeted by autoantibodies from the corresponding individuals. These NC16A-responding T lymphocytes express alpha/beta T cell receptors and CD4 memory T cell surface markers and exhibited a Th1/Th2 mixed cytokine profile that may support the production of antibodies. This new information will aid in defining the key steps involved in the development of the autoimmune response in bullous pemphigoid. SN - 0022-202X UR - https://www.unboundmedicine.com/medline/citation/11121125/Epitopes_targeted_by_bullous_pemphigoid_T_lymphocytes_and_autoantibodies_map_to_the_same_sites_on_the_bullous_pemphigoid_180_ectodomain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-202X(15)41066-8 DB - PRIME DP - Unbound Medicine ER -