Tags

Type your tag names separated by a space and hit enter

Biliary lipid composition in patients with cholesterol and pigment gallstones and gallstone-free subjects: deoxycholic acid does not contribute to formation of cholesterol gallstones.
Eur J Clin Invest 2000; 30(12):1099-106EJ

Abstract

BACKGROUND

Four main disturbances have been attributed to cholesterol gallstone disease: hypersecretion of cholesterol from the liver with cholesterol supersaturation in bile; disturbed motility with defective absorption and secretion by the gallbladder; increased crystallisation of cholesterol in the gallbladder bile; and slow intestinal transit with increased amount of deoxycholic acid in the bile acid pool. We aimed to evaluate the biliary lipid composition in a large series of gallstone patients, with emphasis on the amount of deoxycholic acid and with respect to number of stones, compared to gallstone free subjects.

MATERIALS AND METHODS

Bile was sampled during operations through puncture of the gallbladder from 145 cholesterol gallstone patients, 23 patients with pigment stones and 87 gallstone free patients undergoing cholecystectomy. Biliary lipid composition, cholesterol saturation, bile acid composition, nucleation time and cholesterol crystals were analysed.

RESULTS

The patients with cholesterol gallstones showed higher molar percentage of cholesterol, lower total biliary lipid concentration, higher cholesterol saturation, shorter nucleation time and higher proportion of crystals in bile than the other groups. The nucleation time was significantly shorter in multiple cholesterol gallstone patients, but this was not due to higher cholesterol saturation. Male cholesterol gallstone patients showed higher cholesterol levels, lower total biliary lipid concentration, and higher cholesterol saturation in bile than female patients. There was no difference in biliary content of deoxycholic acid, but significantly lower content of cholic acid in gallstone patients compared to gallstone free patients.

CONCLUSIONS

We conclude that deoxycholic acid does not contribute to gallstone formation in cholesterol gallstone patients. The short nucleation time in patients with multiple cholesterol stones is not due to higher cholesterol saturation.

Authors+Show Affiliations

Karolinska Institutet, Stockholm, Sweden. Ulf.Gustafsson@kir.ds.sll.seNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11122325

Citation

Gustafsson, U, et al. "Biliary Lipid Composition in Patients With Cholesterol and Pigment Gallstones and Gallstone-free Subjects: Deoxycholic Acid Does Not Contribute to Formation of Cholesterol Gallstones." European Journal of Clinical Investigation, vol. 30, no. 12, 2000, pp. 1099-106.
Gustafsson U, Sahlin S, Einarsson C. Biliary lipid composition in patients with cholesterol and pigment gallstones and gallstone-free subjects: deoxycholic acid does not contribute to formation of cholesterol gallstones. Eur J Clin Invest. 2000;30(12):1099-106.
Gustafsson, U., Sahlin, S., & Einarsson, C. (2000). Biliary lipid composition in patients with cholesterol and pigment gallstones and gallstone-free subjects: deoxycholic acid does not contribute to formation of cholesterol gallstones. European Journal of Clinical Investigation, 30(12), pp. 1099-106.
Gustafsson U, Sahlin S, Einarsson C. Biliary Lipid Composition in Patients With Cholesterol and Pigment Gallstones and Gallstone-free Subjects: Deoxycholic Acid Does Not Contribute to Formation of Cholesterol Gallstones. Eur J Clin Invest. 2000;30(12):1099-106. PubMed PMID: 11122325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biliary lipid composition in patients with cholesterol and pigment gallstones and gallstone-free subjects: deoxycholic acid does not contribute to formation of cholesterol gallstones. AU - Gustafsson,U, AU - Sahlin,S, AU - Einarsson,C, PY - 2000/12/21/pubmed PY - 2001/3/17/medline PY - 2000/12/21/entrez SP - 1099 EP - 106 JF - European journal of clinical investigation JO - Eur. J. Clin. Invest. VL - 30 IS - 12 N2 - BACKGROUND: Four main disturbances have been attributed to cholesterol gallstone disease: hypersecretion of cholesterol from the liver with cholesterol supersaturation in bile; disturbed motility with defective absorption and secretion by the gallbladder; increased crystallisation of cholesterol in the gallbladder bile; and slow intestinal transit with increased amount of deoxycholic acid in the bile acid pool. We aimed to evaluate the biliary lipid composition in a large series of gallstone patients, with emphasis on the amount of deoxycholic acid and with respect to number of stones, compared to gallstone free subjects. MATERIALS AND METHODS: Bile was sampled during operations through puncture of the gallbladder from 145 cholesterol gallstone patients, 23 patients with pigment stones and 87 gallstone free patients undergoing cholecystectomy. Biliary lipid composition, cholesterol saturation, bile acid composition, nucleation time and cholesterol crystals were analysed. RESULTS: The patients with cholesterol gallstones showed higher molar percentage of cholesterol, lower total biliary lipid concentration, higher cholesterol saturation, shorter nucleation time and higher proportion of crystals in bile than the other groups. The nucleation time was significantly shorter in multiple cholesterol gallstone patients, but this was not due to higher cholesterol saturation. Male cholesterol gallstone patients showed higher cholesterol levels, lower total biliary lipid concentration, and higher cholesterol saturation in bile than female patients. There was no difference in biliary content of deoxycholic acid, but significantly lower content of cholic acid in gallstone patients compared to gallstone free patients. CONCLUSIONS: We conclude that deoxycholic acid does not contribute to gallstone formation in cholesterol gallstone patients. The short nucleation time in patients with multiple cholesterol stones is not due to higher cholesterol saturation. SN - 0014-2972 UR - https://www.unboundmedicine.com/medline/citation/11122325/Biliary_lipid_composition_in_patients_with_cholesterol_and_pigment_gallstones_and_gallstone_free_subjects:_deoxycholic_acid_does_not_contribute_to_formation_of_cholesterol_gallstones_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-2972&date=2000&volume=30&issue=12&spage=1099 DB - PRIME DP - Unbound Medicine ER -