Tags

Type your tag names separated by a space and hit enter

IL-12 and IL-18 are increased and stimulate IFN-gamma production in sarcoid lungs.
J Immunol. 2001 Jan 01; 166(1):642-9.JI

Abstract

Sarcoidosis is a systemic chronic granulomatous disease of unknown cause. Recent investigations revealed that the cytokine profile in inflamed lesions of sarcoidosis is Th1 dominant. To obtain better immunopathologic understanding of sarcoidosis, we examined the expression of IL-12 and IL-18 and their roles in IFN-gamma production in pulmonary sarcoidosis. Sarcoid cases had significantly elevated levels of IL-12 (p40 and p70) and IL-18 in bronchoalveolar lavage (BAL) fluids compared with healthy subjects. IL-12 p70 and IL-18 were immunohistochemically expressed in the epithelioid cells and giant cells of sarcoid granulomas. Significant induction of IFN-gamma, IL-12 p70, and IL-18 was observed from sarcoid BAL fluid cells with LPS stimulation, whereas LPS tended to induce only IL-12 p70 in BAL fluid cells from healthy subjects. Sarcoid cases had significantly greater IFN-gamma induction with LPS stimulation than healthy subjects did. IL-18 mRNA expression was observed in freshly isolated sarcoid BAL fluid cells as well as in LPS-stimulated sarcoid BAL fluid cells, but IFN-gamma and IL-12 mRNA expression was observed only in LPS-stimulated BAL fluid cells. Treatment with anti-IL-12- and anti-IL-18-neutralizing Abs significantly inhibited IFN-gamma production from LPS-stimulated BAL fluid cells of sarcoid cases. Coadministration of rIL-12 or rIL-18 induced greater IFN-gamma production in sarcoid BAL fluid cells than in normal BAL fluid cells. We concluded that bioactive IL-12 and IL-18 were produced in sarcoid BAL fluid cells and synergistically induced IFN-gamma production, indicating important cytokines in the Th1 response of sarcoidosis.

Authors+Show Affiliations

Hokkaido Branch of Japan Anti-Tuberculosis Association, Sapporo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11123348

Citation

Shigehara, K, et al. "IL-12 and IL-18 Are Increased and Stimulate IFN-gamma Production in Sarcoid Lungs." Journal of Immunology (Baltimore, Md. : 1950), vol. 166, no. 1, 2001, pp. 642-9.
Shigehara K, Shijubo N, Ohmichi M, et al. IL-12 and IL-18 are increased and stimulate IFN-gamma production in sarcoid lungs. J Immunol. 2001;166(1):642-9.
Shigehara, K., Shijubo, N., Ohmichi, M., Takahashi, R., Kon, S., Okamura, H., Kurimoto, M., Hiraga, Y., Tatsuno, T., Abe, S., & Sato, N. (2001). IL-12 and IL-18 are increased and stimulate IFN-gamma production in sarcoid lungs. Journal of Immunology (Baltimore, Md. : 1950), 166(1), 642-9.
Shigehara K, et al. IL-12 and IL-18 Are Increased and Stimulate IFN-gamma Production in Sarcoid Lungs. J Immunol. 2001 Jan 1;166(1):642-9. PubMed PMID: 11123348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-12 and IL-18 are increased and stimulate IFN-gamma production in sarcoid lungs. AU - Shigehara,K, AU - Shijubo,N, AU - Ohmichi,M, AU - Takahashi,R, AU - Kon,S, AU - Okamura,H, AU - Kurimoto,M, AU - Hiraga,Y, AU - Tatsuno,T, AU - Abe,S, AU - Sato,N, PY - 2000/12/21/pubmed PY - 2001/3/3/medline PY - 2000/12/21/entrez SP - 642 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 166 IS - 1 N2 - Sarcoidosis is a systemic chronic granulomatous disease of unknown cause. Recent investigations revealed that the cytokine profile in inflamed lesions of sarcoidosis is Th1 dominant. To obtain better immunopathologic understanding of sarcoidosis, we examined the expression of IL-12 and IL-18 and their roles in IFN-gamma production in pulmonary sarcoidosis. Sarcoid cases had significantly elevated levels of IL-12 (p40 and p70) and IL-18 in bronchoalveolar lavage (BAL) fluids compared with healthy subjects. IL-12 p70 and IL-18 were immunohistochemically expressed in the epithelioid cells and giant cells of sarcoid granulomas. Significant induction of IFN-gamma, IL-12 p70, and IL-18 was observed from sarcoid BAL fluid cells with LPS stimulation, whereas LPS tended to induce only IL-12 p70 in BAL fluid cells from healthy subjects. Sarcoid cases had significantly greater IFN-gamma induction with LPS stimulation than healthy subjects did. IL-18 mRNA expression was observed in freshly isolated sarcoid BAL fluid cells as well as in LPS-stimulated sarcoid BAL fluid cells, but IFN-gamma and IL-12 mRNA expression was observed only in LPS-stimulated BAL fluid cells. Treatment with anti-IL-12- and anti-IL-18-neutralizing Abs significantly inhibited IFN-gamma production from LPS-stimulated BAL fluid cells of sarcoid cases. Coadministration of rIL-12 or rIL-18 induced greater IFN-gamma production in sarcoid BAL fluid cells than in normal BAL fluid cells. We concluded that bioactive IL-12 and IL-18 were produced in sarcoid BAL fluid cells and synergistically induced IFN-gamma production, indicating important cytokines in the Th1 response of sarcoidosis. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/11123348/IL_12_and_IL_18_are_increased_and_stimulate_IFN_gamma_production_in_sarcoid_lungs_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=11123348 DB - PRIME DP - Unbound Medicine ER -