Tags

Type your tag names separated by a space and hit enter

Protective effects of ifenprodil against glutamate-induced neurotoxicity in cultured retinal neurons.
Graefes Arch Clin Exp Ophthalmol. 2000 Oct; 238(10):846-52.GA

Abstract

PURPOSE

To examine the effects of ifenprodil on glutamate-induced neurotoxicity in cultured retinal neurons.

METHODS

Primary cultures obtained from the fetal rat retina (gestation day 17-19) were used for the experiment. Neurotoxicity effects on retinal cultures were quantitatively assessed by the trypan blue exclusion method. The cells were exposed briefly (10 min) to excitatory amino acids (EAA, 1 mM) and then were incubated for 1 h in an EAA-free medium. Ifenprodil (10 mM) was added for the 10-min exposure to EAA and the subsequent 60-min incubation in an EAA-free medium.

RESULTS

Ifenprodil dose-dependently prevented cell death induced by glutamate or NMDA, but did not affect that induced by kainate. The protective effects of ifenprodil against glutamate neurotoxicity were significantly reduced by spermidine, a polyamine modulatory site agonist, but not by glycine, a strychnine-insensitive glycine site agonist.

CONCLUSION

The findings suggest that ifenprodil protected the cultured retinal cells we used in this study against glutamate neurotoxicity by its inhibitory action on the polyamine modulatory site of the NMDA receptor.

Authors+Show Affiliations

Zhang S
Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Kyoto University, Japan. skashii@kuhp.kyoto-u.ac.jp
Kashii S
No affiliation info available
Yasuyoshi H
No affiliation info available
Kikuchi M
No affiliation info available
Honda Y
No affiliation info available
Kaneda K
No affiliation info available
Sato S
No affiliation info available
Akaike A
No affiliation info available

MeSH

AnimalsCell DeathCell SurvivalCells, CulturedDose-Response Relationship, DrugExcitatory Amino Acid AntagonistsFetusGlutamic AcidNeuronsPiperidinesRatsReceptors, N-Methyl-D-AspartateRetinaSpermidine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11127572

Citation

Zhang, S, et al. "Protective Effects of Ifenprodil Against Glutamate-induced Neurotoxicity in Cultured Retinal Neurons." Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, vol. 238, no. 10, 2000, pp. 846-52.
Zhang S, Kashii S, Yasuyoshi H, et al. Protective effects of ifenprodil against glutamate-induced neurotoxicity in cultured retinal neurons. Graefes Arch Clin Exp Ophthalmol. 2000;238(10):846-52.
Zhang, S., Kashii, S., Yasuyoshi, H., Kikuchi, M., Honda, Y., Kaneda, K., Sato, S., & Akaike, A. (2000). Protective effects of ifenprodil against glutamate-induced neurotoxicity in cultured retinal neurons. Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 238(10), 846-52.
Zhang S, et al. Protective Effects of Ifenprodil Against Glutamate-induced Neurotoxicity in Cultured Retinal Neurons. Graefes Arch Clin Exp Ophthalmol. 2000;238(10):846-52. PubMed PMID: 11127572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of ifenprodil against glutamate-induced neurotoxicity in cultured retinal neurons. AU - Zhang,S, AU - Kashii,S, AU - Yasuyoshi,H, AU - Kikuchi,M, AU - Honda,Y, AU - Kaneda,K, AU - Sato,S, AU - Akaike,A, PY - 2000/12/29/pubmed PY - 2001/2/28/medline PY - 2000/12/29/entrez SP - 846 EP - 52 JF - Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie JO - Graefes Arch Clin Exp Ophthalmol VL - 238 IS - 10 N2 - PURPOSE: To examine the effects of ifenprodil on glutamate-induced neurotoxicity in cultured retinal neurons. METHODS: Primary cultures obtained from the fetal rat retina (gestation day 17-19) were used for the experiment. Neurotoxicity effects on retinal cultures were quantitatively assessed by the trypan blue exclusion method. The cells were exposed briefly (10 min) to excitatory amino acids (EAA, 1 mM) and then were incubated for 1 h in an EAA-free medium. Ifenprodil (10 mM) was added for the 10-min exposure to EAA and the subsequent 60-min incubation in an EAA-free medium. RESULTS: Ifenprodil dose-dependently prevented cell death induced by glutamate or NMDA, but did not affect that induced by kainate. The protective effects of ifenprodil against glutamate neurotoxicity were significantly reduced by spermidine, a polyamine modulatory site agonist, but not by glycine, a strychnine-insensitive glycine site agonist. CONCLUSION: The findings suggest that ifenprodil protected the cultured retinal cells we used in this study against glutamate neurotoxicity by its inhibitory action on the polyamine modulatory site of the NMDA receptor. SN - 0721-832X UR - https://www.unboundmedicine.com/medline/citation/11127572/Protective_effects_of_ifenprodil_against_glutamate_induced_neurotoxicity_in_cultured_retinal_neurons_ DB - PRIME DP - Unbound Medicine ER -