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Diagnostic value of polymerase chain reaction in blood and aqueous humor in immunocompetent patients with ocular toxoplasmosis.
Retina 2000; 20(6):614-9R

Abstract

PURPOSE

To evaluate the diagnostic value of polymerase chain reaction (PCR) in blood and aqueous humor samples from immunocompetent patients with reactivated ocular toxoplasmosis.

METHODS

Group 1 was composed of seven patients with a clinical diagnosis of reactivated ocular toxoplasmosis. Group 2 consisted of 33 controls. In each subject, blood and aqueous humor samples were obtained for detection of Toxoplasma gondii DNA by means of simple PCR, seminested PCR, and Southern blot hybridization.

RESULTS

Group 1: Simple PCR was positive in 3 of 7 blood samples (42%) and in 2 of 7 (28%) aqueous humor samples. Seminested PCR was positive in 4 of 7 (57%) blood samples and in 3 of 7 (42%) aqueous humor samples. Group 2: Simple and seminested PCR were positive in both samples in 2 of 33 (6%) and 4 of 33 (12%), respectively. Sensitivity 57% (18.41-90.10), specificity 87% (71.80-96.60); positive and negative likelihood ratio 4.38 and 0.49, respectively.

CONCLUSIONS

Polymerase chain reaction can be useful for confirming the diagnosis of ocular toxoplasmosis, especially in those eyes where fundus examination does not yield conclusive results. The detection of T. gondii DNA in blood suggests that reactivation of ocular toxoplasmosis cannot be considered a local event.

Authors+Show Affiliations

Department of Ophthalmology, Hospital Universitario Ramón y Cajal, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

11131414

Citation

Figueroa, M S., et al. "Diagnostic Value of Polymerase Chain Reaction in Blood and Aqueous Humor in Immunocompetent Patients With Ocular Toxoplasmosis." Retina (Philadelphia, Pa.), vol. 20, no. 6, 2000, pp. 614-9.
Figueroa MS, Bou G, Marti-Belda P, et al. Diagnostic value of polymerase chain reaction in blood and aqueous humor in immunocompetent patients with ocular toxoplasmosis. Retina (Philadelphia, Pa). 2000;20(6):614-9.
Figueroa, M. S., Bou, G., Marti-Belda, P., Lopez-Velez, R., & Guerrero, A. (2000). Diagnostic value of polymerase chain reaction in blood and aqueous humor in immunocompetent patients with ocular toxoplasmosis. Retina (Philadelphia, Pa.), 20(6), pp. 614-9.
Figueroa MS, et al. Diagnostic Value of Polymerase Chain Reaction in Blood and Aqueous Humor in Immunocompetent Patients With Ocular Toxoplasmosis. Retina (Philadelphia, Pa). 2000;20(6):614-9. PubMed PMID: 11131414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic value of polymerase chain reaction in blood and aqueous humor in immunocompetent patients with ocular toxoplasmosis. AU - Figueroa,M S, AU - Bou,G, AU - Marti-Belda,P, AU - Lopez-Velez,R, AU - Guerrero,A, PY - 2000/12/29/pubmed PY - 2001/2/28/medline PY - 2000/12/29/entrez SP - 614 EP - 9 JF - Retina (Philadelphia, Pa.) JO - Retina (Philadelphia, Pa.) VL - 20 IS - 6 N2 - PURPOSE: To evaluate the diagnostic value of polymerase chain reaction (PCR) in blood and aqueous humor samples from immunocompetent patients with reactivated ocular toxoplasmosis. METHODS: Group 1 was composed of seven patients with a clinical diagnosis of reactivated ocular toxoplasmosis. Group 2 consisted of 33 controls. In each subject, blood and aqueous humor samples were obtained for detection of Toxoplasma gondii DNA by means of simple PCR, seminested PCR, and Southern blot hybridization. RESULTS: Group 1: Simple PCR was positive in 3 of 7 blood samples (42%) and in 2 of 7 (28%) aqueous humor samples. Seminested PCR was positive in 4 of 7 (57%) blood samples and in 3 of 7 (42%) aqueous humor samples. Group 2: Simple and seminested PCR were positive in both samples in 2 of 33 (6%) and 4 of 33 (12%), respectively. Sensitivity 57% (18.41-90.10), specificity 87% (71.80-96.60); positive and negative likelihood ratio 4.38 and 0.49, respectively. CONCLUSIONS: Polymerase chain reaction can be useful for confirming the diagnosis of ocular toxoplasmosis, especially in those eyes where fundus examination does not yield conclusive results. The detection of T. gondii DNA in blood suggests that reactivation of ocular toxoplasmosis cannot be considered a local event. SN - 0275-004X UR - https://www.unboundmedicine.com/medline/citation/11131414/Diagnostic_value_of_polymerase_chain_reaction_in_blood_and_aqueous_humor_in_immunocompetent_patients_with_ocular_toxoplasmosis_ L2 - http://dx.doi.org/10.1097/00006982-200011000-00005 DB - PRIME DP - Unbound Medicine ER -