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Chloromethane utilization gene cluster from Hyphomicrobium chloromethanicum strain CM2(T) and development of functional gene probes to detect halomethane-degrading bacteria.
Appl Environ Microbiol 2001; 67(1):307-16AE

Abstract

Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA and 35-kDa CmuB) are expressed. Previously, four genes, cmuA, cmuB, cmuC, and purU, were shown to be essential for growth of Methylobacterium chloromethanicum on chloromethane. The cmuA and cmuB genes were used as probes to identify homologs in H. chloromethanicum. A cmu gene cluster (9.5 kb) in H. chloromethanicum contained 10 open reading frames: folD (partial), pduX, orf153, orf207, orf225, cmuB, cmuC, cmuA, fmdB, and paaE (partial). CmuA from H. chloromethanicum (67 kDa) showed high identity to CmuA from M. chloromethanicum and contains an N-terminal methyltransferase domain and a C-terminal corrinoid-binding domain. CmuB from H. chloromethanicum is related to a family of methyl transfer proteins and to the CmuB methyltransferase from M. chloromethanicum. CmuC from H. chloromethanicum shows identity to CmuC from M. chloromethanicum and is a putative methyltransferase. folD codes for a methylene-tetrahydrofolate cyclohydrolase, which may be involved in the C(1) transfer pathway for carbon assimilation and CO(2) production, and paaE codes for a putative redox active protein. Molecular analyses and some preliminary biochemical data indicated that the chloromethane utilization pathway in H. chloromethanicum is similar to the corrinoid-dependent methyl transfer system in M. chloromethanicum. PCR primers were developed for successful amplification of cmuA genes from newly isolated chloromethane utilizers and enrichment cultures.

Authors+Show Affiliations

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11133460

Citation

McAnulla, C, et al. "Chloromethane Utilization Gene Cluster From Hyphomicrobium Chloromethanicum Strain CM2(T) and Development of Functional Gene Probes to Detect Halomethane-degrading Bacteria." Applied and Environmental Microbiology, vol. 67, no. 1, 2001, pp. 307-16.
McAnulla C, Woodall CA, McDonald IR, et al. Chloromethane utilization gene cluster from Hyphomicrobium chloromethanicum strain CM2(T) and development of functional gene probes to detect halomethane-degrading bacteria. Appl Environ Microbiol. 2001;67(1):307-16.
McAnulla, C., Woodall, C. A., McDonald, I. R., Studer, A., Vuilleumier, S., Leisinger, T., & Murrell, J. C. (2001). Chloromethane utilization gene cluster from Hyphomicrobium chloromethanicum strain CM2(T) and development of functional gene probes to detect halomethane-degrading bacteria. Applied and Environmental Microbiology, 67(1), pp. 307-16.
McAnulla C, et al. Chloromethane Utilization Gene Cluster From Hyphomicrobium Chloromethanicum Strain CM2(T) and Development of Functional Gene Probes to Detect Halomethane-degrading Bacteria. Appl Environ Microbiol. 2001;67(1):307-16. PubMed PMID: 11133460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chloromethane utilization gene cluster from Hyphomicrobium chloromethanicum strain CM2(T) and development of functional gene probes to detect halomethane-degrading bacteria. AU - McAnulla,C, AU - Woodall,C A, AU - McDonald,I R, AU - Studer,A, AU - Vuilleumier,S, AU - Leisinger,T, AU - Murrell,J C, PY - 2001/1/3/pubmed PY - 2001/5/18/medline PY - 2001/1/3/entrez SP - 307 EP - 16 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 67 IS - 1 N2 - Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA and 35-kDa CmuB) are expressed. Previously, four genes, cmuA, cmuB, cmuC, and purU, were shown to be essential for growth of Methylobacterium chloromethanicum on chloromethane. The cmuA and cmuB genes were used as probes to identify homologs in H. chloromethanicum. A cmu gene cluster (9.5 kb) in H. chloromethanicum contained 10 open reading frames: folD (partial), pduX, orf153, orf207, orf225, cmuB, cmuC, cmuA, fmdB, and paaE (partial). CmuA from H. chloromethanicum (67 kDa) showed high identity to CmuA from M. chloromethanicum and contains an N-terminal methyltransferase domain and a C-terminal corrinoid-binding domain. CmuB from H. chloromethanicum is related to a family of methyl transfer proteins and to the CmuB methyltransferase from M. chloromethanicum. CmuC from H. chloromethanicum shows identity to CmuC from M. chloromethanicum and is a putative methyltransferase. folD codes for a methylene-tetrahydrofolate cyclohydrolase, which may be involved in the C(1) transfer pathway for carbon assimilation and CO(2) production, and paaE codes for a putative redox active protein. Molecular analyses and some preliminary biochemical data indicated that the chloromethane utilization pathway in H. chloromethanicum is similar to the corrinoid-dependent methyl transfer system in M. chloromethanicum. PCR primers were developed for successful amplification of cmuA genes from newly isolated chloromethane utilizers and enrichment cultures. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/11133460/Chloromethane_utilization_gene_cluster_from_Hyphomicrobium_chloromethanicum_strain_CM2_T__and_development_of_functional_gene_probes_to_detect_halomethane_degrading_bacteria_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=11133460 DB - PRIME DP - Unbound Medicine ER -