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Ionization mechanism of oligonucleotides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 2001; 15(1):57-64.RC

Abstract

The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the bases of A, T, G and C in the matrices 2,5-dihydroxybenzoic acid (2,5-DHB), alpha-cyano-4-hydroxycinnamic acid (alpha-CHCA) and 3-hydroxypicolinic acid (3-HPA) provide an insight into the ionization mechanism of oligonucleotides in MALDI. It was found that the low ion signal from DNA in poly-G in MALDI as reported in earlier work could be attributed to the fact that the base of G is difficult to ionize. Our results suggest that the ionization of DNA in MALDI is dominated by the protonation and deprotonation of bases and it is basically independent of the backbone of DNA. Both the protonation and deprotonation are strongly structure dependent. The protonation is dominated by pre-protonation before laser ablation, while the deprotonation is controlled by the thermal reaction.

Authors+Show Affiliations

Kong Y
Department of Physics, Georgia Southern University, Statesboro, GA 30460, USA.
Zhu Y
No affiliation info available
Zhang JY
No affiliation info available

MeSH

Coumaric AcidsDNAGentisatesHydroxybenzoatesIonsOligonucleotidesPicolinic AcidsProtonsSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11135425

Citation

Kong, Y, et al. "Ionization Mechanism of Oligonucleotides in Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 15, no. 1, 2001, pp. 57-64.
Kong Y, Zhu Y, Zhang JY. Ionization mechanism of oligonucleotides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2001;15(1):57-64.
Kong, Y., Zhu, Y., & Zhang, J. Y. (2001). Ionization mechanism of oligonucleotides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 15(1), 57-64.
Kong Y, Zhu Y, Zhang JY. Ionization Mechanism of Oligonucleotides in Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 2001;15(1):57-64. PubMed PMID: 11135425.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ionization mechanism of oligonucleotides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. AU - Kong,Y, AU - Zhu,Y, AU - Zhang,J Y, PY - 2001/1/3/pubmed PY - 2001/3/10/medline PY - 2001/1/3/entrez SP - 57 EP - 64 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 15 IS - 1 N2 - The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the bases of A, T, G and C in the matrices 2,5-dihydroxybenzoic acid (2,5-DHB), alpha-cyano-4-hydroxycinnamic acid (alpha-CHCA) and 3-hydroxypicolinic acid (3-HPA) provide an insight into the ionization mechanism of oligonucleotides in MALDI. It was found that the low ion signal from DNA in poly-G in MALDI as reported in earlier work could be attributed to the fact that the base of G is difficult to ionize. Our results suggest that the ionization of DNA in MALDI is dominated by the protonation and deprotonation of bases and it is basically independent of the backbone of DNA. Both the protonation and deprotonation are strongly structure dependent. The protonation is dominated by pre-protonation before laser ablation, while the deprotonation is controlled by the thermal reaction. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/11135425/Ionization_mechanism_of_oligonucleotides_in_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -