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Different receptors mediate motor neuron death induced by short and long exposures to excitotoxicity.
Brain Res Bull. 2000 Nov 01; 53(4):383-8.BR

Abstract

We compared the effect of short and long exposures of cultured motor neurons to glutamate and kainate (KA) and studied the receptors involved in these two types of excitotoxicity. There was no difference in the receptor type used between short and long glutamate exposures as activation of the N-methyl-D-asparate (NMDA) receptor was in both cases responsible for the motor neuron death. Cell death through activation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors only became apparent when desensitization of these receptors was prevented. In such conditions, motor neurons became much more sensitive to excitotoxicity, and activation of different types of AMPA receptors mediated motor neuron death after short, compared to long, exposures to the non-desensitizing AMPA receptor agonist, KA. Short KA exposures selectively affected motor neurons containing Ca(2+)-permeable AMPA receptors, as the KA effect was completely inhibited by Joro spider toxin and only motor neurons that were positive for the histochemical Co(2+) staining were killed. A long exposure to KA affected motor neurons through both Ca(2+)-permeable and Ca(2+)-impermeable AMPA receptors. The selective death of motor neurons vs. dorsal horn neurons was observed after short KA exposures indicating that the selective vulnerability of motor neurons to excitotoxicity is related to the presence of Ca(2+)-permeable AMPA receptors.

Authors+Show Affiliations

Laboratory of Neurobiology, Department of Neurology, University of Leuven, Leuven, Belgium. Ludo.Vandenbosch@med.kuleuven.ac.beNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11136993

Citation

Van Den Bosch, L, and W Robberecht. "Different Receptors Mediate Motor Neuron Death Induced By Short and Long Exposures to Excitotoxicity." Brain Research Bulletin, vol. 53, no. 4, 2000, pp. 383-8.
Van Den Bosch L, Robberecht W. Different receptors mediate motor neuron death induced by short and long exposures to excitotoxicity. Brain Res Bull. 2000;53(4):383-8.
Van Den Bosch, L., & Robberecht, W. (2000). Different receptors mediate motor neuron death induced by short and long exposures to excitotoxicity. Brain Research Bulletin, 53(4), 383-8.
Van Den Bosch L, Robberecht W. Different Receptors Mediate Motor Neuron Death Induced By Short and Long Exposures to Excitotoxicity. Brain Res Bull. 2000 Nov 1;53(4):383-8. PubMed PMID: 11136993.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different receptors mediate motor neuron death induced by short and long exposures to excitotoxicity. AU - Van Den Bosch,L, AU - Robberecht,W, PY - 2001/1/4/pubmed PY - 2001/3/3/medline PY - 2001/1/4/entrez SP - 383 EP - 8 JF - Brain research bulletin JO - Brain Res Bull VL - 53 IS - 4 N2 - We compared the effect of short and long exposures of cultured motor neurons to glutamate and kainate (KA) and studied the receptors involved in these two types of excitotoxicity. There was no difference in the receptor type used between short and long glutamate exposures as activation of the N-methyl-D-asparate (NMDA) receptor was in both cases responsible for the motor neuron death. Cell death through activation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors only became apparent when desensitization of these receptors was prevented. In such conditions, motor neurons became much more sensitive to excitotoxicity, and activation of different types of AMPA receptors mediated motor neuron death after short, compared to long, exposures to the non-desensitizing AMPA receptor agonist, KA. Short KA exposures selectively affected motor neurons containing Ca(2+)-permeable AMPA receptors, as the KA effect was completely inhibited by Joro spider toxin and only motor neurons that were positive for the histochemical Co(2+) staining were killed. A long exposure to KA affected motor neurons through both Ca(2+)-permeable and Ca(2+)-impermeable AMPA receptors. The selective death of motor neurons vs. dorsal horn neurons was observed after short KA exposures indicating that the selective vulnerability of motor neurons to excitotoxicity is related to the presence of Ca(2+)-permeable AMPA receptors. SN - 0361-9230 UR - https://www.unboundmedicine.com/medline/citation/11136993/Different_receptors_mediate_motor_neuron_death_induced_by_short_and_long_exposures_to_excitotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0361-9230(00)00371-3 DB - PRIME DP - Unbound Medicine ER -