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Rho-kinase/ROCK is involved in cytokinesis through the phosphorylation of myosin light chain and not ezrin/radixin/moesin proteins at the cleavage furrow.
Oncogene 2000; 19(52):6059-64O

Abstract

The small GTPase Rho and one of its targets, Rho-kinase (also termed ROK or ROCK), are implicated in various cellular functions including stress fiber formation, smooth muscle contraction, tumor cell invasion and cell motility. We have previously reported that Rho-kinase accumulates at the cleavage furrow during cytokinesis in several cultured cells. Here, using Rho-kinase inhibitors, Y-27632 and HA1077, we found that Rho-kinase is responsible for the phosphorylation of myosin regulatory light chain at Ser19 in the cleavage furrow during cytokinesis. On the other hand, phosphorylation of ezrin/radixin/moesin (ERM) proteins at the cleavage furrow was enhanced by the addition of the above Rho-kinase inhibitors. Treatment with Y-27632 strongly enhanced the accumulation of Rho-kinase but not RhoA and citron kinase at the cleavage furrow. Furthermore, the furrow ingression in cytokinesis was significantly prolonged in the presence of Y-27632. These results suggest that Rho-kinase is involved in the progression of cytokinesis through the phosphorylation of several proteins including myosin light chain at the cleavage furrow.

Authors+Show Affiliations

Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11146558

Citation

Kosako, H, et al. "Rho-kinase/ROCK Is Involved in Cytokinesis Through the Phosphorylation of Myosin Light Chain and Not Ezrin/radixin/moesin Proteins at the Cleavage Furrow." Oncogene, vol. 19, no. 52, 2000, pp. 6059-64.
Kosako H, Yoshida T, Matsumura F, et al. Rho-kinase/ROCK is involved in cytokinesis through the phosphorylation of myosin light chain and not ezrin/radixin/moesin proteins at the cleavage furrow. Oncogene. 2000;19(52):6059-64.
Kosako, H., Yoshida, T., Matsumura, F., Ishizaki, T., Narumiya, S., & Inagaki, M. (2000). Rho-kinase/ROCK is involved in cytokinesis through the phosphorylation of myosin light chain and not ezrin/radixin/moesin proteins at the cleavage furrow. Oncogene, 19(52), pp. 6059-64.
Kosako H, et al. Rho-kinase/ROCK Is Involved in Cytokinesis Through the Phosphorylation of Myosin Light Chain and Not Ezrin/radixin/moesin Proteins at the Cleavage Furrow. Oncogene. 2000 Dec 7;19(52):6059-64. PubMed PMID: 11146558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rho-kinase/ROCK is involved in cytokinesis through the phosphorylation of myosin light chain and not ezrin/radixin/moesin proteins at the cleavage furrow. AU - Kosako,H, AU - Yoshida,T, AU - Matsumura,F, AU - Ishizaki,T, AU - Narumiya,S, AU - Inagaki,M, PY - 2001/1/9/pubmed PY - 2001/2/28/medline PY - 2001/1/9/entrez SP - 6059 EP - 64 JF - Oncogene JO - Oncogene VL - 19 IS - 52 N2 - The small GTPase Rho and one of its targets, Rho-kinase (also termed ROK or ROCK), are implicated in various cellular functions including stress fiber formation, smooth muscle contraction, tumor cell invasion and cell motility. We have previously reported that Rho-kinase accumulates at the cleavage furrow during cytokinesis in several cultured cells. Here, using Rho-kinase inhibitors, Y-27632 and HA1077, we found that Rho-kinase is responsible for the phosphorylation of myosin regulatory light chain at Ser19 in the cleavage furrow during cytokinesis. On the other hand, phosphorylation of ezrin/radixin/moesin (ERM) proteins at the cleavage furrow was enhanced by the addition of the above Rho-kinase inhibitors. Treatment with Y-27632 strongly enhanced the accumulation of Rho-kinase but not RhoA and citron kinase at the cleavage furrow. Furthermore, the furrow ingression in cytokinesis was significantly prolonged in the presence of Y-27632. These results suggest that Rho-kinase is involved in the progression of cytokinesis through the phosphorylation of several proteins including myosin light chain at the cleavage furrow. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/11146558/Rho_kinase/ROCK_is_involved_in_cytokinesis_through_the_phosphorylation_of_myosin_light_chain_and_not_ezrin/radixin/moesin_proteins_at_the_cleavage_furrow_ L2 - http://dx.doi.org/10.1038/sj.onc.1203987 DB - PRIME DP - Unbound Medicine ER -