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Temporal expression of growth factor genes during myogenesis of satellite cells derived from the biceps femoris and pectoralis major muscles of the chicken.
J Cell Physiol. 2001 Jan; 186(1):146-52.JC

Abstract

The expression of mRNAs for transforming growth factors (TGF-beta2, myostatin, activin-B, and follistatin), insulin-like growth factors (IGF-I and -II), and fibroblast growth factor (basic, bFGF) was investigated in satellite cells derived from chicken pectoralis major (PM) and biceps femoris (BF) muscles in the stages from initiation of proliferation to fusion. These growth factor gene cDNAs were synthesized by reverse transcriptase polymerase chain reaction (RT-PCR). No myostatin, activin-B, follistatin or bFGF expression was detected in either cell culture at 0 h. TGF-beta2 mRNA level increased at 48 h (P < 0.01) and remained constant through 144 h in both PM and BF satellite cell cultures. The ontogeny of myostatin gene expression with the exception of a sharp increase in BF culture at 72 h (P < 0.01), was nearly identical in both cell cultures. Activin-B mRNA level in PM satellite cells was higher than that in BF satellite cells at 72 h and 120 h (P < 0.01). Follistatin mRNA in PM satellite cells was higher than that in BF satellite cells at 24, 96, and 120 h culture (P < 0.01). No IGF-I gene expression was detected in cell cultures at any time point. IGF-II gene expression in BF satellite cells declined at 96 h (P < 0.01) and remained reduced until 144 h. bFGF mRNA in both satellite cell cultures increased at 24 h (P < 0.05) and remained at this level in BF satellite cells through 144 h.

Authors+Show Affiliations

Division of Animal and Veterinary Sciences, West Virginia University, Morgantown 26506-6108, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11147809

Citation

Kocamis, H, et al. "Temporal Expression of Growth Factor Genes During Myogenesis of Satellite Cells Derived From the Biceps Femoris and Pectoralis Major Muscles of the Chicken." Journal of Cellular Physiology, vol. 186, no. 1, 2001, pp. 146-52.
Kocamis H, McFarland DC, Killefer J. Temporal expression of growth factor genes during myogenesis of satellite cells derived from the biceps femoris and pectoralis major muscles of the chicken. J Cell Physiol. 2001;186(1):146-52.
Kocamis, H., McFarland, D. C., & Killefer, J. (2001). Temporal expression of growth factor genes during myogenesis of satellite cells derived from the biceps femoris and pectoralis major muscles of the chicken. Journal of Cellular Physiology, 186(1), 146-52.
Kocamis H, McFarland DC, Killefer J. Temporal Expression of Growth Factor Genes During Myogenesis of Satellite Cells Derived From the Biceps Femoris and Pectoralis Major Muscles of the Chicken. J Cell Physiol. 2001;186(1):146-52. PubMed PMID: 11147809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal expression of growth factor genes during myogenesis of satellite cells derived from the biceps femoris and pectoralis major muscles of the chicken. AU - Kocamis,H, AU - McFarland,D C, AU - Killefer,J, PY - 2001/1/9/pubmed PY - 2001/2/28/medline PY - 2001/1/9/entrez SP - 146 EP - 52 JF - Journal of cellular physiology JO - J Cell Physiol VL - 186 IS - 1 N2 - The expression of mRNAs for transforming growth factors (TGF-beta2, myostatin, activin-B, and follistatin), insulin-like growth factors (IGF-I and -II), and fibroblast growth factor (basic, bFGF) was investigated in satellite cells derived from chicken pectoralis major (PM) and biceps femoris (BF) muscles in the stages from initiation of proliferation to fusion. These growth factor gene cDNAs were synthesized by reverse transcriptase polymerase chain reaction (RT-PCR). No myostatin, activin-B, follistatin or bFGF expression was detected in either cell culture at 0 h. TGF-beta2 mRNA level increased at 48 h (P < 0.01) and remained constant through 144 h in both PM and BF satellite cell cultures. The ontogeny of myostatin gene expression with the exception of a sharp increase in BF culture at 72 h (P < 0.01), was nearly identical in both cell cultures. Activin-B mRNA level in PM satellite cells was higher than that in BF satellite cells at 72 h and 120 h (P < 0.01). Follistatin mRNA in PM satellite cells was higher than that in BF satellite cells at 24, 96, and 120 h culture (P < 0.01). No IGF-I gene expression was detected in cell cultures at any time point. IGF-II gene expression in BF satellite cells declined at 96 h (P < 0.01) and remained reduced until 144 h. bFGF mRNA in both satellite cell cultures increased at 24 h (P < 0.05) and remained at this level in BF satellite cells through 144 h. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/11147809/Temporal_expression_of_growth_factor_genes_during_myogenesis_of_satellite_cells_derived_from_the_biceps_femoris_and_pectoralis_major_muscles_of_the_chicken_ L2 - https://doi.org/10.1002/1097-4652(200101)186:1&lt;146::AID-JCP1014&gt;3.0.CO;2-Q DB - PRIME DP - Unbound Medicine ER -