Tags

Type your tag names separated by a space and hit enter

Anandamide excites central terminals of dorsal root ganglion neurons via vanilloid receptor-1 activation.
J Neurosci. 2001 Feb 15; 21(4):1104-9.JN

Abstract

Recently, the cannabinoid (CB) receptor agonist anandamide (AEA) has been shown to excite perivascular terminals of primary sensory neurons via activation of the vanilloid receptor-1 (VR-1). To determine whether AEA stimulates central terminals of these neurons, via VR-1 activation, we studied the release of calcitonin gene-related peptide (CGRP)- and substance P (SP)-like immunoreactivities (LI) from slices of rat dorsal spinal cord. Mobilization of Ca(2+) in rat dorsal root ganglion (DRG) neurons in culture was also studied. AEA (0.1-10 micrometer) increased the outflow of CGRP-LI and SP-LI from slices of the rat dorsal spinal cord in a Ca(2+)-dependent manner and increased [Ca(2+)](i) in capsaicin-sensitive cultured DRG neurons. Both effects of AEA were abolished by capsaicin pretreatment and by the VR-1 antagonist capsazepine but not affected by the CB receptor antagonists AM281 or AM630. Both neuropeptide release and Ca(2+) mobilization induced by electrical field stimulation (EFS) were inhibited by a low concentration of AEA (10 nm). Inhibition by AEA of EFS-induced responses was reversed by AM281 and AM630, but was not affected by capsazepine. Results indicate that stimulation of VR-1 with high concentrations of AEA excites central terminals of capsaicin-sensitive DRG neurons, thus causing neuropeptide release in the dorsal spinal cord. This novel activity opposes the CB receptor-mediated inhibitory action of low concentrations AEA. However, only if large amounts of endogenous AEA could be produced at the level of the dorsal spinal cord, they may not inhibit, but rather activate, nociceptive sensory neurons.

Authors+Show Affiliations

Headache Center, Departments of Experimental and Clinical Medicine and Neuroscience, University of Ferrara and Sant'Anna Hospital, 44100 Ferrara, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11160380

Citation

Tognetto, M, et al. "Anandamide Excites Central Terminals of Dorsal Root Ganglion Neurons Via Vanilloid Receptor-1 Activation." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 21, no. 4, 2001, pp. 1104-9.
Tognetto M, Amadesi S, Harrison S, et al. Anandamide excites central terminals of dorsal root ganglion neurons via vanilloid receptor-1 activation. J Neurosci. 2001;21(4):1104-9.
Tognetto, M., Amadesi, S., Harrison, S., Creminon, C., Trevisani, M., Carreras, M., Matera, M., Geppetti, P., & Bianchi, A. (2001). Anandamide excites central terminals of dorsal root ganglion neurons via vanilloid receptor-1 activation. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 21(4), 1104-9.
Tognetto M, et al. Anandamide Excites Central Terminals of Dorsal Root Ganglion Neurons Via Vanilloid Receptor-1 Activation. J Neurosci. 2001 Feb 15;21(4):1104-9. PubMed PMID: 11160380.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anandamide excites central terminals of dorsal root ganglion neurons via vanilloid receptor-1 activation. AU - Tognetto,M, AU - Amadesi,S, AU - Harrison,S, AU - Creminon,C, AU - Trevisani,M, AU - Carreras,M, AU - Matera,M, AU - Geppetti,P, AU - Bianchi,A, PY - 2001/2/13/pubmed PY - 2001/6/8/medline PY - 2001/2/13/entrez SP - 1104 EP - 9 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J Neurosci VL - 21 IS - 4 N2 - Recently, the cannabinoid (CB) receptor agonist anandamide (AEA) has been shown to excite perivascular terminals of primary sensory neurons via activation of the vanilloid receptor-1 (VR-1). To determine whether AEA stimulates central terminals of these neurons, via VR-1 activation, we studied the release of calcitonin gene-related peptide (CGRP)- and substance P (SP)-like immunoreactivities (LI) from slices of rat dorsal spinal cord. Mobilization of Ca(2+) in rat dorsal root ganglion (DRG) neurons in culture was also studied. AEA (0.1-10 micrometer) increased the outflow of CGRP-LI and SP-LI from slices of the rat dorsal spinal cord in a Ca(2+)-dependent manner and increased [Ca(2+)](i) in capsaicin-sensitive cultured DRG neurons. Both effects of AEA were abolished by capsaicin pretreatment and by the VR-1 antagonist capsazepine but not affected by the CB receptor antagonists AM281 or AM630. Both neuropeptide release and Ca(2+) mobilization induced by electrical field stimulation (EFS) were inhibited by a low concentration of AEA (10 nm). Inhibition by AEA of EFS-induced responses was reversed by AM281 and AM630, but was not affected by capsazepine. Results indicate that stimulation of VR-1 with high concentrations of AEA excites central terminals of capsaicin-sensitive DRG neurons, thus causing neuropeptide release in the dorsal spinal cord. This novel activity opposes the CB receptor-mediated inhibitory action of low concentrations AEA. However, only if large amounts of endogenous AEA could be produced at the level of the dorsal spinal cord, they may not inhibit, but rather activate, nociceptive sensory neurons. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/11160380/Anandamide_excites_central_terminals_of_dorsal_root_ganglion_neurons_via_vanilloid_receptor_1_activation_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=11160380 DB - PRIME DP - Unbound Medicine ER -