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Antigen presentation to Th1 but not Th2 cells by macrophages results in nitric oxide production and inhibition of T cell proliferation: interferon-gamma is essential but insufficient.
Cell Immunol. 2000 Dec 15; 206(2):125-35.CI

Abstract

The induction and role of nitric oxide (NO) during antigen presentation by macrophages to T helper (Th) cell subsets was examined. When cultured with Th1 clones, macrophage APC produced NO only in the presence of cognate Ag, which in turn suppressed T cell proliferation. IFN-gamma production by the activated Th1 cells was essential for the induction of NO. Th2 cells presented with the same cognate Ag did not induce NO production and proliferated uninhibited. Coactivation of Th1 and Th2 cells specific for the same Ag indicated that Th2 cells did not inhibit NO production, but were sensitive to NO induced by stimulated Th1 cells. Antigenic activation of Th2 cells in the presence of rIFN-gamma resulted in NO-mediated inhibition of proliferation. Th2 cells provided only a cell-associated cofactor, whereas Th1 cells secreted a soluble cofactor for IFN-gamma as well, i.e., TNF-alpha. Finally, a role for IFN-gamma and NO during immune responses was studied in spleen cells obtained from immunized IFN-gamma(-/-) mice. NO production and subsequent inhibition of Ag-specific proliferation ex vivo was observed only after the addition of rIFN-gamma. These studies suggest an IFN-gamma-dependent regulatory role for NO during Ag-specific Th cell activation involving macrophages, with obvious implications for Th subset-dependent immune responses in general.

Authors+Show Affiliations

Department of Neurology, University of Southern California School of Medicine, Los Angeles, California 90033, USA. vanderve@hsc.usc.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11161444

Citation

van der Veen, R C., et al. "Antigen Presentation to Th1 but Not Th2 Cells By Macrophages Results in Nitric Oxide Production and Inhibition of T Cell Proliferation: Interferon-gamma Is Essential but Insufficient." Cellular Immunology, vol. 206, no. 2, 2000, pp. 125-35.
van der Veen RC, Dietlin TA, Pen L, et al. Antigen presentation to Th1 but not Th2 cells by macrophages results in nitric oxide production and inhibition of T cell proliferation: interferon-gamma is essential but insufficient. Cell Immunol. 2000;206(2):125-35.
van der Veen, R. C., Dietlin, T. A., Pen, L., Gray, J. D., & Hofman, F. M. (2000). Antigen presentation to Th1 but not Th2 cells by macrophages results in nitric oxide production and inhibition of T cell proliferation: interferon-gamma is essential but insufficient. Cellular Immunology, 206(2), 125-35.
van der Veen RC, et al. Antigen Presentation to Th1 but Not Th2 Cells By Macrophages Results in Nitric Oxide Production and Inhibition of T Cell Proliferation: Interferon-gamma Is Essential but Insufficient. Cell Immunol. 2000 Dec 15;206(2):125-35. PubMed PMID: 11161444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigen presentation to Th1 but not Th2 cells by macrophages results in nitric oxide production and inhibition of T cell proliferation: interferon-gamma is essential but insufficient. AU - van der Veen,R C, AU - Dietlin,T A, AU - Pen,L, AU - Gray,J D, AU - Hofman,F M, PY - 2001/2/13/pubmed PY - 2001/3/7/medline PY - 2001/2/13/entrez SP - 125 EP - 35 JF - Cellular immunology JO - Cell. Immunol. VL - 206 IS - 2 N2 - The induction and role of nitric oxide (NO) during antigen presentation by macrophages to T helper (Th) cell subsets was examined. When cultured with Th1 clones, macrophage APC produced NO only in the presence of cognate Ag, which in turn suppressed T cell proliferation. IFN-gamma production by the activated Th1 cells was essential for the induction of NO. Th2 cells presented with the same cognate Ag did not induce NO production and proliferated uninhibited. Coactivation of Th1 and Th2 cells specific for the same Ag indicated that Th2 cells did not inhibit NO production, but were sensitive to NO induced by stimulated Th1 cells. Antigenic activation of Th2 cells in the presence of rIFN-gamma resulted in NO-mediated inhibition of proliferation. Th2 cells provided only a cell-associated cofactor, whereas Th1 cells secreted a soluble cofactor for IFN-gamma as well, i.e., TNF-alpha. Finally, a role for IFN-gamma and NO during immune responses was studied in spleen cells obtained from immunized IFN-gamma(-/-) mice. NO production and subsequent inhibition of Ag-specific proliferation ex vivo was observed only after the addition of rIFN-gamma. These studies suggest an IFN-gamma-dependent regulatory role for NO during Ag-specific Th cell activation involving macrophages, with obvious implications for Th subset-dependent immune responses in general. SN - 0008-8749 UR - https://www.unboundmedicine.com/medline/citation/11161444/Antigen_presentation_to_Th1_but_not_Th2_cells_by_macrophages_results_in_nitric_oxide_production_and_inhibition_of_T_cell_proliferation:_interferon_gamma_is_essential_but_insufficient_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-8749(00)91741-6 DB - PRIME DP - Unbound Medicine ER -