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Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo.
Dev Biol. 2001 Feb 15; 230(2):230-42.DB

Abstract

Endocardial cells are thought to contribute at least in part to the formation of the endocardial cushion mesenchyme. Here, we created Tie2-Cre transgenic mice, in which expression of Cre recombinase is driven by an endothelial-specific promoter/enhancer. To analyze the lineage of Cre expressing cells, we used CAG-CAT-Z transgenic mice, in which expression of lacZ is activated only after Cre-mediated recombination. We detected pan-endothelial expression of the Cre transgene in Tie2-Cre;CAG-CAT-Z double-transgenic mice. This expression pattern is almost identical to Tie2-lacZ transgenic mice. However, interestingly, we observed strong and uniform lacZ expression in mesenchymal cells of the atrioventricular canal of Tie2-Cre;CAG-CAT-Z double-transgenic mice. We also detected lacZ expression in the mesenchymal cells in part of the proximal cardiac outflow tract, but not in the mesenchymal cells of the distal outflow tract and branchial arch arteries. LacZ staining in Tie2-Cre;CAG-CAT-Z embryos is consistent with endocardial-mesenchymal transformation in the atrioventricular canal and outflow tract regions. Our observations are consistent with previously reported results from Cx43-lacZ, Wnt1-Cre;R26R, and Pax3-Cre;R26R transgenic mice, in which lacZ expression in the cardiac outflow tract identified contributions in part from the cardiac neural crest. Tie2-Cre transgenic mice are a new genetic tool for the analyses of endothelial cell-lineage and endothelial cell-specific gene targeting.

Authors+Show Affiliations

Department of Molecular Genetics, Howard Hughes Medical Institute, Dallas, Texas, 75390-9050, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11161575

Citation

Kisanuki, Y Y., et al. "Tie2-Cre Transgenic Mice: a New Model for Endothelial Cell-lineage Analysis in Vivo." Developmental Biology, vol. 230, no. 2, 2001, pp. 230-42.
Kisanuki YY, Hammer RE, Miyazaki J, et al. Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo. Dev Biol. 2001;230(2):230-42.
Kisanuki, Y. Y., Hammer, R. E., Miyazaki, J., Williams, S. C., Richardson, J. A., & Yanagisawa, M. (2001). Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo. Developmental Biology, 230(2), 230-42.
Kisanuki YY, et al. Tie2-Cre Transgenic Mice: a New Model for Endothelial Cell-lineage Analysis in Vivo. Dev Biol. 2001 Feb 15;230(2):230-42. PubMed PMID: 11161575.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tie2-Cre transgenic mice: a new model for endothelial cell-lineage analysis in vivo. AU - Kisanuki,Y Y, AU - Hammer,R E, AU - Miyazaki,J, AU - Williams,S C, AU - Richardson,J A, AU - Yanagisawa,M, PY - 2001/2/13/pubmed PY - 2001/4/6/medline PY - 2001/2/13/entrez SP - 230 EP - 42 JF - Developmental biology JO - Dev Biol VL - 230 IS - 2 N2 - Endocardial cells are thought to contribute at least in part to the formation of the endocardial cushion mesenchyme. Here, we created Tie2-Cre transgenic mice, in which expression of Cre recombinase is driven by an endothelial-specific promoter/enhancer. To analyze the lineage of Cre expressing cells, we used CAG-CAT-Z transgenic mice, in which expression of lacZ is activated only after Cre-mediated recombination. We detected pan-endothelial expression of the Cre transgene in Tie2-Cre;CAG-CAT-Z double-transgenic mice. This expression pattern is almost identical to Tie2-lacZ transgenic mice. However, interestingly, we observed strong and uniform lacZ expression in mesenchymal cells of the atrioventricular canal of Tie2-Cre;CAG-CAT-Z double-transgenic mice. We also detected lacZ expression in the mesenchymal cells in part of the proximal cardiac outflow tract, but not in the mesenchymal cells of the distal outflow tract and branchial arch arteries. LacZ staining in Tie2-Cre;CAG-CAT-Z embryos is consistent with endocardial-mesenchymal transformation in the atrioventricular canal and outflow tract regions. Our observations are consistent with previously reported results from Cx43-lacZ, Wnt1-Cre;R26R, and Pax3-Cre;R26R transgenic mice, in which lacZ expression in the cardiac outflow tract identified contributions in part from the cardiac neural crest. Tie2-Cre transgenic mice are a new genetic tool for the analyses of endothelial cell-lineage and endothelial cell-specific gene targeting. SN - 0012-1606 UR - https://www.unboundmedicine.com/medline/citation/11161575/Tie2_Cre_transgenic_mice:_a_new_model_for_endothelial_cell_lineage_analysis_in_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012160600901064 DB - PRIME DP - Unbound Medicine ER -