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Glutamine ameliorates mechanical obstruction-induced intestinal injury.
J Surg Res. 2001 Feb; 95(2):133-40.JS

Abstract

BACKGROUND

Glutamine has been shown to be an important dietary component for the maintenance of gut integrity. Although considered a nonessential amino acid in normal circumstances, glutamine may become conditionally essential for the bowel during episodes of severe illness and malnutrition. In this study, we employed an animal model simulating mechanical intestinal obstruction to explore the beneficial effects of glutamine on the intestine in response to obstruction-induced injury.

MATERIALS AND METHODS

Rats were on three feeding regimens-standard diet and water (control group), diet and water containing 2% glutamine (glutamine group), or diet and water containing 2% arginine (arginine group)-for 3 days prior to surgical preparation of intestinal obstruction. The bowel distension, fluid accumulation, and histological alterations in the intestinal mucosa were measured 40 h after ileal ligation.

RESULTS

After 3 days of drinking water intervention, the plasma glutamine levels in the glutamine group (677 +/- 12 microM) were higher than those in the control (451 +/- 27 microM) and arginine (379 +/- 25 microM) groups. The distension ratio measured 40 h after ileal ligation was significantly lower in the glutamine group (30.9 +/- 4.2%) than in the control and arginine groups (45.9 +/- 1.7 and 46.1 +/- 3.4%, respectively). Also, glutamine markedly decreased the fluid accumulation in the obstructed bowel segment (control group, 178.41 +/- 18.60 mg/cm; glutamine group, 104.97 +/- 13.17 mg/cm; arginine group, 141.4 +/- 12.85 mg/cm). Furthermore, the obstruction-induced mucosal injury was substantially improved in glutamine-fed rats.

CONCLUSIONS

Our findings indicate that glutamine can significantly reduce the degree of those physiological derangements induced by mechanical intestinal obstruction.

Authors+Show Affiliations

Department of Surgery, Tzu-Chi Buddhist General Hospital, Hua-Lien, Republic of China. tmchang@hcc.tcu.edu.twNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11162036

Citation

Chang, T, et al. "Glutamine Ameliorates Mechanical Obstruction-induced Intestinal Injury." The Journal of Surgical Research, vol. 95, no. 2, 2001, pp. 133-40.
Chang T, Lu R, Tsai L. Glutamine ameliorates mechanical obstruction-induced intestinal injury. J Surg Res. 2001;95(2):133-40.
Chang, T., Lu, R., & Tsai, L. (2001). Glutamine ameliorates mechanical obstruction-induced intestinal injury. The Journal of Surgical Research, 95(2), 133-40.
Chang T, Lu R, Tsai L. Glutamine Ameliorates Mechanical Obstruction-induced Intestinal Injury. J Surg Res. 2001;95(2):133-40. PubMed PMID: 11162036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutamine ameliorates mechanical obstruction-induced intestinal injury. AU - Chang,T, AU - Lu,R, AU - Tsai,L, PY - 2001/2/13/pubmed PY - 2001/3/27/medline PY - 2001/2/13/entrez SP - 133 EP - 40 JF - The Journal of surgical research JO - J Surg Res VL - 95 IS - 2 N2 - BACKGROUND: Glutamine has been shown to be an important dietary component for the maintenance of gut integrity. Although considered a nonessential amino acid in normal circumstances, glutamine may become conditionally essential for the bowel during episodes of severe illness and malnutrition. In this study, we employed an animal model simulating mechanical intestinal obstruction to explore the beneficial effects of glutamine on the intestine in response to obstruction-induced injury. MATERIALS AND METHODS: Rats were on three feeding regimens-standard diet and water (control group), diet and water containing 2% glutamine (glutamine group), or diet and water containing 2% arginine (arginine group)-for 3 days prior to surgical preparation of intestinal obstruction. The bowel distension, fluid accumulation, and histological alterations in the intestinal mucosa were measured 40 h after ileal ligation. RESULTS: After 3 days of drinking water intervention, the plasma glutamine levels in the glutamine group (677 +/- 12 microM) were higher than those in the control (451 +/- 27 microM) and arginine (379 +/- 25 microM) groups. The distension ratio measured 40 h after ileal ligation was significantly lower in the glutamine group (30.9 +/- 4.2%) than in the control and arginine groups (45.9 +/- 1.7 and 46.1 +/- 3.4%, respectively). Also, glutamine markedly decreased the fluid accumulation in the obstructed bowel segment (control group, 178.41 +/- 18.60 mg/cm; glutamine group, 104.97 +/- 13.17 mg/cm; arginine group, 141.4 +/- 12.85 mg/cm). Furthermore, the obstruction-induced mucosal injury was substantially improved in glutamine-fed rats. CONCLUSIONS: Our findings indicate that glutamine can significantly reduce the degree of those physiological derangements induced by mechanical intestinal obstruction. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/11162036/Glutamine_ameliorates_mechanical_obstruction_induced_intestinal_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022480400959833 DB - PRIME DP - Unbound Medicine ER -