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Oxidative stress and neuronal DNA fragmentation mediate age-dependent vulnerability to the mitochondrial toxin, 3-nitropropionic acid, in the mouse striatum.
Neurobiol Dis. 2001 Feb; 8(1):114-26.ND

Abstract

Oxidative stress is involved in the neuropathology of several neurodegenerative diseases and stroke, all of which are related to excitotoxicity. Age-dependent vulnerability is characteristic of these conditions. It is not clear whether apoptosis-related neuronal death is involved in age-dependent vulnerability to excitotoxicity. We evaluated whether apoptosis-related neuronal death after treatment with 3-nitropropionic acid (3-NP) is age-dependent in the mouse striatum. We have demonstrated that oxidative stress occurs early after 3-NP treatment and even more so in aged mice. DNA fragmentation with terminal deoxynucleotidyl transferase-mediated uridine 5'-triphosphate-biotin nick end labeling staining and gel electrophoresis occurred in an age-dependent fashion. Expression of the DNA repair enzyme, apurinic/apyrimidinic endonuclease, was more attenuated in old mice. Therefore, these results suggest that oxidative stress induces age-dependent neuronal apoptosis in the mouse striatum after 3-NP treatment, which in turn produces an age-dependent vulnerability to 3-NP.

Authors+Show Affiliations

Department of Neurosurgery, Stanford University School of Medicine, Palo Alto, California, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11162245

Citation

Kim, G W., and P H. Chan. "Oxidative Stress and Neuronal DNA Fragmentation Mediate Age-dependent Vulnerability to the Mitochondrial Toxin, 3-nitropropionic Acid, in the Mouse Striatum." Neurobiology of Disease, vol. 8, no. 1, 2001, pp. 114-26.
Kim GW, Chan PH. Oxidative stress and neuronal DNA fragmentation mediate age-dependent vulnerability to the mitochondrial toxin, 3-nitropropionic acid, in the mouse striatum. Neurobiol Dis. 2001;8(1):114-26.
Kim, G. W., & Chan, P. H. (2001). Oxidative stress and neuronal DNA fragmentation mediate age-dependent vulnerability to the mitochondrial toxin, 3-nitropropionic acid, in the mouse striatum. Neurobiology of Disease, 8(1), 114-26.
Kim GW, Chan PH. Oxidative Stress and Neuronal DNA Fragmentation Mediate Age-dependent Vulnerability to the Mitochondrial Toxin, 3-nitropropionic Acid, in the Mouse Striatum. Neurobiol Dis. 2001;8(1):114-26. PubMed PMID: 11162245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress and neuronal DNA fragmentation mediate age-dependent vulnerability to the mitochondrial toxin, 3-nitropropionic acid, in the mouse striatum. AU - Kim,G W, AU - Chan,P H, PY - 2001/2/13/pubmed PY - 2001/5/26/medline PY - 2001/2/13/entrez SP - 114 EP - 26 JF - Neurobiology of disease JO - Neurobiol Dis VL - 8 IS - 1 N2 - Oxidative stress is involved in the neuropathology of several neurodegenerative diseases and stroke, all of which are related to excitotoxicity. Age-dependent vulnerability is characteristic of these conditions. It is not clear whether apoptosis-related neuronal death is involved in age-dependent vulnerability to excitotoxicity. We evaluated whether apoptosis-related neuronal death after treatment with 3-nitropropionic acid (3-NP) is age-dependent in the mouse striatum. We have demonstrated that oxidative stress occurs early after 3-NP treatment and even more so in aged mice. DNA fragmentation with terminal deoxynucleotidyl transferase-mediated uridine 5'-triphosphate-biotin nick end labeling staining and gel electrophoresis occurred in an age-dependent fashion. Expression of the DNA repair enzyme, apurinic/apyrimidinic endonuclease, was more attenuated in old mice. Therefore, these results suggest that oxidative stress induces age-dependent neuronal apoptosis in the mouse striatum after 3-NP treatment, which in turn produces an age-dependent vulnerability to 3-NP. SN - 0969-9961 UR - https://www.unboundmedicine.com/medline/citation/11162245/Oxidative_stress_and_neuronal_DNA_fragmentation_mediate_age_dependent_vulnerability_to_the_mitochondrial_toxin_3_nitropropionic_acid_in_the_mouse_striatum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0969-9961(00)90327-9 DB - PRIME DP - Unbound Medicine ER -