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Oral administration of a nitric oxide synthase inhibitor enhances de novo mammalian angiogenesis mediated by TNF-alpha, saline and mast-cell secretion.
APMIS. 2000 Jul-Aug; 108(7-8):496-502.A

Abstract

In healthy adult rats, the test tissue that is used in the mesenteric-window angiogenesis assay is natively vascularized, lacks physiological angiogenesis, and is unperturbed by surgical intervention. Using the rat MWAA oral treatment with the nitric oxide (NO) synthase inhibitor Nw-nitro-L-arginine methyl ester (L-NAME) enhanced the angiogenic response (compared with controls receiving the inactive enantiomer D-NAME) following i.p. injections of (i) TNF-alpha at an approximate physiological dose, (ii) Compound 48/80, which is a highly selective secretagogue causing mast-cell secretion in situ and a very strong angiogenic response, and (iii) saline of a grade not made for infusion, causing a weak angiogenic response. Angiogenesis was assessed quantitatively using microscopic morphometry and image analysis in terms of objective variables recording the microvascular spatial extension, microvascular density, number and length of microvessel segments (extending between two successive branching points) and the degree of microvessel tortuosity. The data strongly suggest that endogenous NO inhibits all three mammalian angiogenesis reactions, although to a markedly different extent. Notably, the present data are virtually the opposite of those that have been reported from other mammalian angiogenesis models, the test tissues of which display deranged homeostasis, such as surgical intervention and/or ischemia.

Authors+Show Affiliations

Norrby K
Department of Pathology, Institute of Laboratory Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden. klas.norrby@path.gu.se

MeSH

Administration, OralAnimalsEnzyme InhibitorsMaleMammalsMast CellsNG-Nitroarginine Methyl EsterNeovascularization, PathologicNitric Oxide SynthaseRatsRats, Sprague-DawleySodium ChlorideTumor Necrosis Factor-alphaWeight Gainp-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11167545

Citation

Norrby, K. "Oral Administration of a Nitric Oxide Synthase Inhibitor Enhances De Novo Mammalian Angiogenesis Mediated By TNF-alpha, Saline and Mast-cell Secretion." APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, vol. 108, no. 7-8, 2000, pp. 496-502.
Norrby K. Oral administration of a nitric oxide synthase inhibitor enhances de novo mammalian angiogenesis mediated by TNF-alpha, saline and mast-cell secretion. APMIS. 2000;108(7-8):496-502.
Norrby, K. (2000). Oral administration of a nitric oxide synthase inhibitor enhances de novo mammalian angiogenesis mediated by TNF-alpha, saline and mast-cell secretion. APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 108(7-8), 496-502.
Norrby K. Oral Administration of a Nitric Oxide Synthase Inhibitor Enhances De Novo Mammalian Angiogenesis Mediated By TNF-alpha, Saline and Mast-cell Secretion. APMIS. 2000 Jul-Aug;108(7-8):496-502. PubMed PMID: 11167545.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oral administration of a nitric oxide synthase inhibitor enhances de novo mammalian angiogenesis mediated by TNF-alpha, saline and mast-cell secretion. A1 - Norrby,K, PY - 2001/2/13/pubmed PY - 2001/3/7/medline PY - 2001/2/13/entrez SP - 496 EP - 502 JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JO - APMIS VL - 108 IS - 7-8 N2 - In healthy adult rats, the test tissue that is used in the mesenteric-window angiogenesis assay is natively vascularized, lacks physiological angiogenesis, and is unperturbed by surgical intervention. Using the rat MWAA oral treatment with the nitric oxide (NO) synthase inhibitor Nw-nitro-L-arginine methyl ester (L-NAME) enhanced the angiogenic response (compared with controls receiving the inactive enantiomer D-NAME) following i.p. injections of (i) TNF-alpha at an approximate physiological dose, (ii) Compound 48/80, which is a highly selective secretagogue causing mast-cell secretion in situ and a very strong angiogenic response, and (iii) saline of a grade not made for infusion, causing a weak angiogenic response. Angiogenesis was assessed quantitatively using microscopic morphometry and image analysis in terms of objective variables recording the microvascular spatial extension, microvascular density, number and length of microvessel segments (extending between two successive branching points) and the degree of microvessel tortuosity. The data strongly suggest that endogenous NO inhibits all three mammalian angiogenesis reactions, although to a markedly different extent. Notably, the present data are virtually the opposite of those that have been reported from other mammalian angiogenesis models, the test tissues of which display deranged homeostasis, such as surgical intervention and/or ischemia. SN - 0903-4641 UR - https://www.unboundmedicine.com/medline/citation/11167545/Oral_administration_of_a_nitric_oxide_synthase_inhibitor_enhances_de_novo_mammalian_angiogenesis_mediated_by_TNF_alpha_saline_and_mast_cell_secretion_ DB - PRIME DP - Unbound Medicine ER -