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Pityrosporum and Candida specific and non-specific humoral, cellular and cytokine responses in atopic dermatitis patients.
Clin Exp Allergy. 2001 Jan; 31(1):125-34.CE

Abstract

BACKGROUND

Recent cytokine (RT-PCR, ELISA) analyses of inflammation in atopic dermatitis (AD) have suggested a role for IL-4, IL-5 and IFNgamma. Pityrosporum ovale and Candida albicans are important allergens in some patients with AD of the seborrhoic head, neck and shoulder region. In AD patients, the saprophytic yeasts induce IgE responses while they usually induce TH1 type responses. The cytokine responses induced by yeasts in AD are sparsely investigated.

OBJECTIVE

To characterize the P. ovale- and C. albicans-specific and non-specific humoral, lymphoproliferative and cytokine (IL-2, 4, 5 and IFNgamma) responses in AD.

METHODS

Fifteen AD patients and seven healthy controls (HC) were included. Ficoll-isolated PBMC were stimulated by PHA and laboratory-generated extracts of P. ovale and C. albicans. Lymphocyte proliferation was measured by 3H-thymidine incorporation and cytokine production by sandwich-ELISAs. The antigen-specific IgG and IgE antibodies were analysed by ELISA and nitrocellulose RAST.

RESULTS

Pityrosporum ovale- and C. albicans-specific IgE (both P < 0.001) and P. ovale-induced PBMC proliferation (P < 0.02) were elevated in AD. In general, the IL-4/IFNgamma ratio induced by P. ovale was higher than that induced by C. albicans (P < 0.01). The PHA-induced IL-2 (P < 0.05) and IL-4 responses (P < 0.005), and the C. albicans-induced IL-5 response (P < 0.02) and IFNgamma response (P < 0.01), were elevated in AD. A network of correlations was seen between serum total and the yeast-specific IgE, P. ovale-specific lymphoproliferation, PHA-induced IL-2, IL-4 and IL-5, and C. albicans-induced IL-5.

CONCLUSION

The cytokine profiles found in this study support the role of TH0 or TH1 cells by the side of TH2 cells in the pathogenesis of atopic dermatitis. Pityrosporum ovale appears to be associated more with IL-4 responses and C. albicans with IFNgamma responses.

Authors+Show Affiliations

Department of Pulmonary Diseases and Clinical Allergology, University of Turku, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11167960

Citation

Savolainen, J, et al. "Pityrosporum and Candida Specific and Non-specific Humoral, Cellular and Cytokine Responses in Atopic Dermatitis Patients." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 31, no. 1, 2001, pp. 125-34.
Savolainen J, Lintu P, Kosonen J, et al. Pityrosporum and Candida specific and non-specific humoral, cellular and cytokine responses in atopic dermatitis patients. Clin Exp Allergy. 2001;31(1):125-34.
Savolainen, J., Lintu, P., Kosonen, J., Kortekangas-Savolainen, O., Viander, M., Pène, J., Kalimo, K., Terho, E. O., & Bousquet, J. (2001). Pityrosporum and Candida specific and non-specific humoral, cellular and cytokine responses in atopic dermatitis patients. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 31(1), 125-34.
Savolainen J, et al. Pityrosporum and Candida Specific and Non-specific Humoral, Cellular and Cytokine Responses in Atopic Dermatitis Patients. Clin Exp Allergy. 2001;31(1):125-34. PubMed PMID: 11167960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pityrosporum and Candida specific and non-specific humoral, cellular and cytokine responses in atopic dermatitis patients. AU - Savolainen,J, AU - Lintu,P, AU - Kosonen,J, AU - Kortekangas-Savolainen,O, AU - Viander,M, AU - Pène,J, AU - Kalimo,K, AU - Terho,E O, AU - Bousquet,J, PY - 2001/2/13/pubmed PY - 2001/6/8/medline PY - 2001/2/13/entrez SP - 125 EP - 34 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 31 IS - 1 N2 - BACKGROUND: Recent cytokine (RT-PCR, ELISA) analyses of inflammation in atopic dermatitis (AD) have suggested a role for IL-4, IL-5 and IFNgamma. Pityrosporum ovale and Candida albicans are important allergens in some patients with AD of the seborrhoic head, neck and shoulder region. In AD patients, the saprophytic yeasts induce IgE responses while they usually induce TH1 type responses. The cytokine responses induced by yeasts in AD are sparsely investigated. OBJECTIVE: To characterize the P. ovale- and C. albicans-specific and non-specific humoral, lymphoproliferative and cytokine (IL-2, 4, 5 and IFNgamma) responses in AD. METHODS: Fifteen AD patients and seven healthy controls (HC) were included. Ficoll-isolated PBMC were stimulated by PHA and laboratory-generated extracts of P. ovale and C. albicans. Lymphocyte proliferation was measured by 3H-thymidine incorporation and cytokine production by sandwich-ELISAs. The antigen-specific IgG and IgE antibodies were analysed by ELISA and nitrocellulose RAST. RESULTS: Pityrosporum ovale- and C. albicans-specific IgE (both P < 0.001) and P. ovale-induced PBMC proliferation (P < 0.02) were elevated in AD. In general, the IL-4/IFNgamma ratio induced by P. ovale was higher than that induced by C. albicans (P < 0.01). The PHA-induced IL-2 (P < 0.05) and IL-4 responses (P < 0.005), and the C. albicans-induced IL-5 response (P < 0.02) and IFNgamma response (P < 0.01), were elevated in AD. A network of correlations was seen between serum total and the yeast-specific IgE, P. ovale-specific lymphoproliferation, PHA-induced IL-2, IL-4 and IL-5, and C. albicans-induced IL-5. CONCLUSION: The cytokine profiles found in this study support the role of TH0 or TH1 cells by the side of TH2 cells in the pathogenesis of atopic dermatitis. Pityrosporum ovale appears to be associated more with IL-4 responses and C. albicans with IFNgamma responses. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/11167960/Pityrosporum_and_Candida_specific_and_non_specific_humoral_cellular_and_cytokine_responses_in_atopic_dermatitis_patients_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=0954-7894&amp;date=2001&amp;volume=31&amp;issue=1&amp;spage=125 DB - PRIME DP - Unbound Medicine ER -