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Expression of telomerase genes correlates with telomerase activity in human colorectal carcinogenesis.
J Pathol. 2001 Jan; 193(1):21-6.JP

Abstract

The human telomerase enzyme is composed of two essential components, hTR, which acts as a template for reverse transcription, and hTERT, which is the putative catalytic subunit for the enzyme. Recent studies have demonstrated a good correlation between hTERT expression and telomerase activation, whereas RT-PCR results seemed to reveal that hTR is ubiquitously expressed in all cells. These observations left unclear the role of hTR, and to a lesser extent hTERT, in the regulation of telomerase activation. In the present study, the correlation of telomerase activity and the expression of these genes was examined in a total of 70 colorectal tissues (25 adenocarcinomas, 30 adenomas, and 15 samples of normal colorectal mucosa). Total RNA for RT-PCR analysis and cell extracts for TRAP assay were obtained from consecutive sections and histological control was simultaneously performed. To avoid false-positive results, due to the fact that hTR cDNA and genomic hTR DNA are identical (the gene has no introns), extensive DNase digestion was performed before cDNA synthesis. RT-PCR analysis revealed that hTERT mRNA was expressed in all cancers and in 13 of 14 telomerase-positive adenomas, but never in telomerase-negative colorectal tissues. hTR transcripts were observed in all telomerase-positive samples but also in three telomerase-negative samples, two adenomas, and one normal colonic mucosa. It is concluded that hTERT and hTR expression is strongly correlated with telomerase activity. hTR transcripts, however, also occur in some telomerase-negative tissues and these results are in keeping with the concept that hTERT expression is a major regulator of telomerase activity.

Authors+Show Affiliations

Institute of Pathology, Centre Hospitalier Universitaire Vaudois, CH-1011 Lausanne, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11169511

Citation

Yan, P, et al. "Expression of Telomerase Genes Correlates With Telomerase Activity in Human Colorectal Carcinogenesis." The Journal of Pathology, vol. 193, no. 1, 2001, pp. 21-6.
Yan P, Saraga EP, Bouzourene H, et al. Expression of telomerase genes correlates with telomerase activity in human colorectal carcinogenesis. J Pathol. 2001;193(1):21-6.
Yan, P., Saraga, E. P., Bouzourene, H., Bosman, F. T., & Benhattar, J. (2001). Expression of telomerase genes correlates with telomerase activity in human colorectal carcinogenesis. The Journal of Pathology, 193(1), 21-6.
Yan P, et al. Expression of Telomerase Genes Correlates With Telomerase Activity in Human Colorectal Carcinogenesis. J Pathol. 2001;193(1):21-6. PubMed PMID: 11169511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of telomerase genes correlates with telomerase activity in human colorectal carcinogenesis. AU - Yan,P, AU - Saraga,E P, AU - Bouzourene,H, AU - Bosman,F T, AU - Benhattar,J, PY - 2001/2/13/pubmed PY - 2001/3/17/medline PY - 2001/2/13/entrez SP - 21 EP - 6 JF - The Journal of pathology JO - J Pathol VL - 193 IS - 1 N2 - The human telomerase enzyme is composed of two essential components, hTR, which acts as a template for reverse transcription, and hTERT, which is the putative catalytic subunit for the enzyme. Recent studies have demonstrated a good correlation between hTERT expression and telomerase activation, whereas RT-PCR results seemed to reveal that hTR is ubiquitously expressed in all cells. These observations left unclear the role of hTR, and to a lesser extent hTERT, in the regulation of telomerase activation. In the present study, the correlation of telomerase activity and the expression of these genes was examined in a total of 70 colorectal tissues (25 adenocarcinomas, 30 adenomas, and 15 samples of normal colorectal mucosa). Total RNA for RT-PCR analysis and cell extracts for TRAP assay were obtained from consecutive sections and histological control was simultaneously performed. To avoid false-positive results, due to the fact that hTR cDNA and genomic hTR DNA are identical (the gene has no introns), extensive DNase digestion was performed before cDNA synthesis. RT-PCR analysis revealed that hTERT mRNA was expressed in all cancers and in 13 of 14 telomerase-positive adenomas, but never in telomerase-negative colorectal tissues. hTR transcripts were observed in all telomerase-positive samples but also in three telomerase-negative samples, two adenomas, and one normal colonic mucosa. It is concluded that hTERT and hTR expression is strongly correlated with telomerase activity. hTR transcripts, however, also occur in some telomerase-negative tissues and these results are in keeping with the concept that hTERT expression is a major regulator of telomerase activity. SN - 0022-3417 UR - https://www.unboundmedicine.com/medline/citation/11169511/Expression_of_telomerase_genes_correlates_with_telomerase_activity_in_human_colorectal_carcinogenesis_ L2 - https://doi.org/10.1002/1096-9896(2000)9999:9999<::AID-PATH728>3.0.CO;2-G DB - PRIME DP - Unbound Medicine ER -