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Properties and inhibition of the first two enzymes of the non-mevalonate pathway of isoprenoid biosynthesis.
Biochem Soc Trans. 2000 Dec; 28(6):792-3.BS

Abstract

Enzymes of the 1-deoxy-D-xylulose 5-phosphate/2-C-methylerythritol 4-phosphate (DOXP/MEP) pathway are targets for new herbicides and antibacterial drugs. Until now, no inhibitors for the DOXP synthase have been known of. We show that one of the breakdown products of the herbicide clomazone affects the DOXP synthase. One inhibitor of the non-mevalonate pathway, fosmidomycin, blocks the DOXP reductoisomerase (DXR) of plants and bacteria. The I(50) values of plants are, however, higher than those found for the DXR of Escherichia coli. The DXR of plants, isolated from barley seedlings, shows a pH optimum of 8.1, which is typical for enzymes active in the chloroplast stroma.

Authors+Show Affiliations

Mueller C
Botanical Institute II, University of Karlsruhe, Kaiserstr. 12, D-76128 Karlsruhe, Germany.
Schwender J
No affiliation info available
Zeidler J
No affiliation info available
Lichtenthaler HK
No affiliation info available

MeSH

Aldose-Ketose IsomerasesAnimalsArabidopsisChlamydomonasFosfomycinHerbicidesHordeumIsoxazolesKineticsMultienzyme ComplexesOxazolidinonesOxidoreductasesTransferases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11171210

Citation

Mueller, C, et al. "Properties and Inhibition of the First Two Enzymes of the Non-mevalonate Pathway of Isoprenoid Biosynthesis." Biochemical Society Transactions, vol. 28, no. 6, 2000, pp. 792-3.
Mueller C, Schwender J, Zeidler J, et al. Properties and inhibition of the first two enzymes of the non-mevalonate pathway of isoprenoid biosynthesis. Biochem Soc Trans. 2000;28(6):792-3.
Mueller, C., Schwender, J., Zeidler, J., & Lichtenthaler, H. K. (2000). Properties and inhibition of the first two enzymes of the non-mevalonate pathway of isoprenoid biosynthesis. Biochemical Society Transactions, 28(6), 792-3.
Mueller C, et al. Properties and Inhibition of the First Two Enzymes of the Non-mevalonate Pathway of Isoprenoid Biosynthesis. Biochem Soc Trans. 2000;28(6):792-3. PubMed PMID: 11171210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Properties and inhibition of the first two enzymes of the non-mevalonate pathway of isoprenoid biosynthesis. AU - Mueller,C, AU - Schwender,J, AU - Zeidler,J, AU - Lichtenthaler,H K, PY - 2001/2/15/pubmed PY - 2001/6/2/medline PY - 2001/2/15/entrez SP - 792 EP - 3 JF - Biochemical Society transactions JO - Biochem Soc Trans VL - 28 IS - 6 N2 - Enzymes of the 1-deoxy-D-xylulose 5-phosphate/2-C-methylerythritol 4-phosphate (DOXP/MEP) pathway are targets for new herbicides and antibacterial drugs. Until now, no inhibitors for the DOXP synthase have been known of. We show that one of the breakdown products of the herbicide clomazone affects the DOXP synthase. One inhibitor of the non-mevalonate pathway, fosmidomycin, blocks the DOXP reductoisomerase (DXR) of plants and bacteria. The I(50) values of plants are, however, higher than those found for the DXR of Escherichia coli. The DXR of plants, isolated from barley seedlings, shows a pH optimum of 8.1, which is typical for enzymes active in the chloroplast stroma. SN - 0300-5127 UR - https://www.unboundmedicine.com/medline/citation/11171210/Properties_and_inhibition_of_the_first_two_enzymes_of_the_non_mevalonate_pathway_of_isoprenoid_biosynthesis_ DB - PRIME DP - Unbound Medicine ER -