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Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana.
Biochem Soc Trans. 2000 Dec; 28(6):947-50.BS

Abstract

Seed-specific expression in Arabidopsis thaliana of oleate hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of hydroxy fatty acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n-3 desaturase (FAD3) and the FAE1 condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain hydroxy fatty acids, respectively. In Arabidopsis plants with an active ER Delta12-oleate desaturase the presence of hydroxy fatty acids corresponded to an increase in the levels of 18:1 and a decrease in 18:2 levels. Expression in yeast indicates that the castor hydroxylase also has a low level of desaturase activity.

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Authors+Show Affiliations

Smith M
Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, BC, V6T 1Z4, Canada. msmith@mail.botany.ubc.ca
Moon H
No affiliation info available
Kunst L
No affiliation info available

MeSH

ArabidopsisCastor BeanFatty Acid DesaturasesFatty AcidsHydroxy AcidsMixed Function OxygenasesPlant ProteinsPlants, Genetically ModifiedPlants, ToxicSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11171267

Citation

Smith, M, et al. "Production of Hydroxy Fatty Acids in the Seeds of Arabidopsis Thaliana." Biochemical Society Transactions, vol. 28, no. 6, 2000, pp. 947-50.
Smith M, Moon H, Kunst L. Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana. Biochem Soc Trans. 2000;28(6):947-50.
Smith, M., Moon, H., & Kunst, L. (2000). Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana. Biochemical Society Transactions, 28(6), 947-50.
Smith M, Moon H, Kunst L. Production of Hydroxy Fatty Acids in the Seeds of Arabidopsis Thaliana. Biochem Soc Trans. 2000;28(6):947-50. PubMed PMID: 11171267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana. AU - Smith,M, AU - Moon,H, AU - Kunst,L, PY - 2001/2/15/pubmed PY - 2001/6/2/medline PY - 2001/2/15/entrez SP - 947 EP - 50 JF - Biochemical Society transactions JO - Biochem Soc Trans VL - 28 IS - 6 N2 - Seed-specific expression in Arabidopsis thaliana of oleate hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of hydroxy fatty acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n-3 desaturase (FAD3) and the FAE1 condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain hydroxy fatty acids, respectively. In Arabidopsis plants with an active ER Delta12-oleate desaturase the presence of hydroxy fatty acids corresponded to an increase in the levels of 18:1 and a decrease in 18:2 levels. Expression in yeast indicates that the castor hydroxylase also has a low level of desaturase activity. SN - 0300-5127 UR - https://www.unboundmedicine.com/medline/citation/11171267/Production_of_hydroxy_fatty_acids_in_the_seeds_of_Arabidopsis_thaliana_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:11171267 DB - PRIME DP - Unbound Medicine ER -