Tags

Type your tag names separated by a space and hit enter

Sonic hedgehog is a survival factor for hypaxial muscles during mouse development.
Development. 2001 Mar; 128(5):743-52.D

Abstract

Sonic hedgehog (Shh) has been proposed to function as an inductive and trophic signal that controls development of epaxial musculature in vertebrate embryos. In contrast, development of hypaxial muscles was assumed to occur independently of Shh. We here show that formation of limb muscles was severely affected in two different mouse strains with inactivating mutations of the Shh gene. The limb muscle defect became apparent relatively late and initial stages of hypaxial muscle development were unaffected or only slightly delayed. Micromass cultures and cultures of tissue fragments derived from limbs under different conditions with or without the overlaying ectoderm indicated that Shh is required for the maintenance of the expression of myogenic regulatory factors (MRFs) and, consecutively, for the formation of differentiated limb muscle myotubes. We propose that Shh acts as a survival and proliferation factor for myogenic precursor cells during hypaxial muscle development. Detection of a reduced but significant level of Myf5 expression in the epaxial compartment of somites of Shh homozygous mutant embryos at E9.5 indicated that Shh might be dispensable for the initiation of myogenesis both in hypaxial and epaxial muscles. Our data suggest that Shh acts similarly in both somitic compartments as a survival and proliferation factor and not as a primary inducer of myogenesis.

Authors+Show Affiliations

Institute of Physiological Chemistry, University of Halle-Wittenberg, Hollystr. 1, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11171399

Citation

Krüger, M, et al. "Sonic Hedgehog Is a Survival Factor for Hypaxial Muscles During Mouse Development." Development (Cambridge, England), vol. 128, no. 5, 2001, pp. 743-52.
Krüger M, Mennerich D, Fees S, et al. Sonic hedgehog is a survival factor for hypaxial muscles during mouse development. Development. 2001;128(5):743-52.
Krüger, M., Mennerich, D., Fees, S., Schäfer, R., Mundlos, S., & Braun, T. (2001). Sonic hedgehog is a survival factor for hypaxial muscles during mouse development. Development (Cambridge, England), 128(5), 743-52.
Krüger M, et al. Sonic Hedgehog Is a Survival Factor for Hypaxial Muscles During Mouse Development. Development. 2001;128(5):743-52. PubMed PMID: 11171399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sonic hedgehog is a survival factor for hypaxial muscles during mouse development. AU - Krüger,M, AU - Mennerich,D, AU - Fees,S, AU - Schäfer,R, AU - Mundlos,S, AU - Braun,T, PY - 2001/2/15/pubmed PY - 2001/4/17/medline PY - 2001/2/15/entrez SP - 743 EP - 52 JF - Development (Cambridge, England) JO - Development VL - 128 IS - 5 N2 - Sonic hedgehog (Shh) has been proposed to function as an inductive and trophic signal that controls development of epaxial musculature in vertebrate embryos. In contrast, development of hypaxial muscles was assumed to occur independently of Shh. We here show that formation of limb muscles was severely affected in two different mouse strains with inactivating mutations of the Shh gene. The limb muscle defect became apparent relatively late and initial stages of hypaxial muscle development were unaffected or only slightly delayed. Micromass cultures and cultures of tissue fragments derived from limbs under different conditions with or without the overlaying ectoderm indicated that Shh is required for the maintenance of the expression of myogenic regulatory factors (MRFs) and, consecutively, for the formation of differentiated limb muscle myotubes. We propose that Shh acts as a survival and proliferation factor for myogenic precursor cells during hypaxial muscle development. Detection of a reduced but significant level of Myf5 expression in the epaxial compartment of somites of Shh homozygous mutant embryos at E9.5 indicated that Shh might be dispensable for the initiation of myogenesis both in hypaxial and epaxial muscles. Our data suggest that Shh acts similarly in both somitic compartments as a survival and proliferation factor and not as a primary inducer of myogenesis. SN - 0950-1991 UR - https://www.unboundmedicine.com/medline/citation/11171399/Sonic_hedgehog_is_a_survival_factor_for_hypaxial_muscles_during_mouse_development_ L2 - http://dev.biologists.org/cgi/pmidlookup?view=long&pmid=11171399 DB - PRIME DP - Unbound Medicine ER -